| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,011,495 | T→C | 16.5% | V308A (GTG→GCG) | rstB → | sensory histidine kinase in two‑component regulatory system with RstA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,011,495 | 0 | T | C | 16.5% | 124.9 / 13.6 | 78 | V308A (GTG→GCG) | rstB | sensory histidine kinase in two‑component regulatory system with RstA |
| Reads supporting (aligned to +/- strand): ref base T (41/24); new base C (3/10); total (44/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01 | |||||||||||
TGTCAACGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAAT > minE/1011429‑1011559 | tGTCAACGCATCTGGCAGATATTCAGGCAGTAACTCCCGATAAAACGGTACGGATAAAAACGCTCGt > 1:2296773/1‑67 (MQ=255)tGTCAACGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGt > 1:3151402/1‑67 (MQ=255)tGTCAACGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGt > 1:1617407/1‑67 (MQ=255)tGTCAACGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGt > 1:1517188/1‑67 (MQ=255)tGTCAACGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGc < 1:203768/69‑1 (MQ=255)tGTCAACGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGc < 1:986991/69‑1 (MQ=255)tGTCAACGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGc < 1:2375596/69‑1 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:2017954/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:1641745/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:1632056/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:2331829/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:646632/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:1532656/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:627842/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:2780216/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:491746/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:985740/1‑68 (MQ=255) aaCGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAg > 1:993591/1‑68 (MQ=255) cATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCa > 1:2042750/1‑62 (MQ=255) cATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCAt > 1:282089/1‑69 (MQ=255) cATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCAt > 1:379720/1‑69 (MQ=255) cATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCAt > 1:3209153/1‑69 (MQ=255) cATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCAt > 1:2220570/1‑69 (MQ=255) cATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCAt > 1:130253/1‑69 (MQ=255) cATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCAt > 1:1580998/1‑69 (MQ=255) cTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTAt > 1:1292955/1‑69 (MQ=255) cTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTAt > 1:2673428/1‑69 (MQ=255) cTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTAt > 1:866123/1‑69 (MQ=255) cAGGCAGTAACGCCTGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:296539/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGat > 1:2967706/1‑69 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGat > 1:2980703/1‑69 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGat > 1:1381189/1‑69 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGat > 1:744842/1‑69 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGat > 1:1575672/1‑69 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGat > 1:751651/1‑69 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGat > 1:2393627/1‑69 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGa < 1:1264419/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGa < 1:1287067/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGa < 1:1274053/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGa < 1:849800/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGa < 1:225986/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGa < 1:1751078/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:2384452/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:520287/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:3042967/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:2788712/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:2126103/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:2886068/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:2893155/68‑1 (MQ=255) cAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:1697704/68‑1 (MQ=255) aGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGa < 1:1775959/67‑1 (MQ=255) aGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGCGCAAGGCCATTATGCGGCGTTGGa < 1:2429379/67‑1 (MQ=255) aaCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCTCtt < 1:2713410/69‑1 (MQ=255) aaCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt < 1:3096250/69‑1 (MQ=255) aaCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt < 1:1991432/69‑1 (MQ=255) aaCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt < 1:3317450/69‑1 (MQ=255) aaCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt < 1:2073417/69‑1 (MQ=255) aaCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt < 1:1918264/69‑1 (MQ=255) aCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGa < 1:3199951/59‑1 (MQ=255) aCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt > 1:443775/1‑68 (MQ=255) aCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt > 1:1137315/1‑68 (MQ=255) aCGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt > 1:2896039/1‑68 (MQ=255) gCCCGATAAAACGGTACGGATAAAAACGCTCGTGc < 1:859381/35‑1 (MQ=255) aaaCGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGata < 1:1570394/51‑1 (MQ=255) gTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTg > 1:2959359/1‑69 (MQ=255) gTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTg > 1:1382128/1‑69 (MQ=255) gTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTg > 1:2880910/1‑69 (MQ=255) gTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTg > 1:2365258/1‑69 (MQ=255) gATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATaa > 1:539657/1‑69 (MQ=255) gATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATaa > 1:1214685/1‑69 (MQ=255) gATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATaa > 1:1515031/1‑69 (MQ=255) gATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATaa > 1:2307433/1‑69 (MQ=255) gATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATaa > 1:3432533/1‑69 (MQ=255) aaaaCGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCtt < 1:505354/44‑1 (MQ=255) aaaaCGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAAtt > 1:2450985/1‑67 (MQ=255) aaaaCGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTg > 1:2785049/1‑69 (MQ=255) aaaaCGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTg > 1:3400920/1‑69 (MQ=255) aaaaCGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTg > 1:1154401/1‑69 (MQ=255) aaCGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGc < 1:3020609/68‑1 (MQ=255) cTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAAt < 1:2615857/69‑1 (MQ=255) cTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAAt < 1:3404979/69‑1 (MQ=255) cTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAAt < 1:109783/69‑1 (MQ=255) | TGTCAACGCATCTGGCAGATATTCAGGCAGTAACGCCCGATAAAACGGTACGGATAAAAACGCTCGTGCAAGGCCATTATGCGGCGTTGGATATGCGCTTAATGGAGCGCGTGCTGGATAATTTGCTCAAT > minE/1011429‑1011559 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |