| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,037,993 | G→A | 100% | T33T (ACG→ACA) | rsxG → | predicted oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,037,993 | 0 | G | A | 81.0% | 67.2 / 8.2 | 47 | T33T (ACG→ACA) | rsxG | predicted oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (8/1); new base A (25/13); total (33/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACGTTGGCGCTATTTGCAGCGGGTTCAACAGGGTTAACTGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGTGCTGCCAGCCGAACGC > minE/1037925‑1038056 | aCGTTGGCGCTATTTGCAGCGGGTTCAACAGGGTTAACTGCGGCCATCAACCAGATGACCAAAacgacg < 1:666109/69‑1 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:61331/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:3103620/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:3254630/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:3416440/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:2852020/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:2817946/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:42754/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:2623759/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:59200/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:2370970/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:2210758/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:2124537/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:2084606/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:1963829/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:1105424/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:1326670/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:1097706/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:1649369/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:166911/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:1745119/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:1865512/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAg > 1:1870541/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTATGCAACAAAAg > 1:312301/1‑69 (MQ=255) gggTTAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGAACAGGCAAGTCTGCAACAAAAg > 1:2564582/1‑69 (MQ=255) gggATAACTGCGGCCATCAACCAGATGACCAAAACGACAATTGCTGaa > 1:397746/1‑48 (MQ=39) gaaaaCTGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGc < 1:2010565/66‑1 (MQ=255) gTTAACTGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGc > 1:576856/1‑69 (MQ=255) gTTAACTGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGc > 1:507536/1‑69 (MQ=255) gTTAACTGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGc > 1:486568/1‑69 (MQ=255) gTTAACTGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGc > 1:67862/1‑69 (MQ=255) gTTAACTGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGc > 1:1270822/1‑69 (MQ=255) gTTAACTGCGG‑CATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGc > 1:2985400/1‑68 (MQ=255) tGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTAt > 1:751120/1‑68 (MQ=255) tGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTAt > 1:247467/1‑68 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:3094241/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:3132462/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:305352/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:2919736/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:2801806/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:2683846/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:2597091/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:2555934/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:2225102/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:1678502/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:1394220/69‑1 (MQ=255) aaCCAGATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:134234/69‑1 (MQ=255) gATGACCAAAACGACAATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGtg < 1:633675/64‑1 (MQ=255) acgacgATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGTGCTGCCAGCCGAACGc > 1:3289623/1‑69 (MQ=255) acgacgATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGTGCTGCCAGCCGAACGc > 1:2950479/1‑69 (MQ=255) | ACGTTGGCGCTATTTGCAGCGGGTTCAACAGGGTTAACTGCGGCCATCAACCAGATGACCAAAACGACGATTGCTGAACAGGCCAGTCTGCAACAAAAGGCGTTATTTGATCAGGTGCTGCCAGCCGAACGC > minE/1037925‑1038056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |