| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 90,635 | T→C | 36.3% | A197A (GCT→GCC) | ftsZ → | GTP‑binding tubulin‑like cell division protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 90,635 | 0 | T | C | 36.3% | 105.1 / 105.6 | 165 | A197A (GCT→GCC) | ftsZ | GTP‑binding tubulin‑like cell division protein |
| Reads supporting (aligned to +/- strand): ref base T (53/52); new base C (46/14); total (99/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.79e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
Only 100 of 165 total aligned reads displayed.
TCTCCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGA > minE/90571‑90700 | tcactgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAAc > 1:1252950/4‑67 (MQ=255) tcCCTGCTGGATGCTTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2914477/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAGCGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:1968314/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGaa > 1:1172040/1‑66 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACt > 1:1713344/1‑68 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACt > 1:72648/1‑68 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1324651/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1829650/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1862982/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1623049/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2012996/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:3195691/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2046722/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1402729/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2114648/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:546840/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2135346/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:551994/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:1292227/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2895311/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:95353/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2853112/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTg > 1:2627451/1‑69 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:971688/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:3183360/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:2064840/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:3144389/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:3205864/68‑1 (MQ=255) tcCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACt < 1:308942/68‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:3290774/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:922328/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2380702/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2674328/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:257119/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:372729/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:1565018/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:3395721/67‑1 (MQ=255) tgctgGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:1903064/67‑1 (MQ=255) gctgTATGCGTTTGGCGCATCGAACGATTTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2472157/66‑1 (MQ=255) gCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACt > 1:3066910/1‑63 (MQ=255) gCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCg > 1:256327/1‑69 (MQ=255) cAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTtgatga < 1:2797491/69‑1 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTatgatg > 1:1368363/1‑68 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2617934/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:24696/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3263010/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1135229/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2622488/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2316404/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2896474/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1191638/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3052854/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:568805/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1287913/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1319896/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1353335/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:379873/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:146815/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1589846/1‑69 (MQ=255) cAGCGAACGATGTACTAAAAGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:3304389/1‑69 (MQ=255) cGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:1069289/46‑1 (MQ=255) cGATGTACTGAAATGCGCTGTGCAAGGTATCGCTGAACTGAt < 1:2533245/42‑1 (MQ=255) gAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGa < 1:2963860/69‑1 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2195509/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2695980/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1202016/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:720212/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:716324/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1306073/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:509697/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:229800/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:1468495/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:159332/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGGTTtgatga > 1:2227727/1‑50 (MQ=255) aGGCGCTGTGCAAGGTATCGCCGAACTGATTACTCGTCCGGCTTtgatga > 1:2042523/1‑50 (MQ=255) gcTGTGCAAGTTATCGCTGAACTGATTACTCGTCCGGGTTtgatg < 1:2189773/45‑1 (MQ=255) gcTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTtgatg < 1:1059188/45‑1 (MQ=255) gcTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTtgatg < 1:1986832/45‑1 (MQ=255) gcTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTtgatg < 1:3252981/45‑1 (MQ=255) gcTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTtgatg < 1:2262891/45‑1 (MQ=255) cTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCa < 1:1191097/69‑1 (MQ=255) cTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCa < 1:836157/69‑1 (MQ=255) cAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTa > 1:236883/1‑69 (MQ=255) cAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTa > 1:2350321/1‑69 (MQ=255) aTCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTg > 1:2092826/1‑46 (MQ=255) aTCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTg < 1:1814658/46‑1 (MQ=255) aTCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTg > 1:948565/1‑46 (MQ=255) tCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGt > 1:2394031/1‑66 (MQ=255) tCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGt > 1:1147577/1‑66 (MQ=255) tCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGt > 1:1252267/1‑66 (MQ=255) tCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGt > 1:1325434/1‑66 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:2318799/68‑1 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:2789990/68‑1 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:2741195/68‑1 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:781639/68‑1 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:1396535/68‑1 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:2986345/68‑1 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:1700916/68‑1 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:1721909/68‑1 (MQ=255) gCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTga < 1:2242756/68‑1 (MQ=255) | TCTCCCTGCTGGATGCGTTTGGCGCAGCGAACGATGTACTGAAAGGCGCTGTGCAAGGTATCGCTGAACTGATTACTCGTCCGGGTTTGATGAACGTGGACTTTGCAGACGTACGCACCGTAATGTCTGA > minE/90571‑90700 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |