| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 88,344 | A→T | 38.2% | E150D (GAA→GAT) | ftsQ → | membrane anchored protein involved in growth of wall at septum |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 88,344 | 0 | A | T | 38.2% | 49.3 / 56.3 | 68 | E150D (GAA→GAT) | ftsQ | membrane anchored protein involved in growth of wall at septum |
| Reads supporting (aligned to +/- strand): ref base A (16/26); new base T (18/8); total (34/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGG > minE/88300‑88411 | cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:2674681/68‑1 (MQ=255)cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:1926438/68‑1 (MQ=255)cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:1865816/68‑1 (MQ=255)cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:1593664/68‑1 (MQ=255)cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:1537226/68‑1 (MQ=255)cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:1523333/68‑1 (MQ=255)cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:687570/68‑1 (MQ=255)cATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:3046713/68‑1 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa > 1:1885906/1‑66 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa > 1:2078076/1‑66 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa > 1:1885567/1‑66 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa > 1:2285531/1‑66 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa > 1:3028822/1‑66 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa > 1:1846563/1‑66 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa > 1:1861058/1‑66 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCaa > 1:3358549/1‑66 (MQ=255) tGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGc > 1:164286/1‑69 (MQ=255) aCGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTcc < 1:2310553/58‑1 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATgg > 1:1236687/1‑68 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:230162/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:217177/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:2225801/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:1950438/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:549523/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:2313267/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:2408389/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:159688/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:1293200/1‑69 (MQ=255) gcgGAAGGAAACACCTTCAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGc > 1:3315326/1‑69 (MQ=255) ccTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAgc > 1:2278623/1‑68 (MQ=255) cAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCa > 1:2875644/1‑68 (MQ=255) cAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCa > 1:2996687/1‑68 (MQ=255) cAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCa > 1:3093026/1‑68 (MQ=255) cAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCa > 1:1117705/1‑68 (MQ=255) cAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCa > 1:2209703/1‑68 (MQ=255) cAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCa > 1:1456143/1‑68 (MQ=255) cAGCGTGCCGCCAGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCa > 1:1942256/1‑68 (MQ=255) gccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCATTGAAgt < 1:751/69‑1 (MQ=255) gccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgt < 1:3270355/69‑1 (MQ=255) gccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgt < 1:1012108/69‑1 (MQ=255) gccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgt < 1:2335554/69‑1 (MQ=255) gccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgt < 1:1299671/69‑1 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtg > 1:45938/1‑69 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtg > 1:2119347/1‑69 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtg < 1:1882673/69‑1 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtg > 1:448934/1‑69 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtg > 1:2697342/1‑69 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtg > 1:2742514/1‑69 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtg > 1:1406839/1‑69 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgt < 1:561656/68‑1 (MQ=255) ccgccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgt < 1:2163311/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:1378797/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:2198626/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:2993689/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:2901104/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:2800546/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:2531541/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:251642/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:55268/68‑1 (MQ=255) gccAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAgtgt < 1:490847/68‑1 (MQ=255) aGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa < 1:607064/69‑1 (MQ=255) aGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa < 1:891483/69‑1 (MQ=255) aGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa < 1:3284115/69‑1 (MQ=255) aGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa < 1:2282014/69‑1 (MQ=255) aGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa < 1:2035441/69‑1 (MQ=255) aGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa < 1:1906030/69‑1 (MQ=255) aGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa < 1:145240/69‑1 (MQ=255) aGATCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCa < 1:1132102/69‑1 (MQ=255) aaCGCACCAGCAAGCTGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAgg < 1:228187/69‑1 (MQ=255) aaCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAgg < 1:3091856/69‑1 (MQ=255) aaCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAgg < 1:5593/69‑1 (MQ=255) aaCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAgg < 1:1528287/69‑1 (MQ=255) aaCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAgg < 1:1481740/69‑1 (MQ=255) aaCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAgg < 1:886876/69‑1 (MQ=255) aaCGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCACCGCCAATGAAGTGTTGCAgg < 1:1945778/69‑1 (MQ=255) | CATATGGTAGACGCGGAAGGAAATACCTTCAGCGTGCCGCCAGAACGCACCAGCAAGCAGGTGCTTCCAATGCTGTATGGCCCGGAAGGCAGCGCCAATGAAGTGTTGCAGG > minE/88300‑88411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |