| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 90,611 | G→A | 41.6% | L189L (CTG→CTA) | ftsZ → | GTP‑binding tubulin‑like cell division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 90,611 | 0 | G | A | 41.6% | 43.9 / 78.1 | 108 | L189L (CTG→CTA) | ftsZ | GTP‑binding tubulin‑like cell division protein |
| Reads supporting (aligned to +/- strand): ref base G (40/23); new base A (31/14); total (71/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
Only 100 of 110 total aligned reads displayed.
AACTGCTGAAAGTTCTGGGCCGCGGTATCTCCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGA > minE/90544‑90679 | aaCTGCTGAAAGTTCTGGGCAGCGGTATCTCCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGa < 1:2897266/69‑1 (MQ=255) tCTGGGCCTCGGTATCTCCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCa < 1:345788/69‑1 (MQ=255) tCTGGGCCGCGGTATCTCCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCa < 1:2445858/69‑1 (MQ=255) tctcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGaa > 1:3420211/1‑68 (MQ=255) tcactgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAAc > 1:1252950/4‑67 (MQ=255) tcCCTGCTGGATGCTTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2914477/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAGCGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:1968314/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGaa > 1:1172040/1‑66 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACt > 1:72648/1‑68 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACt > 1:1713344/1‑68 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACt > 1:1947268/1‑68 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACt > 1:1318696/1‑68 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:3195691/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2037005/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2401500/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2135346/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2114648/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2046722/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2012996/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2621638/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:3039121/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2627451/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2853112/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2895311/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1862982/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1829650/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:678187/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1324651/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:551994/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1292041/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:911289/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1201638/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1402729/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:95353/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1623049/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1851884/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:2951743/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:127734/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:308942/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:3144389/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:3183360/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:971688/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:3205864/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:2303115/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:2064840/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:3241054/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:523370/68‑1 (MQ=255) tcCCAGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:3204253/68‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:1903064/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2380702/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:3395721/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:922328/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:372729/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2674328/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:3290774/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:257119/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2269012/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2474928/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:732512/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2380847/67‑1 (MQ=255) gctgTATGCGTTTGGCGCATCGAACGATTTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2472157/66‑1 (MQ=255) gCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCg > 1:256327/1‑69 (MQ=255) gCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCg > 1:1320325/1‑69 (MQ=255) gCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCg > 1:2348390/1‑69 (MQ=255) cAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTtgatga < 1:2797491/69‑1 (MQ=255) cAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTtgatga < 1:1147882/69‑1 (MQ=255) cAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTtgatga < 1:1355734/69‑1 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTatgatg > 1:1368363/1‑68 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:559510/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1591915/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1589846/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:146815/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1400184/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:379873/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:410737/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3304389/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:568805/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1353335/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1319896/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1287913/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1191638/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1135229/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2896474/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2913644/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2622488/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3052854/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3054147/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2617934/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3140890/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3155773/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:24696/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:235902/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2198077/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3263010/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2094147/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3316830/1‑69 (MQ=255) cGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:1069289/46‑1 (MQ=255) cGATGTACTGAAATGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2533245/42‑1 (MQ=255) gAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGa < 1:2963860/69‑1 (MQ=255) gAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGa < 1:2746582/69‑1 (MQ=255) | AACTGCTGAAAGTTCTGGGCCGCGGTATCTCCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGA > minE/90544‑90679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |