| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,037,041 | C→T | 10.7% | A70V (GCC→GTC) ‡ | rsxD → | predicted inner membrane oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,037,041 | 0 | C | T | 10.7% | 199.8 / 7.8 | 101 | A70V (GCC→GTC) ‡ | rsxD | predicted inner membrane oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (22/68); new base T (6/5); total (28/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.06e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
GGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAG > minE/1036979‑1037095 | ggCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAAc > 1:65755/1‑69 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:3010080/69‑1 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:1350729/69‑1 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:571809/69‑1 (MQ=255) cGGTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:2772357/69‑1 (MQ=255) ggTCAGTGCTCTTCTGGCCGAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGa < 1:3377708/68‑1 (MQ=255) ttAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGaaa < 1:659864/68‑1 (MQ=255) ttAGTGCTATGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGaaa < 1:2297133/68‑1 (MQ=255) aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGc < 1:1808305/66‑1 (MQ=255) aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCAtt < 1:2877315/69‑1 (MQ=255) aGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCAtt < 1:3004360/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCTGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:221015/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:15712/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:359722/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:3027985/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:1709890/69‑1 (MQ=255) cGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:728430/69‑1 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCaa > 1:1840134/1‑43 (MQ=38) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1397988/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:3409183/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:147662/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:3139442/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:813178/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:2006612/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:2331652/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1251899/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1651220/1‑68 (MQ=255) gAAGCTCTCGTCCTCAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1469886/1‑68 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt < 1:1886223/68‑1 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1600081/1‑68 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt > 1:2083345/1‑68 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt > 1:3343237/1‑68 (MQ=255) gAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCt > 1:1533595/1‑68 (MQ=255) gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt < 1:1547181/67‑1 (MQ=255) gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt < 1:1985353/67‑1 (MQ=255) gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt < 1:2294686/67‑1 (MQ=255) gTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCt < 1:2972689/67‑1 (MQ=255) cTCAAACTACGCAATCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt < 1:434884/65‑1 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt < 1:3159904/65‑1 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt > 1:2826912/1‑65 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt < 1:1590212/65‑1 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt > 1:2933331/1‑65 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt > 1:2704759/1‑65 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt < 1:2638276/65‑1 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt > 1:2576733/1‑65 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt > 1:2440288/1‑65 (MQ=255) cTCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt > 1:364916/1‑65 (MQ=255) tCAAACTACGCAAGCAGTCGGTAGTCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCtt < 1:622340/64‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAATATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:1602471/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:3017599/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:540832/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:48683/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:563403/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2661576/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:415798/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2878883/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:3163606/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:932738/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:1184421/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2070379/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2726499/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:119464/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:1913132/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:1465039/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2823814/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2500688/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2148084/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2286065/69‑1 (MQ=255) cAAACTACGCAAGCAGTCGGCAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:2141211/69‑1 (MQ=255) tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc < 1:2032675/63‑1 (MQ=255) tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc < 1:3252139/63‑1 (MQ=255) tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc < 1:2246758/63‑1 (MQ=255) tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc < 1:638888/63‑1 (MQ=255) tACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc < 1:1271786/63‑1 (MQ=255) aCGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc < 1:2157635/62‑1 (MQ=255) cGCAAGCAGTCGGGAGCTGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCt < 1:1702929/62‑1 (MQ=255) gCAAGCAGTCGGTAGCTGCAACGTTGAAAGATAACt < 1:402539/36‑1 (MQ=255) cAAGCAGTCGTTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:3047546/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:1668582/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:1676279/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:1734249/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:1554610/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:85543/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:2085565/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:2075246/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:3216739/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:2418930/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:2640606/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:1144082/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:2995014/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:2724518/69‑1 (MQ=255) cAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:2932133/69‑1 (MQ=255) aaGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAg < 1:3220427/68‑1 (MQ=255) aGCAGTCGGTAGCCGTAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa > 1:3189989/1‑66 (MQ=255) aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa > 1:2057668/1‑66 (MQ=255) aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa > 1:457108/1‑66 (MQ=255) aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa > 1:63505/1‑66 (MQ=255) aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTaa > 1:2916252/1‑66 (MQ=255) aGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGAGGTaa > 1:724407/1‑66 (MQ=255) cGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGc < 1:3333831/51‑1 (MQ=255) | GGCATCGGTTAGTGCTCTGTTAGCCGAAGCTCTCGTACTCAAACTACGCAAGCAGTCGGTAGCCGCAACGTTGAAAGATAACTCAGCATTGCTGACAGGCTTATTGCTGGCGGTAAG > minE/1036979‑1037095 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |