| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,020,261 | A→G | 30.9% | S94S (AGT→AGC) | uidC ← | predicted outer membrane porin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,020,261 | 0 | A | G | 30.9% | 48.7 / 26.0 | 58 | S94S (AGT→AGC) | uidC | predicted outer membrane porin protein |
| Reads supporting (aligned to +/- strand): ref base A (25/15); new base G (9/9); total (34/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCAATAAT > minE/1020195‑1020323 | gggTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACt > 1:985651/1‑69 (MQ=255)gggTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACt > 1:1160408/1‑69 (MQ=255)gggTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACt > 1:2033096/1‑69 (MQ=255)gggTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACt > 1:16896/1‑69 (MQ=255)gggTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACt > 1:1365413/1‑69 (MQ=255)gggTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACt > 1:297705/1‑69 (MQ=255) ttttATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCat < 1:2130253/69‑1 (MQ=255) tATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATc > 1:3183658/1‑69 (MQ=255) tATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATc > 1:2201451/1‑69 (MQ=255) tATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATc > 1:1614621/1‑69 (MQ=255) tATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATc > 1:738760/1‑69 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCatc > 1:82960/1‑66 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAGc > 1:1432956/1‑68 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAGc > 1:2380314/1‑68 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAGc > 1:2588953/1‑68 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAGc > 1:1573133/1‑68 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAGc > 1:2116953/1‑68 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAGc > 1:3240520/1‑68 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAGc > 1:1934397/1‑68 (MQ=255) tttACTGCCCCCAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAGc > 1:2861695/1‑68 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:2256537/57‑1 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:1154719/57‑1 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:2608729/57‑1 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:2841783/57‑1 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:1892261/57‑1 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:1565768/57‑1 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:1522804/57‑1 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:318584/57‑1 (MQ=255) ccAGGGTAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTGCTCATATCAg < 1:1453888/57‑1 (MQ=255) gCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta < 1:726813/69‑1 (MQ=255) gCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta < 1:1125977/69‑1 (MQ=255) gCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta < 1:869918/69‑1 (MQ=255) gCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta < 1:1382282/69‑1 (MQ=255) cAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta > 1:2193722/1‑68 (MQ=255) cAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta > 1:2125344/1‑68 (MQ=255) cAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta > 1:1305486/1‑68 (MQ=255) cAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta > 1:1370217/1‑68 (MQ=255) cAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTta > 1:1948810/1‑68 (MQ=255) cAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACat < 1:2201732/69‑1 (MQ=255) cAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACat < 1:1053518/69‑1 (MQ=255) cAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACat < 1:887314/69‑1 (MQ=255) cAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACat < 1:534259/69‑1 (MQ=255) cAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACat < 1:1604207/69‑1 (MQ=255) cAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACat < 1:2493959/69‑1 (MQ=255) aTCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTc < 1:1274752/69‑1 (MQ=255) aTCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAAAATAATACGCCCCGCCTTc < 1:1974320/69‑1 (MQ=255) aaGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAAc > 1:123821/1‑69 (MQ=255) accaccGGGTACTCATATCAGCGCGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGcc > 1:1607277/1‑67 (MQ=255) accaccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGcc > 1:303955/1‑67 (MQ=255) accaccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGcc > 1:1504400/1‑67 (MQ=255) accaccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCa > 1:2932684/1‑68 (MQ=255) accaccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCa > 1:2633659/1‑68 (MQ=255) accaccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCa > 1:88751/1‑68 (MQ=255) accGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCaataa > 1:2773831/1‑69 (MQ=255) accGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCaataa > 1:208491/1‑69 (MQ=255) accGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCaataa > 1:841065/1‑69 (MQ=255) ccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCaataat < 1:3166426/69‑1 (MQ=255) ccGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCaataat < 1:2213568/69‑1 (MQ=255) | GGGTTTTATTTTTACTGCCCCCAGGGCAAAGCCAAAACTTTTATCACCATCAAGATACCACCGGGTACTCATATCAGCACGAGCACCTAATTTATAAACATAATACGCCCCGCCTTCAACGCCAATAAT > minE/1020195‑1020323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |