| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,047,977 | G→A | 34.6% | P137P (CCG→CCA) | slyB → | outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,047,977 | 0 | G | A | 34.6% | 55.3 / 40.6 | 75 | P137P (CCG→CCA) | slyB | outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base G (5/44); new base A (12/14); total (17/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.20e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
AAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTT > minE/1047918‑1048023 | aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:1167824/68‑1 (MQ=255)aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:2777515/68‑1 (MQ=255)aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:2541163/68‑1 (MQ=255)aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:2460056/68‑1 (MQ=255)aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:2019386/68‑1 (MQ=255)aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:1420448/68‑1 (MQ=255)aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:1301691/68‑1 (MQ=255)aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:1222429/68‑1 (MQ=255) aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:2038847/67‑1 (MQ=255) aCGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCg < 1:2384381/69‑1 (MQ=255) aCGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCg < 1:1307592/69‑1 (MQ=255) aCGAAGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCg < 1:3341873/69‑1 (MQ=255) tACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACg < 1:3366544/54‑1 (MQ=255) aCCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCg > 1:3166553/1‑45 (MQ=255) aCCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc > 1:994596/1‑69 (MQ=255) aCCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc < 1:1588832/69‑1 (MQ=255) aCCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc > 1:976749/1‑69 (MQ=255) atGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc < 1:3218283/63‑1 (MQ=255) atGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc > 1:2860554/1‑63 (MQ=255) atGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc < 1:893469/63‑1 (MQ=255) atGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc < 1:2028020/63‑1 (MQ=255) atGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc < 1:964336/63‑1 (MQ=255) atGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc < 1:3050132/63‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:963189/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:2167769/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:286018/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:784556/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:292105/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:296489/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:2571318/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:3278552/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:571804/69‑1 (MQ=255) ggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:2423919/69‑1 (MQ=255) tACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAg > 1:135164/1‑68 (MQ=255) tACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAg > 1:49654/1‑68 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGc < 1:836115/54‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:588202/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:47708/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:2287873/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:1336556/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:456044/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:2270246/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:818521/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:1646707/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:1670113/69‑1 (MQ=255) cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAAGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:1504396/69‑1 (MQ=255) aCAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGt < 1:2283080/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCATGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:2459044/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:965847/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:3314204/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:740114/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:1376667/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:3176445/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:1836223/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:1919789/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:1983361/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:2414769/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:2348197/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:2105808/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:2187973/69‑1 (MQ=255) aaGGGAACACTCATTTCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGttt < 1:2214172/69‑1 (MQ=255) cacTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:2312861/51‑1 (MQ=255) acTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCa < 1:2773606/50‑1 (MQ=255) ttCTCTCCAGGCCATCGTGTCGTACTGGCCAGCAAt > 1:199913/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:3091009/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:929651/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:2090472/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:228733/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:678966/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:614654/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:2480778/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:1061064/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:3354917/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:2793466/1‑36 (MQ=255) ttCTCTCCAGGCCAACGTGTCGTACTGGCCAGCAAt > 1:2917738/1‑36 (MQ=255) cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTg < 1:1403874/41‑1 (MQ=255) | AAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTT > minE/1047918‑1048023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |