| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,889 | G→A | 63.3% | F60F (TTC→TTT) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,889 | 0 | G | A | 63.3% | 25.2 / 28.3 | 49 | F60F (TTC→TTT) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (3/15); new base A (25/6); total (28/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.72e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCT > minE/1015835‑1015956 | cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGCCGCAGCATAAATGACGcatcat > 1:1689740/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:1117182/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:2594463/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:831154/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:811869/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:2258868/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:2230705/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:476474/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:2091616/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:2024521/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:1967066/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:1958393/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:1949422/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:526216/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:553278/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:58768/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:598121/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:1756296/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:1734874/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:615232/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:139194/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:1195511/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCCGACGCAGCATAAATGACGcatcat > 1:2193735/1‑68 (MQ=255)cTCCGGGTCACGCAGAATGTCGGCCACCCGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:1896681/1‑68 (MQ=255)cTCCGGGTCAAGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGcatcat > 1:726123/1‑68 (MQ=255) tCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:3034604/69‑1 (MQ=255) tCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:239805/69‑1 (MQ=255) tCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:2745310/69‑1 (MQ=255) tCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:260649/69‑1 (MQ=255) tCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:1694871/69‑1 (MQ=255) tCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGAAGCAGCATGAACGACGCATCATGa < 1:1941152/69‑1 (MQ=255) tCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGa < 1:1943248/63‑1 (MQ=255) cACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGCATCATGAAACGCCt < 1:1665199/69‑1 (MQ=255) cACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGCATCATGAAACGCCt < 1:2588783/69‑1 (MQ=255) cACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGCATCATGAAACGCCt < 1:1797962/69‑1 (MQ=255) cACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGCATCATGAAACGCCt < 1:1060286/69‑1 (MQ=255) cACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGCATCATGAAACGCCt < 1:3352350/69‑1 (MQ=255) cACGCAGAATGTCGGCCACCTGTTGTTGGTGAGCCGGACGCAGCATAAATGACGCATCATGAAACGCCt < 1:1837099/69‑1 (MQ=255) gttgGTGCGCCGGACGCAGCATGAACGACGCATCATGa > 1:2774131/1‑38 (MQ=255) gttgGTGCGCCGGACGCAGCATGAACGACGCATCATGa > 1:801532/1‑38 (MQ=255) gttgGTGCGCCGGACGCAGCATGAACGACGCATCATGa > 1:2966635/1‑38 (MQ=255) ccGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCg < 1:2159108/68‑1 (MQ=255) agcaTGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCaaa < 1:3037983/69‑1 (MQ=255) agcaTGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCaaa < 1:2818373/69‑1 (MQ=255) gcaTGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCaaa < 1:3150348/68‑1 (MQ=255) tGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtct < 1:866815/69‑1 (MQ=255) tGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtct < 1:472466/69‑1 (MQ=255) tGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtct < 1:3200007/69‑1 (MQ=255) tGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAAtct < 1:2403826/69‑1 (MQ=255) | CTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCT > minE/1015835‑1015956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |