| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,037,015 | A→C | 29.3% | V61V (GTA→GTC) | rsxD → | predicted inner membrane oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,037,015 | 0 | A | C | 29.3% | 57.2 / 36.7 | 58 | V61V (GTA→GTC) | rsxD | predicted inner membrane oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base A (24/17); new base C (11/6); total (35/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
CTCGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCT > minE/1036962‑1037079 | ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:1756757/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:1258474/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:1696699/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:1370038/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:474290/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:1461171/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:386943/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:3400495/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:3398719/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCa > 1:2853124/1‑67 (MQ=255)ctcGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTAGTACTCAAACTACGc > 1:2939594/1‑66 (MQ=255) tCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTc < 1:2317680/69‑1 (MQ=255) tCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCa > 1:2206695/1‑61 (MQ=255) tCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCa > 1:1047117/1‑61 (MQ=255) tCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCa > 1:25905/1‑61 (MQ=255) tCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCa > 1:660476/1‑61 (MQ=255) tCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCa > 1:807694/1‑61 (MQ=255) tCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCa > 1:1249717/1‑61 (MQ=255) tCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAACCTCTCGTACTCAAACTACGCAAGCa > 1:1587423/1‑61 (MQ=255) tCCTGTTGGCATC‑GTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCa > 1:2317609/1‑60 (MQ=255) ggCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAAc > 1:65755/1‑69 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:3010080/69‑1 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:99564/69‑1 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:1350729/69‑1 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:571809/69‑1 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:2772357/69‑1 (MQ=255) ggTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:3377708/68‑1 (MQ=255) ttAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGaaa < 1:659864/68‑1 (MQ=255) ttAGTGCTATGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGaaa < 1:2297133/68‑1 (MQ=255) aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGc < 1:1808305/66‑1 (MQ=255) aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCAtt < 1:2877315/69‑1 (MQ=255) aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCAtt < 1:3004360/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCTGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:221015/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:359722/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:15712/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:728430/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:3027985/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:1709890/69‑1 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCaa > 1:1840134/1‑43 (MQ=38) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:3409183/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:2006612/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:147662/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1469886/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1397988/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:3139442/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1251899/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:813178/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:2331652/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1651220/1‑68 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:1886223/68‑1 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt > 1:3343237/1‑68 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt > 1:2083345/1‑68 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1533595/1‑68 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1600081/1‑68 (MQ=255) gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt < 1:1985353/67‑1 (MQ=255) gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt < 1:2972689/67‑1 (MQ=255) gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt < 1:1547181/67‑1 (MQ=255) gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt < 1:2294686/67‑1 (MQ=255) | CTCGTTCAGATCCTGTTGGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCT > minE/1036962‑1037079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |