| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,008,927 | T→C | 34.7% | S87S (AGT→AGC) | folM → | dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,008,927 | 0 | T | C | 34.7% | 63.1 / 53.1 | 92 | S87S (AGT→AGC) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base T (43/17); new base C (19/13); total (62/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01 | |||||||||||
GTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATC > minE/1008859‑1008990 | gtgATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGt > 1:3227910/1‑69 (MQ=255)gtgATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGt > 1:2048267/1‑69 (MQ=255)gtgATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGt > 1:1237848/1‑69 (MQ=255)gtgATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGt > 1:2417255/1‑69 (MQ=255)gtgATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGt > 1:1292450/1‑69 (MQ=255)gtgATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGt > 1:1319137/1‑69 (MQ=255) ggCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTg < 1:3116149/69‑1 (MQ=255) ggCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTg < 1:2746461/69‑1 (MQ=255) tGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATgg > 1:700896/1‑68 (MQ=255) gTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAAcc < 1:1527269/68‑1 (MQ=255) gTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAAcc < 1:2523131/68‑1 (MQ=255) gTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAAcc < 1:1571248/68‑1 (MQ=255) tACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTg < 1:3276392/52‑1 (MQ=255) tACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCgg > 1:3333870/1‑69 (MQ=255) tACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCg > 1:2007075/1‑68 (MQ=255) cACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACt > 1:932150/1‑69 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:245597/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:1829886/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:2093638/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:1422577/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:938481/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:641244/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:3336428/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:1425/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:559519/69‑3 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGacgacg > 1:3365545/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGacgacg > 1:3369426/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:246253/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:615102/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:219871/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:2233183/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:511645/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:454110/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:417861/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:2646691/69‑1 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:2827655/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:3316496/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:10038/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1318979/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1934117/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1351346/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1307946/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1068601/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1502683/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1753047/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:1161178/69‑1 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1610763/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAAAGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:31869/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAACCCGGGTGCGCCACTGGCCGACGt > 1:2717526/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:3406288/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:504548/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:157002/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:2097180/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:2099654/1‑69 (MQ=255) cTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTa < 1:1003554/69‑1 (MQ=255) aTTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:1548996/68‑1 (MQ=255) aTTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:2879330/68‑1 (MQ=255) aTTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:3147421/68‑1 (MQ=255) aTTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:3256018/68‑1 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAAcc > 1:2495191/1‑38 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTg > 1:1159466/1‑68 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1234763/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1326434/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1327178/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:343235/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1301324/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1450130/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1060405/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:3158689/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:3154673/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1458919/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:2976054/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1922855/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:2838185/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:2269492/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1243061/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1809470/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCAACTGACCGACGTACTGGCTTGc > 1:976639/1‑69 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:2411775/68‑1 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:486359/68‑1 (MQ=255) tAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGc < 1:1369277/68‑1 (MQ=255) tAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGc < 1:1927501/68‑1 (MQ=255) tAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGc < 1:1630897/68‑1 (MQ=255) tAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGc < 1:3305723/68‑1 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCg > 1:2018385/1‑46 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:2946697/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:507561/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:2691728/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:2625120/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:1371336/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:3377129/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:895277/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:899144/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:922923/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:2107127/1‑64 (MQ=255) aCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGa > 1:1942517/1‑69 (MQ=255) gCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:463419/69‑1 (MQ=255) gCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:709539/69‑1 (MQ=255) | GTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATC > minE/1008859‑1008990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |