| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,029,310 | T→G | 62.5% | A221A (GCT→GCG) | malY → | bifunctional beta‑cystathionase, PLP‑dependent and regulator of maltose regulon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,029,310 | 0 | T | G | 62.5% | 68.7 / 56.6 | 77 | A221A (GCT→GCG) | malY | bifunctional beta‑cystathionase, PLP‑dependent and regulator of maltose regulon |
| Reads supporting (aligned to +/- strand): ref base T (19/10); new base G (21/27); total (40/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.88e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTG > minE/1029245‑1029374 | tCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCt > 1:2880159/1‑66 (MQ=255)tCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTcg > 1:1153538/1‑68 (MQ=255)tCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTcg > 1:377521/1‑68 (MQ=255) cGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTcgcg < 1:3350597/68‑1 (MQ=255) cATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGga < 1:788278/60‑1 (MQ=255) tatggatatggTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGga < 1:438896/58‑1 (MQ=255) gatatggTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTa > 1:1823211/1‑69 (MQ=255) gCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCaa < 1:2308592/69‑1 (MQ=255) gCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCaa < 1:870298/69‑1 (MQ=255) gCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCaa < 1:332972/69‑1 (MQ=255) gCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGGCGGGCTCGAAAAGTTTCaa < 1:595008/69‑1 (MQ=255) ttCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTc > 1:1796706/1‑69 (MQ=255) tGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGcc < 1:322748/69‑1 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:2230893/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:226395/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:3404353/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:2133167/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:2126928/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:2370614/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:2125556/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:2408726/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:3405953/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:463618/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:495727/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:562885/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:745870/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:843544/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:969830/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:196247/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:1082083/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:1413137/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:160232/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:1860617/1‑54 (MQ=255) tGGAGTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTCGGGATCAAAAAGt > 1:2051095/1‑54 (MQ=255) gTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTg < 1:558033/68‑1 (MQ=255) gTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTg < 1:1598490/68‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTACCGTc < 1:3207790/37‑1 (MQ=38) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:3302178/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1336242/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:2295784/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:50113/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1485225/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1491348/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1269107/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1544940/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:35247/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1589752/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:181419/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:2179984/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1742449/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:3324522/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:2697089/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1810274/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1016440/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1125127/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:2464614/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:2529130/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:798101/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1754112/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:2785373/37‑1 (MQ=255) gTAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:755697/37‑1 (MQ=255) gTAATGGGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:2934902/37‑1 (MQ=38) tAATGTGGCGCGCGGAGACTGGGCGTTGCTAACGTc < 1:1919162/36‑1 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:1194399/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:1183579/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:70096/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:3330679/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:89905/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:1936235/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:541465/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:1542236/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:2923790/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:1800516/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:2627285/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:231430/1‑69 (MQ=255) aTGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCg > 1:2270326/1‑69 (MQ=255) tgGCTCGCGGAGACTGGGCGTTGCTATCGTCGGGCTCGAAAAGTTTCTATATTCCCGCCCTGACCGGTg > 1:2460350/1‑69 (MQ=255) tgGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTg > 1:2675221/1‑69 (MQ=255) | TCCGATGAAATCCATATGGATATGGTTTGGGGCGAGCAGCCGCATATTCCCTGGAGTAATGTGGCTCGCGGAGACTGGGCGTTGCTAACGTCGGGCTCGAAAAGTTTCAATATTCCCGCCCTGACCGGTG > minE/1029245‑1029374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |