| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,010,065 | T→C | 35.8% | S75S (TCT→TCC) | rstA → | DNA‑bindng response regulator in two‑component regulatory system with RstB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,010,065 | 0 | T | C | 35.8% | 74.5 / 72.5 | 117 | S75S (TCT→TCC) | rstA | DNA‑bindng response regulator in two‑component regulatory system with RstB |
| Reads supporting (aligned to +/- strand): ref base T (53/22); new base C (14/28); total (67/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.75e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 117 total aligned reads displayed.
ATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGA > minE/1010006‑1010124 | atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:223098/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:3150440/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:1789915/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:2099333/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:3278179/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:2598492/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:2584162/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:2536360/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:1670907/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:126792/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:2531812/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:1442834/1‑69 (MQ=255)atcatGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:171330/1‑69 (MQ=255)atcatGCCACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCCGGACCGAtt > 1:2093259/1‑69 (MQ=255) catGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTg < 1:863888/68‑1 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:2209402/1‑69 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:2193371/1‑69 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:30772/1‑69 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:2310060/1‑69 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:1413381/1‑69 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:2433975/1‑69 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:301448/1‑69 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:3204564/1‑69 (MQ=255) atGCTACCAGGCAAGGACGGCATGACCATCTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGtt > 1:710235/1‑69 (MQ=255) gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACct > 1:361058/1‑69 (MQ=255) gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACct > 1:2214950/1‑69 (MQ=255) gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACct > 1:2325051/1‑69 (MQ=255) gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACct > 1:409706/1‑69 (MQ=255) gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACct > 1:100641/1‑69 (MQ=255) gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACct > 1:1283325/1‑69 (MQ=255) gCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACct > 1:777096/1‑69 (MQ=255) gACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCtctctc < 1:3301493/69‑1 (MQ=255) aCCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGat > 1:3315705/1‑69 (MQ=255) aCCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGat > 1:2251963/1‑69 (MQ=255) aCCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGat > 1:76302/1‑69 (MQ=255) aCCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGat > 1:2631569/1‑69 (MQ=255) aCCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGat > 1:1782731/1‑69 (MQ=255) aCCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGat > 1:3289684/1‑69 (MQ=255) aCCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGat > 1:2003840/1‑69 (MQ=255) aCCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGa > 1:312184/1‑68 (MQ=255) tGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:2088387/1‑69 (MQ=255) gTCGTGATTTACGCGGAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:2021081/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTGTTCTAACCTCTCTCGATAGCGATATGAAc > 1:2472866/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGaa > 1:3278602/1‑67 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:964381/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:922728/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:879071/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:1340005/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:275079/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:3411494/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:661161/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:1770395/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:1847033/1‑68 (MQ=255) gTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAAc > 1:460290/1‑68 (MQ=255) cGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCtctctc > 1:801735/1‑51 (MQ=255) cGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCtctctc > 1:1973784/1‑51 (MQ=255) gTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACcaca < 1:622163/69‑1 (MQ=255) gTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACcaca < 1:1563906/69‑1 (MQ=255) gTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACcac < 1:1110978/68‑1 (MQ=255) aTTTACGCGCATAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATca < 1:1071844/69‑2 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:982206/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:3320397/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:3258587/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:976375/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:3365122/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:784333/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:1038717/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:488029/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:2325007/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:1554605/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:1566896/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:1750036/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:1859129/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:1471994/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:1420218/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:2087061/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:117855/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:1150133/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:2745829/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:3032201/69‑1 (MQ=255) aTTTACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:3063601/69‑1 (MQ=255) tttACGCGCAAAGTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:2364879/68‑1 (MQ=255) ttACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCt < 1:886994/68‑1 (MQ=255) ttACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCt < 1:2324625/68‑1 (MQ=255) tACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCt > 1:1206702/1‑67 (MQ=255) tACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTgg > 1:3341671/1‑69 (MQ=255) tACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTgg > 1:2861687/1‑69 (MQ=255) cgcgCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGc < 1:1094779/68‑1 (MQ=255) cgcgCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGc < 1:1113805/68‑1 (MQ=255) cgcgCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGc < 1:1507374/68‑1 (MQ=255) cgcgCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGc < 1:2018995/68‑1 (MQ=255) cgcgCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGc < 1:267447/68‑1 (MQ=255) cgcgCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGc < 1:2965119/68‑1 (MQ=255) cgcgCAAAGTGGGCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGc < 1:2261522/68‑1 (MQ=255) aaaGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGa < 1:2414714/69‑1 (MQ=255) aaGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGa < 1:667310/68‑1 (MQ=255) aaGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGa < 1:188930/68‑1 (MQ=255) aGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGa < 1:2356669/67‑1 (MQ=255) gTGGTCTGGACCGATTGTTCTTCTATCCTCTCTCGATAGCGATATGAACCACATCCt < 1:1195782/57‑1 (MQ=255) gTGGTCCGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATcc < 1:3067319/56‑1 (MQ=255) | ATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGCGCAAAGTGGTCTGGACCGATTGTTCTTCTAACCTCTCTCGATAGCGATATGAACCACATCCTGGCACTGGA > minE/1010006‑1010124 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |