| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,025,485 | T→C | 40.9% | I83V (ATC→GTC) | hdhA ← | 7alpha‑hydroxysteroid dehydrogenase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,025,485 | 0 | T | C | 40.9% | 21.8 / 46.7 | 66 | I83V (ATC→GTC) | hdhA | 7alpha‑hydroxysteroid dehydrogenase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (26/13); new base C (17/10); total (43/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAAACGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACGGCAGGCA > minE/1025418‑1025543 | tCAAACGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGAt > 1:565083/1‑68 (MQ=255)tCAAACGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGAt > 1:2049433/1‑68 (MQ=255)tCAAACGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGAt > 1:3019954/1‑68 (MQ=255)tCAAACGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGAt > 1:709438/1‑68 (MQ=255)tCAAACGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGAt > 1:50738/1‑68 (MQ=255)tCAAACGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGAt > 1:2222578/1‑68 (MQ=255)tCAAACGGTTTAGGTCCACAGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGAt > 1:1403031/1‑68 (MQ=255) aaaCGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGATAGc < 1:1220791/69‑1 (MQ=255) aaaCGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGATAGc < 1:2707865/69‑1 (MQ=255) aaCGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGATAGc < 1:628410/68‑1 (MQ=255) ccaccgccaccgGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAg > 1:2848682/1‑68 (MQ=255) ccaccgccaccgGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAg > 1:2737798/1‑68 (MQ=255) ccaccgccaccgGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAg > 1:2695353/1‑68 (MQ=255) accgGCGTTGTTAACCAGGATATCAACTTTACCCAACTTACTGACGGCAAAGTCTGCCAGTGCagagag < 1:1194372/69‑1 (MQ=37) accgGCGTTGTTAACCAGGATATCAACTTTACCCAACTTACTGACGGCAAAGTCTGCCAGTGCagagag < 1:1769435/69‑1 (MQ=37) gttAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGtt > 1:3002181/1‑68 (MQ=255) gttAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGtt > 1:2165210/1‑68 (MQ=255) gttAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGtt > 1:1913017/1‑68 (MQ=255) gttAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGtt > 1:1881633/1‑68 (MQ=255) gttAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGtt > 1:1169616/1‑68 (MQ=255) aaCCAGGATATCAACTTTACCCAACTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCgg < 1:195489/68‑1 (MQ=255) aaCCAGGATATCAACTTTACCCAACTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCgg < 1:878867/68‑1 (MQ=255) aaCCAGGATATCAACTTTACCCAACTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCgg < 1:3106333/68‑1 (MQ=255) aaCCAGGATATCAACTTTACCCAACTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCgg < 1:3251982/68‑1 (MQ=255) aaCCAGGATATCAACTTTACCCAACTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCgg < 1:2866312/68‑1 (MQ=255) aaCCAGGATATCAACTTTACCCAACTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCgg < 1:3425196/68‑1 (MQ=255) gAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTaa > 1:2227201/1‑69 (MQ=255) gAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTaa > 1:330241/1‑69 (MQ=255) atatCGACTTTACCCAGCTTACTGATAGCAAAGTCTGcc > 1:2451420/1‑39 (MQ=255) atatCAGCTTTACCCAGCTTACTGATAGCAAAGTCTGcc > 1:6171/1‑39 (MQ=255) atatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGcc > 1:2114202/1‑39 (MQ=255) atatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGcc > 1:1720440/1‑39 (MQ=255) tatCAACTTTACCCAGCTTACTGATAGCAAAGTCTg > 1:1037429/1‑36 (MQ=255) tatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAatat > 1:2114829/1‑69 (MQ=255) tatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAatat > 1:246552/1‑69 (MQ=255) tatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAatat > 1:2606782/1‑69 (MQ=255) tatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAatat > 1:3038472/1‑69 (MQ=255) tatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAatat > 1:573834/1‑69 (MQ=255) tatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAatat > 1:2064045/1‑69 (MQ=255) tatCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAatat > 1:1109793/1‑69 (MQ=255) aaCTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATcaca > 1:1573228/1‑69 (MQ=255) aaCTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATcac > 1:3252839/1‑68 (MQ=255) aaCTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATcac < 1:884470/68‑1 (MQ=255) cccAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggc < 1:3236066/67‑1 (MQ=255) cccAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggc < 1:937714/67‑1 (MQ=255) cccAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggc < 1:2683295/67‑1 (MQ=255) cAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggcagg > 1:415805/1‑68 (MQ=255) aGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggcaggca < 1:1429441/69‑1 (MQ=255) aGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggcaggca < 1:824011/69‑1 (MQ=255) aGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggcaggca < 1:1559566/69‑1 (MQ=255) aGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggcaggca < 1:2556069/69‑1 (MQ=255) aGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggcaggca < 1:1741811/69‑1 (MQ=255) aGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACggcaggca < 1:2270029/69‑1 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTcc > 1:1175067/2‑38 (MQ=25) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:2453867/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:2895681/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:1474168/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:2662121/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:736583/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:724803/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:3109928/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:656121/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:1589702/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:3367592/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:2111455/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:2115350/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:507080/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:462576/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:2247516/2‑68 (MQ=255) acTTACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCGCAGCggcaggca > 1:3423069/2‑68 (MQ=255) ttACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCgg < 1:2095531/43‑1 (MQ=37) ttACTGACGGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCgg < 1:2671177/43‑1 (MQ=37) gATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAatat > 1:2443449/1‑47 (MQ=255) | TCAAACGGTTTAGGTCCACCGCCACCGGCGTTGTTAACCAGAATATCAACTTTACCCAGCTTACTGATAGCAAAGTCTGCCAGTGCAGAGAGTTCCTGTTCGGAAGTAATATCACAACGGCAGGCA > minE/1025418‑1025543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |