BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,041,332	G→T	27.7%	L291L (CTG→CTT)	ydgR →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,041,332	0	G	T	27.7%	179.2 / 63.8	119	L291L (CTG→CTT)	ydgR	predicted transporter
Reads supporting (aligned to +/- strand): ref base G (36/50); new base T (22/11); total (58/61)
Fisher's exact test for biased strand distribution p-value = 2.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 120 total aligned reads displayed.

TGCTGCGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTCCATTC  >  minE/1041266‑1041393

tgctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTa                                                             <  1:2920913/69‑1 (MQ=255)

 gctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:587799/69‑1 (MQ=255)

 gctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:2856972/69‑1 (MQ=255)

 gctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:2764774/69‑1 (MQ=255)

 gctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:671389/69‑1 (MQ=255)

 gctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:2343487/69‑1 (MQ=255)

 gctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:466164/69‑1 (MQ=255)

 gctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:822471/69‑1 (MQ=255)

 gctgcGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:1479099/69‑1 (MQ=255)

    gcgcGTCGTAAAATGATCGTTGCCTTCATCCTGATGATCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:2290230/66‑1 (MQ=255)

       cgtcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCa                                                       <  1:2638733/68‑1 (MQ=255)

       cgtcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCa                                                       <  1:2479397/68‑1 (MQ=255)

       cgtcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCa                                                       <  1:1635239/68‑1 (MQ=255)

       cgtcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCa                                                       <  1:1578738/68‑1 (MQ=255)

       cgtcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAg                                                      >  1:511933/1‑69 (MQ=255)

         tcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACATCCAGAt                                                    <  1:4421/69‑1 (MQ=255)

         tcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGAt                                                    <  1:1285661/69‑1 (MQ=255)

         tcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGAt                                                    <  1:3371128/69‑1 (MQ=255)

         tcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGAt                                                    <  1:3284598/69‑1 (MQ=255)

         tcgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGAt                                                    <  1:3187309/69‑1 (MQ=255)

          cgtAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGAt                                                    <  1:1952002/68‑1 (MQ=255)

             aaaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGcc                                                 <  1:1143906/68‑1 (MQ=255)

             aaaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGcc                                                 <  1:770766/68‑1 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGTTGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:3378929/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:2127143/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:1448199/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:2160443/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:506165/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:3258369/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:872712/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:1096182/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:2646858/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:2688934/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:660557/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:1073555/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:2836576/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:2845143/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:62524/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:1385428/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:2086409/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:2003462/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:419532/1‑69 (MQ=255)

              aaaTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTTTATAGCCAGATGCCaa                                               >  1:1793746/1‑69 (MQ=255)

                aTGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTAc                                                            <  1:323408/54‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:3370235/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:1018823/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:1477873/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:1570145/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:427704/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:852100/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:1805260/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:2184456/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGt                                            <  1:851707/69‑1 (MQ=255)

                 tGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTAGTGCTGTACAGCCAGATGCCAACGt                                            <  1:1505629/69‑1 (MQ=255)

                              catcatGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:3318895/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTg                                                               >  1:1233145/1‑37 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCa                                                       >  1:3155741/1‑45 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:383837/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:1761683/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:1976224/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:2724112/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:1556174/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:1551455/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:1027968/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTc                                           >  1:2048293/1‑57 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:1011591/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:765247/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:114196/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:1173192/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:866885/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:1862718/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:2127663/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:2261458/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:2496219/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:252031/1‑69 (MQ=255)

                              cATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActtctt                               >  1:2694789/1‑69 (MQ=255)

                                       gCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAActt                                  >  1:1774037/1‑57 (MQ=255)

                                       gCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTc                       >  1:2203098/1‑68 (MQ=255)

                                       gCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTc                       >  1:2023671/1‑68 (MQ=255)

                                       gCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTc                       >  1:1728032/1‑68 (MQ=255)

                                       gCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTc                       >  1:1268969/1‑68 (MQ=255)

                                               ccATTATCTTCTTCGTGCTTTATAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTg              <  1:2608087/69‑1 (MQ=255)

                                               ccATTATCTTCTTCGTGCTTTATAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTg              <  1:571734/69‑1 (MQ=255)

                                               ccATTATCTTCTTCGTGCTTTATAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTg              <  1:514928/69‑1 (MQ=255)

                                               ccATTATCTTCTTCGTGCTTTATAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTg              <  1:11108/69‑1 (MQ=255)

                                               ccATTATCTTCTTCGTGCTTTATAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTg              <  1:1260405/69‑1 (MQ=255)

                                               ccATTATCTTCTTCGTGCTTTATAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTg              <  1:2158917/69‑1 (MQ=255)

                                               ccATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTg              >  1:909949/1‑69 (MQ=255)

                                               ccATTATCTTCTTCGTGCCTTATAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTg              <  1:1001607/69‑1 (MQ=38)

                                                    aTCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCAGTGAACTTCTTTGCGATTCGTAACGTTGAGCAc         >  1:3113726/1‑69 (MQ=255)

                                                    aTCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCAc         >  1:2631087/1‑69 (MQ=255)

                                                    aTCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCAc         >  1:2622719/1‑69 (MQ=255)

                                                    aTCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCAc         >  1:1238106/1‑69 (MQ=255)

                                                           tcGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTcc      <  1:682531/65‑1 (MQ=255)

                                                           tcGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTcc      <  1:2119402/65‑1 (MQ=255)

                                                           tcGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTcc      <  1:863496/65‑1 (MQ=255)

                                                           tcGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTcc      <  1:900423/65‑1 (MQ=255)

                                                           tcGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTCCAt    <  1:2993755/67‑1 (MQ=255)

                                                           tcGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTCCATTc  >  1:2744447/1‑69 (MQ=255)

                                                           tcGTGCTGTACAGCCAGATGCCAACGTCACTCAACTTCTTTGCGATTCGTAACGTTGAGCACTCCa     <  1:566595/66‑1 (MQ=255)

TGCTGCGCGTCGTAAAATGATCGTTGCCTTCATCCTGATGCTCGAAGCCATTATCTTCTTCGTGCTGTACAGCCAGATGCCAACGTCACTGAACTTCTTTGCGATTCGTAACGTTGAGCACTCCATTC  >  minE/1041266‑1041393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`