| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,005,190 | G→A | 32.1% | N135N (AAC→AAT) | pntA ← | pyridine nucleotide transhydrogenase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,005,190 | 0 | G | A | 32.1% | 76.9 / 43.1 | 87 | N135N (AAC→AAT) | pntA | pyridine nucleotide transhydrogenase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (18/41); new base A (22/6); total (40/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.05e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATACGC > minE/1005125‑1005238 | aTTTGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTc > 1:300208/1‑69 (MQ=255)aTTTGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTc > 1:2077038/1‑69 (MQ=255)aTTTGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTc > 1:2446057/1‑69 (MQ=255) ttGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGc < 1:2462719/69‑1 (MQ=255) ttGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGc < 1:3432698/69‑1 (MQ=255) tGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGcc > 1:906170/1‑69 (MQ=255) tGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGcc > 1:298412/1‑69 (MQ=255) tGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGcc > 1:2696105/1‑69 (MQ=255) tGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGcc > 1:2680242/1‑69 (MQ=255) tGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGcc > 1:2204175/1‑69 (MQ=255) tGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGc > 1:2848273/1‑68 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCa > 1:1624577/1‑62 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAg > 1:2660288/1‑67 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAg > 1:1056274/1‑67 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAg > 1:2404956/1‑67 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAg > 1:794419/1‑67 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAg > 1:1734478/1‑67 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCt < 1:2274202/69‑1 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCt < 1:191143/69‑1 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCt < 1:1796381/69‑1 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCt < 1:1393198/69‑1 (MQ=255) aaaGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCt < 1:725214/69‑1 (MQ=255) aagaagCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCtt < 1:309007/69‑1 (MQ=255) aagaagCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCt < 1:2794472/68‑1 (MQ=255) agaagCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTa > 1:2834042/1‑69 (MQ=255) agaagCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTa > 1:2056257/1‑69 (MQ=255) agaagCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTa > 1:1585948/1‑69 (MQ=255) agaagCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTa > 1:3164750/1‑69 (MQ=255) agaagCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTa > 1:212901/1‑69 (MQ=255) agaagCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTa > 1:1188059/1‑69 (MQ=255) aTTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCg < 1:1738027/69‑1 (MQ=255) aTTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCg < 1:2228729/69‑1 (MQ=255) aTTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCg < 1:531238/69‑1 (MQ=255) aTTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCg < 1:2472177/69‑1 (MQ=255) aTTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCg < 1:541037/69‑1 (MQ=255) aTTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCg < 1:353794/69‑1 (MQ=255) aTTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCg < 1:2938090/69‑1 (MQ=255) aTTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTACGTCCAGCg < 1:1940453/69‑1 (MQ=255) ttCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCg < 1:2578804/68‑1 (MQ=255) tCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGAt < 1:2205871/69‑1 (MQ=255) tCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGAt < 1:555458/69‑1 (MQ=255) tCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGAt < 1:3376590/69‑1 (MQ=255) tCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGAt < 1:1140180/69‑1 (MQ=255) tCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGAt < 1:2816637/69‑1 (MQ=255) tCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGAt < 1:2820674/69‑1 (MQ=255) cATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGAtt > 1:2412029/1‑69 (MQ=255) cATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGAt < 1:2684363/68‑1 (MQ=255) ccGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:138815/69‑1 (MQ=255) ccGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:452579/69‑1 (MQ=255) ccGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:3313042/69‑1 (MQ=255) ccGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:3257720/69‑1 (MQ=255) ccGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:702933/69‑1 (MQ=255) ccGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:1612221/69‑1 (MQ=255) ccGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:2431537/69‑1 (MQ=255) ccGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:208897/69‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:1817727/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:1714366/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:1639762/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:2829099/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:543056/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:2270897/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:613629/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:329080/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:1482921/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:334053/67‑1 (MQ=255) acaaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:2135637/67‑1 (MQ=255) caaTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:1653243/66‑1 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGAtt > 1:1744825/1‑53 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:577205/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:776197/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:622967/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:726596/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:1180428/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:1065958/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:1355164/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:444372/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:334847/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:1504576/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:3132635/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:303456/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:1981900/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:2094517/1‑69 (MQ=255) aaTGGCGCGATAACCGGCGATATTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcg > 1:2178057/1‑69 (MQ=255) aTGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcgc > 1:1404169/1‑69 (MQ=255) aTGGCGCGATAACCGGCGATGTTCACCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATAcgc > 1:2134559/1‑69 (MQ=255) gcgATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTgcgc < 1:733974/53‑1 (MQ=255) cGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTgaga < 1:325161/48‑1 (MQ=255) | ATTTGCCCGGTAAAGAAGCGCCCAAATTCATGTGCCGCTTCAACAATGGCGCGATAACCGGCGATGTTCGCCATCGAGCTTAGTGCGTCCAGCGATTGTGCGCGTGAGATACGC > minE/1005125‑1005238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |