| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 89,165 | A→G | 14.1% | G148G (GGA→GGG) | ftsA → | ATP‑binding cell division protein involved in recruitment of FtsK to Z ring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 89,165 | 0 | A | G | 14.1% | 344.8 / 33.4 | 196 | G148G (GGA→GGG) | ftsA | ATP‑binding cell division protein involved in recruitment of FtsK to Z ring |
| Reads supporting (aligned to +/- strand): ref base A (88/80); new base G (9/19); total (97/99) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01 | |||||||||||
Only 100 of 197 total aligned reads displayed.
GTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAA > minE/89098‑89230 | gtgtGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAc > 1:960498/1‑69 (MQ=255)gtgtGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGAc > 1:2900119/1‑69 (MQ=255) tgATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTg > 1:3323842/1‑68 (MQ=255) tgATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTg > 1:1570142/1‑68 (MQ=255) tgATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTg > 1:626653/1‑68 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:2224210/68‑1 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:251818/68‑1 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:2696441/68‑1 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:288312/68‑1 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:2926583/68‑1 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:3118558/68‑1 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:336309/68‑1 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:835837/68‑1 (MQ=255) aTCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCg < 1:1195745/68‑1 (MQ=255) gTATGCGATTGACTATCAGGACGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaaa < 1:1454895/69‑1 (MQ=255) gTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaaa < 1:2677865/69‑1 (MQ=255) aTGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg < 1:474339/69‑1 (MQ=255) aTGCGATTGACTATCAGGAAGAGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg < 1:675530/69‑1 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:513220/1‑68 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg < 1:2626108/68‑1 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:1089525/1‑68 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:105926/1‑68 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:1129169/1‑68 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:849361/1‑68 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:1933552/1‑68 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:1226781/1‑68 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:339657/1‑68 (MQ=255) tGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg > 1:1563131/1‑68 (MQ=255) cGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACt < 1:1212406/40‑1 (MQ=255) cGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACt > 1:453152/1‑40 (MQ=255) cGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACt > 1:463868/1‑40 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg < 1:515636/69‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg < 1:2471252/69‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg < 1:1025521/69‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg < 1:2712725/69‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg < 1:2815705/69‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg < 1:3049187/69‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTg < 1:1426037/69‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaa < 1:869926/58‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaa < 1:23281/58‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaa > 1:1880673/1‑58 (MQ=255) gACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaa < 1:2194620/58‑1 (MQ=255) gACTATCAGGAAGGGATCAAGAATACGGTAGGACTTTCGGGCGTGCGGATGCAGGCaa > 1:403841/1‑58 (MQ=255) cTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGa < 1:1296594/68‑1 (MQ=255) cTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGa < 1:1311497/68‑1 (MQ=255) cTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGa < 1:3097147/68‑1 (MQ=255) cTATCAGGAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGa < 1:3059716/68‑1 (MQ=255) tATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGAt < 1:1019079/68‑1 (MQ=255) tATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGAt < 1:2578048/68‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:2606689/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:516494/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:1529327/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:24413/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:3334031/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:3247167/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:3213617/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:3176435/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:3133259/69‑1 (MQ=255) aTCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca < 1:2150248/69‑1 (MQ=255) aGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcaca < 1:2017883/68‑1 (MQ=255) aGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcaca < 1:3166212/68‑1 (MQ=255) aGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcaca < 1:2208539/68‑1 (MQ=255) ggAAGGGATCAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGa < 1:2609321/62‑1 (MQ=255) aGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTc > 1:225888/1‑68 (MQ=255) aGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTc > 1:1595463/1‑68 (MQ=255) aGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTc > 1:2426228/1‑68 (MQ=255) aGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTc > 1:561455/1‑68 (MQ=255) aGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTc > 1:786209/1‑68 (MQ=255) aGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTc > 1:1176839/1‑68 (MQ=255) aGGGAT‑AAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTcac > 1:2286131/1‑69 (MQ=255) cAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcac > 1:593164/1‑57 (MQ=255) cAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATcac > 1:897279/1‑57 (MQ=255) cAAGAATCCGGTAGGGCTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATca > 1:3039387/1‑56 (MQ=255) cAAGAATCCGGTAGGACTTTCGTGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa > 1:1573112/1‑69 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTGACAACGa > 1:325076/1‑69 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa > 1:743216/1‑69 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:135849/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa > 1:2347354/1‑69 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:2522314/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:2855622/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa > 1:419127/1‑69 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa > 1:3428710/1‑69 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:2025251/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:2914149/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa > 1:1912466/1‑69 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:3016772/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:2773166/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:2747936/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa > 1:1789367/1‑69 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:1737063/69‑1 (MQ=255) cAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGa < 1:2637483/69‑1 (MQ=255) aaGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAg < 1:2196683/43‑1 (MQ=255) gAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCaa < 1:204717/38‑1 (MQ=255) aGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa > 1:2090412/1‑69 (MQ=255) aGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa > 1:1380940/1‑69 (MQ=255) aGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa > 1:2138087/1‑69 (MQ=255) aGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa > 1:2492186/1‑69 (MQ=255) aGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa > 1:2382558/1‑69 (MQ=255) aGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaaa > 1:1713068/1‑69 (MQ=255) aGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGaaaa > 1:1894221/1‑68 (MQ=255) | GTGTGCTGCATGTGATCCCGCAAGAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTGCACCTGATCACATGTCACAACGATATGGCGAAAAA > minE/89098‑89230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |