| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,045,174 | C→T | 18.9% | P175P (CCG→CCA) | pdxH ← | pyridoxine 5'‑phosphate oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,045,174 | 0 | C | T | 18.9% | 103.0 / 14.0 | 68 | P175P (CCG→CCA) | pdxH | pyridoxine 5'‑phosphate oxidase |
| Reads supporting (aligned to +/- strand): ref base C (8/47); new base T (6/7); total (14/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAG > minE/1045109‑1045233 | cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:2806229/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:3353595/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:3350036/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:3393244/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:3099511/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:1890395/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:254052/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:1731120/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:482255/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:1572601/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:1561710/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:1555102/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:593830/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:2628096/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:2640957/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:766520/69‑1 (MQ=255)cGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:103585/69‑1 (MQ=255) gATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:2327447/68‑1 (MQ=255) aTGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCaa < 1:796709/69‑1 (MQ=255) cTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:157188/64‑1 (MQ=255) cTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:2958089/64‑1 (MQ=255) cTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:1472981/64‑1 (MQ=255) cTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:2464143/64‑1 (MQ=255) cTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:3412105/64‑1 (MQ=255) ccgcccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGc > 1:1579363/4‑66 (MQ=255) ccgcccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACt > 1:1806786/4‑69 (MQ=255) ccgcccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACt > 1:2110193/4‑69 (MQ=255) ccgcccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACt > 1:1098924/4‑69 (MQ=255) ccgcccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACt > 1:2676949/4‑69 (MQ=255) ccgcccTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACt > 1:1185249/4‑69 (MQ=255) aaCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACTTCGCCCTGTTGa < 1:34178/69‑1 (MQ=255) aaCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACTTCGCCCTGTTGa < 1:3380551/69‑1 (MQ=255) aaCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACTTCGCCCTGTTGa < 1:127887/69‑1 (MQ=255) aaCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACTTCGCCCTGTTGa < 1:1180043/69‑1 (MQ=255) aaCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACTTCGCCCTGTTGa < 1:109705/69‑1 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc > 1:2068530/1‑47 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:1964277/47‑1 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc > 1:322261/1‑47 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc > 1:2408605/1‑47 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:192459/47‑1 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc > 1:1762664/1‑47 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:751152/47‑1 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc > 1:1351261/1‑47 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGaa < 1:851158/69‑1 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGaa < 1:3088854/69‑1 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGaa < 1:2380671/69‑1 (MQ=255) aCTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGaa < 1:1671946/69‑1 (MQ=255) cTCAATCTGTTCATGGCTGACGCGAAAACCGCCCCAAAAGCTCGGc < 1:2234914/46‑1 (MQ=255) cAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACt > 1:1369991/1‑69 (MQ=255) cAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACt > 1:2230780/1‑69 (MQ=255) cAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACt > 1:2058866/1‑69 (MQ=255) gACGCGAAAACCGCCCCAAAAACTTGGCAATGGCACTTCGCCCTGTTGa < 1:564057/49‑1 (MQ=255) aCGCGAAAACCGCCCCAAAAACTTGGCAATGGCACTTCGCCCTGTTGa < 1:2454692/48‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGaa < 1:2728306/69‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGaa < 1:2658547/69‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGaa < 1:3082917/69‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGaa < 1:1327080/69‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGaa < 1:1424527/69‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGaa < 1:3434671/69‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGa < 1:1206628/68‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGa < 1:1849044/68‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGa < 1:2504881/68‑1 (MQ=255) gcgAAAACCGCCCCAAAAGCTCGGCAATGGCACCTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGa < 1:2932699/68‑1 (MQ=255) cgAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGaa < 1:1859674/68‑1 (MQ=255) ccAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAg < 1:2262556/69‑1 (MQ=255) ccAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAg < 1:3188460/69‑1 (MQ=255) ccAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAg < 1:1269939/69‑1 (MQ=255) cAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAg < 1:526554/68‑1 (MQ=255) | CGATGCTCACCACCCTGCCAGAACTCAATCTGTTCAAGGCTGACGCGAAAACCGCCCCAAAAGCTCGGCAATGGCACTTCGCCCTGTTGAAACTTCTGCTTCAGCTCCAGGAATTTACTTTCAAG > minE/1045109‑1045233 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |