| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,004,409 | T→C | 57.2% | T396A (ACC→GCC) | pntA ← | pyridine nucleotide transhydrogenase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,004,409 | 0 | T | C | 57.2% | 4.8 / 17.2 | 28 | T396A (ACC→GCC) | pntA | pyridine nucleotide transhydrogenase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (7/5); new base C (10/6); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAA > minE/1004351‑1004477 | cAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTc > 1:2856016/1‑68 (MQ=255) tGATTGCCAACGCCATCAACGCGTATTTACGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACtt < 1:1184351/69‑1 (MQ=255) tGATTGCCAACGCCATCAACGCGTATTTACGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACtt < 1:1289870/69‑1 (MQ=255) tGATTGCCAACGCCATCAACGCGTATTTACGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACtt < 1:1656535/69‑1 (MQ=255) tGATTGCCAACGCCATCAACGCGTATTTACGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACtt < 1:3136136/69‑1 (MQ=255) tGATTGCCAACGCCATCAACGCGTATTTACGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACtt < 1:2966570/69‑1 (MQ=255) gATTGCCAACGCCATCAACGCGTATTTACGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACtt < 1:2786118/68‑1 (MQ=255) aaCGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGcc > 1:625316/1‑60 (MQ=255) aaCGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTtgt > 1:1677835/1‑69 (MQ=255) aaCGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTtgt > 1:1044220/1‑69 (MQ=255) aaCGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTtgt > 1:906233/1‑69 (MQ=255) aaCGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTtgt > 1:758719/1‑69 (MQ=255) aaCGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTtgt > 1:248474/1‑69 (MQ=255) tACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCg < 1:91998/69‑1 (MQ=255) tACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCg < 1:3259595/69‑1 (MQ=255) tACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCg < 1:2237296/69‑1 (MQ=255) tACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCg < 1:1880823/69‑1 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTGAGTTTTCACTTCCGGTGCCGCtt > 1:2334961/1‑53 (MQ=39) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:2654772/1‑54 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:2357483/1‑54 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:2924256/1‑54 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:3105130/1‑54 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:3250350/1‑54 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:1986469/1‑54 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:702193/1‑54 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:1957711/1‑54 (MQ=255) aCGCCACGGTGAGCAGGCACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCttt > 1:1774074/1‑54 (MQ=255) aCGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCg < 1:2035578/63‑1 (MQ=255) tACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGaa > 1:2587009/1‑69 (MQ=255) tACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGaa > 1:2519945/1‑69 (MQ=255) tACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGa > 1:568316/1‑68 (MQ=255) | CAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAA > minE/1004351‑1004477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |