| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,042,223 | G→A | 100% | V52V (GTG→GTA) | gst → | glutathionine S‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,042,223 | 0 | G | A | 78.4% | 91.0 / 18.6 | 69 | V52V (GTG→GTA) | gst | glutathionine S‑transferase |
| Reads supporting (aligned to +/- strand): ref base G (3/12); new base A (0/54); total (3/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.68e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACA > minE/1042160‑1042293 | tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:2338845/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:2326767/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:2451472/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:2610013/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:859082/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:3186158/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:1429679/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:1339008/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:525753/68‑1 (MQ=255)tttAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:1386757/68‑1 (MQ=255) aaaCGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACgg < 1:992901/66‑1 (MQ=255) gTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTg < 1:1001344/42‑1 (MQ=255) tACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGaa > 1:3015391/1‑69 (MQ=255) tACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGaa > 1:2821295/1‑69 (MQ=255) tACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGaa > 1:1868905/1‑69 (MQ=255) aaGGGACAGGTACCTGCATTGCTTCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:2473944/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:2890861/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:790690/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:991137/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:2346282/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:988046/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:971577/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:2917405/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:2960691/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:2978980/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:817285/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:3228999/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:380911/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:384991/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:813463/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:391452/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:115267/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:1413902/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:1503419/62‑1 (MQ=255) aaGGGACAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:1517631/62‑1 (MQ=255) acAGGTACCTGCATTGCTGGTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1023603/51‑1 (MQ=255) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1268230/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1368235/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:728098/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:695569/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1317763/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:562262/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1408040/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:517558/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:513714/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:504524/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1283818/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:432579/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:428223/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:2216368/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:2077293/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:2009973/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:922386/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:3242599/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1518327/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:3219301/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:155020/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:3135936/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1082497/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:3038238/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1552937/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1781814/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1078254/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:2927028/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:1947082/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:2006018/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTa < 1:2825336/51‑1 (MQ=37) acAGGTACCTGCATTGCTGCTGGACGATGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:2315891/56‑1 (MQ=38) acAGGTACCTGCATTGCTGCTGGACGATGGTAATTTGCTGACGGAAGGCGTa < 1:305514/51‑1 (MQ=37) gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:569119/51‑1 (MQ=255) gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:498208/51‑1 (MQ=255) gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:3386595/51‑1 (MQ=255) gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:865048/51‑1 (MQ=255) gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:3291959/51‑1 (MQ=255) gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:1810903/51‑1 (MQ=255) gCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGAt < 1:2890228/51‑1 (MQ=255) gCCTGCATTGCTG‑‑GGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACa > 1:2255588/1‑69 (MQ=255) | TTTAATGAAAAAACGTCTCGAAAACGGTGACGATTACTTTGCCGTTAACCCTAAGGGGCAGGTGCCTGCATTGCTGCTGGATGACGGTACTTTGCTGACGGAAGGCGTAGCGATTATGCAGTATCTTGCCGACA > minE/1042160‑1042293 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |