| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,036,647 | G→A | 35.7% | V680I (GTT→ATT) | rsxC → | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,036,647 | 0 | G | A | 35.7% | 60.5 / 37.1 | 67 | V680I (GTT→ATT) | rsxC | fused predicted 4Fe‑4S ferredoxin‑type protein |
| Reads supporting (aligned to +/- strand): ref base G (27/16); new base A (7/17); total (34/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
CTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAA > minE/1036582‑1036715 | cTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTg < 1:2441299/69‑1 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:2800490/1‑68 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:1135643/1‑69 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:2550010/1‑69 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:2207789/1‑69 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:343848/1‑69 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:2133436/1‑69 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:343971/1‑69 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:47651/1‑69 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:1571583/1‑69 (MQ=255) gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcg > 1:153770/1‑69 (MQ=255) aGCCAAAGCGCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:1557112/69‑1 (MQ=255) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:1640959/69‑1 (MQ=255) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:853290/69‑1 (MQ=255) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:788752/69‑1 (MQ=255) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:216916/69‑1 (MQ=255) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:2573398/69‑1 (MQ=255) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:2109993/69‑1 (MQ=255) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:1978570/69‑1 (MQ=255) aGCCAAAGCGCGCAAACGGGAACAGCAACCGGATAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaa < 1:1414262/69‑1 (MQ=255) cgcgcAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg > 1:304309/1‑69 (MQ=255) ggAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:2869454/69‑1 (MQ=255) aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2712753/2‑67 (MQ=11) aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2802934/2‑67 (MQ=11) aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2280364/2‑67 (MQ=11) aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2375354/2‑67 (MQ=11) aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2943849/2‑67 (MQ=11) aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:2998915/2‑67 (MQ=11) aggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCcgt > 1:762483/2‑67 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2975563/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:3291422/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:857537/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:427945/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:955797/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2800153/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:1227361/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2421909/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2296654/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2286288/69‑4 (MQ=11) ggCTAATGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:2202809/69‑4 (MQ=11) tGCGGAGCCAGAAGAACAGATTGATCCGCGCAAAGCCGCTGTCGAAGCGGCTATTGCCcgtga < 1:752547/63‑4 (MQ=11) cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCa > 1:2889386/1‑68 (MQ=255) cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCa > 1:748598/1‑68 (MQ=255) cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCa > 1:3334150/1‑68 (MQ=255) cGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGAGGCTATTGCACGCGCCAAAGCa > 1:308637/1‑68 (MQ=255) ggAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:2009271/48‑1 (MQ=255) gAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:2862165/47‑1 (MQ=255) ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:2735994/69‑1 (MQ=255) ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:604434/69‑1 (MQ=255) ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:378281/69‑1 (MQ=255) ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:2541943/69‑1 (MQ=255) ccAGAAGAACAGATTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCGCGCAAg < 1:2927672/69‑1 (MQ=255) aagaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg < 1:3222271/69‑1 (MQ=255) aagaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg < 1:1840138/69‑1 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCTGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1491741/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:2267947/39‑1 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcgcg > 1:3272631/1‑50 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1046894/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1220818/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1364305/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:769768/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:231427/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:693893/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:2311160/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:1980714/1‑68 (MQ=255) agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:380630/1‑68 (MQ=255) agaaCAGGTTGATACGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTgg > 1:3113409/1‑68 (MQ=255) gTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa < 1:3003422/69‑1 (MQ=255) | CTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAA > minE/1036582‑1036715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |