Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,013,728 A→G 37.5% I184I (ATT→ATC fumC ← fumarate hydratase (fumarase C), aerobic Class II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,013,7280AG37.5% 57.0 / 67.6 104I184I (ATT→ATCfumCfumarate hydratase (fumarase C), aerobic Class II
Reads supporting (aligned to +/- strand):  ref base A (27/38);  new base G (11/28);  total (38/66)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

GCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGG  >  minE/1013663‑1013796
                                                                 |                                                                    
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:1758764/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:3173092/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:917133/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:1995670/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:751396/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:1572542/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:3354325/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:44452/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAtt                                                                   >  1:2272968/1‑69 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCGTCCTGCAAGTGGGTACGACCGAt                                                                    >  1:1658082/1‑68 (MQ=255)
gCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGAGTGGCGTCCTGCAAGTGGGTACGACCGAt                                                                    >  1:1817987/1‑68 (MQ=255)
   aCCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTg                                                                >  1:1715599/1‑69 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg                                          <  1:1462262/69‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg                                          <  1:883504/69‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg                                          <  1:2068589/69‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg                                          <  1:3194486/69‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg                                          <  1:2744252/69‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg                                          <  1:1077167/69‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACggg                                          <  1:2922034/69‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACgg                                           <  1:2507121/68‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACgg                                           <  1:3033150/68‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACgg                                           <  1:1757534/68‑1 (MQ=255)
                         ccAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACgg                                           <  1:981060/68‑1 (MQ=255)
                          cAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGa                                         >  1:1275990/1‑69 (MQ=255)
                                       gTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAgt                            <  1:189384/69‑1 (MQ=255)
                                       gTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAgt                            <  1:906722/69‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc                                   <  1:2930459/59‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc                                   <  1:2365110/59‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc                                   <  1:52678/59‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc                                   <  1:1071502/59‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc                                   <  1:2029662/59‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc                                   <  1:863631/59‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc                                   <  1:460810/59‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         <  1:3311301/69‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         >  1:904468/1‑69 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         <  1:2854213/69‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         <  1:2567392/69‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         <  1:151278/69‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         >  1:2124086/1‑69 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         <  1:645921/69‑1 (MQ=255)
                                          gCATCCTGCAAGTGAGTACGACAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCt                        >  1:2640588/1‑69 (MQ=255)
                                          gCATCCTGCAAGTGAGCACGCCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         >  1:1907453/1‑69 (MQ=255)
                                          gCATCCTGCAAGTGAGCACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         >  1:433478/1‑69 (MQ=255)
                                          gCATCCTGCAAGTGAGCACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTc                         >  1:2598783/1‑69 (MQ=255)
                                          gCAACCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTtctc                                   <  1:2927833/59‑1 (MQ=255)
                                              ccTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCtgt                      >  1:498804/1‑68 (MQ=255)
                                               cTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCtgt                      >  1:3047722/1‑67 (MQ=255)
                                               cTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCtgt                      >  1:724040/1‑67 (MQ=255)
                                                    aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg               <  1:2514125/69‑1 (MQ=255)
                                                    aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg               <  1:142525/69‑1 (MQ=255)
                                                    aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg               <  1:3367781/69‑1 (MQ=255)
                                                    aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg               <  1:895411/69‑1 (MQ=255)
                                                    aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg               <  1:1616141/69‑1 (MQ=255)
                                                    aGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAggg               <  1:1634908/69‑1 (MQ=255)
                                                       gAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCa                                  >  1:725928/1‑47 (MQ=255)
                                                       gAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCa                                  >  1:1131390/1‑47 (MQ=255)
                                                       gAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCa                                  >  1:2309467/1‑47 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:3273517/68‑1 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:685600/68‑1 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:712477/68‑1 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:102915/68‑1 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:1913001/68‑1 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:1824313/68‑1 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:1584478/68‑1 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:2374297/68‑1 (MQ=255)
                                                        ggTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:180309/68‑1 (MQ=255)
                                                        ggTAAGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:2192778/68‑1 (MQ=255)
                                                         gTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:1332287/68‑1 (MQ=255)
                                                         gTACGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCTGGGTCAGGGtttt           <  1:34563/68‑1 (MQ=255)
                                                          tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTaa         >  1:1575443/1‑69 (MQ=255)
                                                          tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTaa         >  1:877559/1‑69 (MQ=255)
                                                          tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTaa         >  1:2525057/1‑69 (MQ=255)
                                                          tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTaa         >  1:862417/1‑69 (MQ=255)
                                                          tACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTAAGTGTCTGTGTCAGGGTTTTaa         >  1:2915390/1‑69 (MQ=255)
                                                            cGACCGATTTTGACGATATCTGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:361852/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTGAGCGTCt                        <  1:3093303/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:1672772/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:1688926/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:789242/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:926929/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:2903447/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:1933544/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:2266118/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:2108796/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:2855500/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:362728/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:278332/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:2329545/52‑1 (MQ=255)
                                                            cGACCGATTTTGACGATATCGGCAAATGCGCGGGATTTTTCACTCAGCGTCt                        <  1:250776/52‑1 (MQ=255)
                                                            cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg        >  1:1002638/1‑68 (MQ=255)
                                                            cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg        >  1:1505039/1‑68 (MQ=255)
                                                            cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg        >  1:2759052/1‑68 (MQ=255)
                                                            cGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg        >  1:257066/1‑68 (MQ=255)
                                                            cGACCAATTTTGACGATATCGGCAAAACCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAg        >  1:139029/1‑68 (MQ=255)
                                                              aCCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCt      <  1:375053/68‑1 (MQ=255)
                                                                 aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg  <  1:1603658/69‑1 (MQ=255)
                                                                 aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg  <  1:362549/69‑1 (MQ=255)
                                                                 aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg  <  1:3038794/69‑1 (MQ=255)
                                                                 aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg  <  1:1188023/69‑1 (MQ=255)
                                                                 aaTTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAgg  <  1:2272089/69‑1 (MQ=255)
                                                                 |                                                                    
GCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGGCAAAAGCACGGGATTTCTCATTCAGTGTCTGTGTCAGGGTTTTAAGCTGAGG  >  minE/1013663‑1013796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: