| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,020,061 | T→C | 37.5% | Y161C (TAT→TGT) | uidC ← | predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,020,061 | 0 | T | C | 37.5% | 23.1 / 28.4 | 48 | Y161C (TAT→TGT) | uidC | predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base T (9/21); new base C (15/3); total (24/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.82e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.76e-01 | |||||||||||
AGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACGGAATACGA > minE/1019997‑1020129 | aGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAAc > 1:1324688/1‑68 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAAt > 1:1045692/1‑69 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAAt > 1:2810701/1‑69 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAAt > 1:57442/1‑69 (MQ=255) cATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAAt > 1:1721226/1‑69 (MQ=255) cTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCa > 1:2366328/1‑68 (MQ=255) cTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCa > 1:839718/1‑68 (MQ=255) gTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTaa < 1:2445616/55‑1 (MQ=255) gTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTaa < 1:1859870/55‑1 (MQ=255) gTGAACGCCACATTCCCCACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTaa < 1:184018/55‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:1747893/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:2685249/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:1824142/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:580172/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:1565901/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:1509602/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:704601/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:816/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:915501/69‑1 (MQ=255) ttCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATg < 1:109325/69‑1 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCt > 1:3366341/1‑48 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:962575/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:487356/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:686073/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:3146949/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:2417925/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:2245930/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1292686/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:2038991/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1503713/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:2038235/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1554046/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1613192/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTa > 1:1784359/1‑69 (MQ=255) ccccACAGGTCAATATTTGGCGTTAAAGCCCAACAACCTAATGCTACTTCAGAAACTGTCgg > 1:1503286/1‑62 (MQ=255) tGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTgccagc < 1:880921/69‑1 (MQ=255) tGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTgccagc < 1:1757285/69‑1 (MQ=255) aaaGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTaa < 1:2202861/68‑1 (MQ=255) aaaGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTaa < 1:807776/68‑1 (MQ=255) aaaGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTaa < 1:3020599/68‑1 (MQ=255) aaaGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTaa < 1:2706933/68‑1 (MQ=255) aaaGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTaa < 1:2649903/68‑1 (MQ=255) aaGCCCAATAACCTAATGCTCCTTCAGAAACTGTCTGTAATGTACGTTGCGAACTGCCAGCCATTaa < 1:2496343/67‑1 (MQ=255) gCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACgg < 1:1921553/68‑1 (MQ=255) gCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACgg < 1:2119668/68‑1 (MQ=255) cccAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACGGaa < 1:1908214/69‑1 (MQ=255) ccAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACGGAAt > 1:1428922/1‑69 (MQ=255) ccAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACGGAAt > 1:1424644/1‑69 (MQ=255) tAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACGGAATACGa < 1:311190/69‑1 (MQ=255) tAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACGGAATACGa < 1:2420410/69‑1 (MQ=255) | AGTCCATAAAAATACTCGTGAACGCCACATTCCCCACAGATCAATATTTGGTGTTAAAGCCCAATAACCTAATGCTCCTTCAGAAACTGTCGGTAATGTACGTTGCGAACTGCCAGCCATTAACGGAATACGA > minE/1019997‑1020129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |