| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,020,036 | A→G | 57.2% | D169D (GAT→GAC) | uidC ← | predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,020,036 | 0 | A | G | 57.2% | 24.6 / 56.5 | 77 | D169D (GAT→GAC) | uidC | predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base A (21/12); new base G (15/29); total (36/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.23e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
CATCACGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTA > minE/1019972‑1020096 | catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:2162171/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:1719023/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:2926781/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:2930691/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:2942201/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:2898120/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:2833597/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:3260714/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCaa > 1:2691027/1‑69 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCa > 1:609018/1‑68 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCa > 1:473359/1‑68 (MQ=255)catcaCGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCa > 1:2467028/1‑68 (MQ=255) acaggTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGgtg > 1:2486418/4‑68 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCTCCACAGGTCAATATTTGGCGTTaa < 1:1195260/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:2750992/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:2458351/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:2301708/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:2964942/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:996483/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:3009187/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:460872/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:611568/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:670095/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:74368/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:744467/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:82666/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:839597/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:1362679/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:1901670/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:1340565/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:1890363/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:2001764/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:1285253/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:1634478/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:1171178/69‑1 (MQ=255) ggTTGTTGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:1822226/69‑1 (MQ=255) ggTTGTT‑AATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:2091030/68‑1 (MQ=255) gttgttGAATCGGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:975180/68‑1 (MQ=255) gttgAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGc > 1:1025357/1‑68 (MQ=255) tgAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGccc < 1:2859776/68‑1 (MQ=255) tgAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGccc < 1:2947389/68‑1 (MQ=255) aGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAAc > 1:1324688/1‑68 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTaa < 1:3160055/53‑1 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAAt > 1:1045692/1‑69 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAAt > 1:57442/1‑69 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAAt > 1:2810701/1‑69 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAAt > 1:1721226/1‑69 (MQ=255) cTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCa > 1:839718/1‑68 (MQ=255) cTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCa > 1:2366328/1‑68 (MQ=255) gTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTaa < 1:184018/55‑1 (MQ=255) gTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTaa < 1:1859870/55‑1 (MQ=255) gTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTaa < 1:2445616/55‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:1509602/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:580172/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:915501/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:704601/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:816/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:109325/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:1824142/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:1565901/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:2685249/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:1747893/69‑1 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCt > 1:3366341/1‑48 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1613192/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:2038235/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:2038991/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:962575/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:2245930/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:2417925/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1784359/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:686073/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1292686/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1554046/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:3146949/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1503713/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:487356/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTACTTCAGAAACTGTCgg > 1:1503286/1‑62 (MQ=255) | CATCACGAATACCGGTTGTTGAATCAGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTA > minE/1019972‑1020096 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |