| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,025,024 | C→G | 46.7% | A236A (GCG→GCC) | hdhA ← | 7alpha‑hydroxysteroid dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,025,024 | 0 | C | G | 46.7% | 4.6 / 58.7 | 53 | A236A (GCG→GCC) | hdhA | 7alpha‑hydroxysteroid dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (9/19); new base G (15/9); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
AGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGAC > minE/1024958‑1025082 | aGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgc < 1:784974/69‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTTGctgcag < 1:1581116/68‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:2650785/68‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:3302101/68‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:2863363/68‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:2565847/68‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:260601/68‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:15208/68‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:1075510/68‑1 (MQ=255) ttAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:876302/68‑1 (MQ=255) tAATTGAGTTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGctgcag < 1:1202243/67‑1 (MQ=255) tAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgc < 1:822168/62‑1 (MQ=255) tAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgc < 1:2819699/62‑1 (MQ=255) tAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgc < 1:2261124/62‑1 (MQ=255) tCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg < 1:218867/69‑1 (MQ=255) tCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg < 1:2335592/69‑1 (MQ=255) ccTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg > 1:2689948/1‑68 (MQ=255) ccTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg > 1:1103597/1‑68 (MQ=255) ccTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg > 1:90948/1‑68 (MQ=255) ccTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg > 1:3060182/1‑68 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:386818/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:3248400/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:3400042/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:2627573/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:499798/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:693183/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:861436/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:985318/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:101268/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:1034961/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:1050116/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:1118688/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:1937497/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:2289885/1‑61 (MQ=255) tGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTGGCTGCAGGCGAGc > 1:2009897/1‑61 (MQ=255) accGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCgcagca > 1:1027652/1‑43 (MQ=255) accGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCa > 1:2871288/1‑51 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:3036313/69‑1 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:2648893/69‑1 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:2443755/69‑1 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:2339461/69‑1 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:1404128/69‑1 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:3245010/69‑1 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:1954363/69‑1 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:1975976/69‑1 (MQ=255) gaCGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAat < 1:3093634/69‑1 (MQ=255) tGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg > 1:624216/1‑36 (MQ=255) tGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg > 1:815174/1‑36 (MQ=255) tGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAgg > 1:2264893/1‑36 (MQ=255) tttcccAGCGCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGAc < 1:419344/66‑1 (MQ=255) ttACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGAc < 1:1993248/69‑1 (MQ=255) ttACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGAc < 1:939049/69‑1 (MQ=255) ttACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTCCGGTTGGCCCAGAc < 1:127514/69‑1 (MQ=255) | AGTGTATTAATTGAGCTCCTGTACCCCACCACCGGAGACGGTGAGAATTTGTCCGCTTACCCAGCTCGCAGCAGGCGAGCAAAGGAACAGCGCTGCGTTAGCAATATCTTGCGGTTGGCCCAGAC > minE/1024958‑1025082 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |