| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,017,320 | T→C | 100% | L352L (TTG→CTG) | manA → | mannose‑6‑phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,017,320 | 0 | T | C | 80.5% | 106.7 / 15.5 | 71 | L352L (TTG→CTG) | manA | mannose‑6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (14/0); new base C (41/16); total (55/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.95e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGAATCAGCGTTTATTGCCGCCAA > minE/1017258‑1017381 | aaGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGGGTCGAAGGCGATGCAACGTtgtg > 1:792856/1‑67 (MQ=255)aaGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTtgtg > 1:3027753/1‑67 (MQ=255)aaGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTtgtg > 1:2709647/1‑67 (MQ=255)aaGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTtgtg > 1:1895492/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCTCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:3185909/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:1114126/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:671625/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:3147641/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:3010770/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:2821634/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:274915/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:2628497/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:2290361/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:2198716/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:2141680/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:2065535/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:1086144/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGtt > 1:1462394/1‑67 (MQ=255) accaTCAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGt > 1:2568952/1‑66 (MQ=255) ttAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTcagc > 1:301578/1‑69 (MQ=255) ttAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTcagc > 1:3039201/1‑69 (MQ=255) ttAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTcagc > 1:2312722/1‑69 (MQ=255) ttAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTcagc > 1:378741/1‑69 (MQ=255) ttAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTcagc > 1:2045819/1‑69 (MQ=255) ttAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTcagc > 1:1659852/1‑69 (MQ=255) ttAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTcag > 1:1488524/1‑68 (MQ=255) ttAGCCAGCAGAGTGCCGCCATTTCGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTcagc > 1:1319895/1‑69 (MQ=255) gccATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTCAGCAGTTACATCTTAAACCg > 1:3413459/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTa > 1:2311150/1‑59 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTg > 1:285073/1‑68 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTg > 1:1936795/1‑68 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTg > 1:2252072/1‑68 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTg > 1:1115681/1‑68 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTg > 1:234609/1‑68 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:3153135/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1371857/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1232774/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:3122922/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:3086596/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:689961/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:553551/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:1169424/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:3329352/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:3342622/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:3431737/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:591498/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:405142/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:471526/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:587199/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:2955101/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1572901/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:1643585/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1728747/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1524096/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:1941737/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:2028725/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:1503532/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:2086501/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:2124365/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:2221328/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:2354427/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:2611612/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1391412/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:2634463/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:2654644/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa < 1:1545209/69‑1 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1346987/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1341095/1‑69 (MQ=255) tttGTTCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGCTGa > 1:1412216/1‑69 (MQ=255) tttGTCCTGCGTCGAAGGCGATGCAACGCTGTGTAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGa > 1:1362445/1‑69 (MQ=255) gCAACGTTGTGGAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGAATCAGCGTTTATTGCCGCCaa > 1:2796289/1‑68 (MQ=255) | AAGAAACCACCATTAGCCAGCAGAGTGCCGCCATTTTGTTCTGCGTCGAAGGCGATGCAACGTTGTGGAAAGGTTCTCAGCAGTTACAGCTTAAACCGGGTGAATCAGCGTTTATTGCCGCCAA > minE/1017258‑1017381 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |