| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 92,124 | G→T | 39.4% | L276L (CTG→CTT) | lpxC → | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 92,124 | 0 | G | T | 39.4% | 46.9 / 54.4 | 66 | L276L (CTG→CTT) | lpxC | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase |
| Reads supporting (aligned to +/- strand): ref base G (20/20); new base T (19/7); total (39/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
TACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGC > minE/92070‑92183 | tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:1019343/1‑69 (MQ=255)tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:875195/1‑69 (MQ=255)tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:819066/1‑69 (MQ=255)tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:378198/1‑69 (MQ=255)tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:1133431/1‑69 (MQ=255)tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:3214288/1‑69 (MQ=255)tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:2302250/1‑69 (MQ=255)tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:2153587/1‑69 (MQ=255)tACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGc > 1:204294/1‑69 (MQ=255) tataAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGaa < 1:992448/69‑1 (MQ=255) tataAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGaa < 1:2977642/69‑1 (MQ=255) tataAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGaa < 1:2457139/69‑1 (MQ=255) tataAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGaa < 1:2450446/69‑1 (MQ=255) tataAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGaa < 1:2448997/69‑1 (MQ=255) tataAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGaa < 1:1140258/69‑1 (MQ=255) tataAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGaa < 1:291614/69‑1 (MQ=255) ataAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGaa < 1:220986/68‑1 (MQ=255) acaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAata < 1:3085873/66‑1 (MQ=255) aaaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAata < 1:504932/68‑1 (MQ=255) aaaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAata < 1:3091871/68‑1 (MQ=255) aaaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAata < 1:2349877/68‑1 (MQ=255) aaaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAata < 1:2989938/68‑1 (MQ=255) aaaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAata < 1:50765/68‑1 (MQ=255) aaaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAata < 1:574192/68‑1 (MQ=255) aaaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAata < 1:1027837/68‑1 (MQ=255) aaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAatat > 1:1168011/1‑68 (MQ=255) aaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAatat > 1:3425152/1‑68 (MQ=255) aaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAatat > 1:2930797/1‑68 (MQ=255) aaTCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAAAAGGAAGACTGGGAatat > 1:84334/1‑68 (MQ=255) ccGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGa < 1:1776961/69‑1 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAATACAGGAAGCCTGCGAATATGTGAc > 1:3249161/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGGATATGTGAc > 1:121838/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:3302302/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:2955809/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:3066856/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:1003648/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:638319/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:743378/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:931287/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:1600639/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:2872488/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:2797483/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:1907686/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:1173207/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:1380715/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:1983851/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:1423754/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:2923839/1‑69 (MQ=255) cGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTTGCAAAACAGGAAGCCTGGGAATATGTGAc > 1:2224614/1‑69 (MQ=255) aCAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAg < 1:399485/69‑1 (MQ=255) aCAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAg < 1:1862603/69‑1 (MQ=255) aCAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAg < 1:1036309/69‑1 (MQ=255) aCAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAg < 1:1112917/69‑1 (MQ=255) aCAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAg < 1:2441562/69‑1 (MQ=255) aCAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAg < 1:2584343/69‑1 (MQ=255) ctgctgCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTg > 1:1580584/1‑69 (MQ=255) ctgctgCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTg > 1:539221/1‑69 (MQ=255) ctgctgCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTg > 1:199871/1‑69 (MQ=255) ctgctgCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTg > 1:68437/1‑69 (MQ=255) ctgctgCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTg > 1:1949240/1‑69 (MQ=255) ctgctgCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTg > 1:1768786/1‑69 (MQ=255) ggCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCGTTg < 1:3255222/67‑1 (MQ=255) ggCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCGTTg < 1:2456466/67‑1 (MQ=255) ggCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCGTTg < 1:189796/67‑1 (MQ=255) ggCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCGTTg < 1:2682133/67‑1 (MQ=255) ggCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGc > 1:1404052/1‑69 (MQ=255) | TACCGCTTATAAATCCGGTCATGCACTGAATAACAAACTGCTGCAGGCTGTCCTGGCGAAACAGGAAGCCTGGGAATATGTGACCTTCCAGGACGACGCAGAACTGCCGTTGGC > minE/92070‑92183 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |