| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,008,951 | T→A | 26.6% | G95G (GGT→GGA) | folM → | dihydrofolate reductase isozyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,008,951 | 0 | T | A | 26.6% | 134.4 / 66.2 | 117 | G95G (GGT→GGA) | folM | dihydrofolate reductase isozyme |
| Reads supporting (aligned to +/- strand): ref base T (45/41); new base A (18/13); total (63/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-04 | |||||||||||
Only 100 of 117 total aligned reads displayed.
CACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAAC > minE/1008891‑1009017 | cACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACt > 1:932150/1‑69 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:3336428/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:1425/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:1829886/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:641244/69‑3 (MQ=255) ccATGGTCTGCGTGCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTgac < 1:938481/69‑3 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGacgacg > 1:3369426/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGacgacg > 1:3365545/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1502683/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:10038/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1753047/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1934117/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:219871/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:2233183/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:246253/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:2646691/69‑1 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:2827655/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:615102/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:511645/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:417861/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:454110/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1318979/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1068601/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:1351346/1‑69 (MQ=255) gTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:1161178/69‑1 (MQ=255) gTCTGCGTGCTATTTTGCATAAAGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg > 1:31869/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAACCCGGGTGCGCCACTGGCCGACGt > 1:2717526/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:157002/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:504548/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:3406288/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:2099654/1‑69 (MQ=255) tCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGt > 1:2097180/1‑69 (MQ=255) cTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTa < 1:1003554/69‑1 (MQ=255) aTTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:1548996/68‑1 (MQ=255) aTTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:2879330/68‑1 (MQ=255) aTTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:3147421/68‑1 (MQ=255) aTTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTg < 1:3256018/68‑1 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:2976054/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:3154673/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1234763/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:343235/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:2838185/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1243061/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1060405/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1458919/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1301324/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1450130/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1809470/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1922855/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:2269492/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1326434/1‑69 (MQ=255) aTTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGc > 1:1327178/1‑69 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:486359/68‑1 (MQ=255) ttGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCat < 1:2411775/68‑1 (MQ=255) tAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGc < 1:1927501/68‑1 (MQ=255) tAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGc < 1:1630897/68‑1 (MQ=255) tAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGc < 1:3305723/68‑1 (MQ=255) tAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGc < 1:1369277/68‑1 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCg > 1:2018385/1‑46 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:2107127/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:895277/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:3377129/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:1371336/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:507561/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:2625120/1‑64 (MQ=255) aaCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCatga > 1:922923/1‑64 (MQ=255) aCGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGa > 1:1942517/1‑69 (MQ=255) gCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:709539/69‑1 (MQ=255) gCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATc < 1:463419/69‑1 (MQ=255) gCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGtt < 1:1072164/69‑1 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGtt > 1:2281631/1‑68 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGtt > 1:2453051/1‑68 (MQ=255) cGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGtt > 1:1630892/1‑68 (MQ=255) cGTGGACGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGtt > 1:710634/1‑68 (MQ=255) gTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACg < 1:54321/38‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:3408865/68‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:1651166/68‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:2461879/68‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:1253185/68‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:460537/68‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:933087/68‑1 (MQ=255) tGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTaa < 1:3243847/68‑1 (MQ=255) ggATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATa < 1:2952070/69‑1 (MQ=255) taTGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAc < 1:973623/68‑1 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAc < 1:419245/69‑1 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAc < 1:2950725/69‑1 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAc < 1:2799668/69‑1 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAc < 1:2795693/69‑1 (MQ=255) gATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAc < 1:2201045/69‑1 (MQ=255) tGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATAcc < 1:2363228/68‑1 (MQ=255) gAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATa < 1:511124/68‑1 (MQ=255) gAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATa < 1:1269850/68‑1 (MQ=255) aaaaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATaac < 1:707690/69‑3 (MQ=255) aaaaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATAcc < 1:3217273/69‑1 (MQ=255) aaaaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATAcc < 1:1143851/69‑1 (MQ=255) aaaCCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATAcc < 1:3312329/67‑1 (MQ=255) cGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCa < 1:3115554/69‑1 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAAc < 1:2467522/69‑1 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAAc < 1:2133853/69‑1 (MQ=255) ggTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAAc < 1:1854273/69‑1 (MQ=255) | CACCCATGGTCTGCGTGCTATTTTGCATAACGCCAGTGCGTGGATGGCGGAAAAACCGGGTGCGCCACTGGCCGACGTACTGGCTTGCATGATGCAGATCCACGTTAATACCCCATACCTGCTCAAC > minE/1008891‑1009017 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |