| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,050,528 | G→A | 33.9% | A239A (GCG→GCA) | ydhK → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,050,528 | 0 | G | A | 33.9% | 59.0 / 39.6 | 77 | A239A (GCG→GCA) | ydhK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (24/26); new base A (11/15); total (35/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
TACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACG > minE/1050461‑1050590 | tACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgc > 1:2507796/1‑69 (MQ=255)tACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgc > 1:718423/1‑69 (MQ=255)tACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgc > 1:2591594/1‑69 (MQ=255)tACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgc > 1:2783207/1‑69 (MQ=255) aCTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgcg > 1:2336133/1‑69 (MQ=255) aCTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgcg > 1:1294749/1‑69 (MQ=255) aCTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgcg > 1:3074093/1‑69 (MQ=255) aCTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgcg > 1:3219885/1‑69 (MQ=255) aCTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAcgcgcg > 1:364897/1‑69 (MQ=255) cATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCtt < 1:3319019/67‑1 (MQ=255) cATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCtt < 1:2169224/67‑1 (MQ=255) cATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCtt < 1:3145273/67‑1 (MQ=255) cATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCtt < 1:1427677/67‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:858470/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:459395/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:1314557/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:2509268/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:2160720/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:715377/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:653823/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:1403584/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:633120/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:151821/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:1598343/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:1594498/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:344071/61‑1 (MQ=255) aTTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:337140/61‑1 (MQ=255) tgctgcGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCgctgc > 1:2214236/1‑69 (MQ=255) tgctgcGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCgctgc > 1:2740534/1‑69 (MQ=255) tgctgcGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAAAGCGCGCCTTAATGCgctgc > 1:2070837/1‑69 (MQ=255) cAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCa < 1:3210919/68‑1 (MQ=255) cAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCa < 1:1764449/68‑1 (MQ=255) cAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCa < 1:773931/68‑1 (MQ=255) cAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCa < 1:2580219/68‑1 (MQ=255) aGGCTTTCTTGAGCCACTATCGTTTTCGCCAGCAAAACGCACGCCt < 1:1622718/46‑1 (MQ=39) gCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCTGCAATTa > 1:1382588/1‑69 (MQ=255) gCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTa < 1:2177058/69‑1 (MQ=255) gCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTa < 1:2779139/69‑1 (MQ=255) tttCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACg < 1:3413045/69‑1 (MQ=255) tttCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACg < 1:3343954/69‑1 (MQ=255) tttCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACg < 1:2326393/69‑1 (MQ=255) tttCTGGAGCCACAATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACg < 1:1185543/69‑1 (MQ=255) ttgcTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACg < 1:1040518/66‑1 (MQ=255) ttCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACg < 1:256689/68‑1 (MQ=255) gCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGAcc < 1:651868/68‑1 (MQ=255) gCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGAcc < 1:1577743/68‑1 (MQ=255) cACTATCGTTTTCGCCAGCAAAACGCTCGCCTTAATGCGCTGCTCCACCAGCAATTACg < 1:1773490/59‑1 (MQ=255) cACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAgt < 1:1325565/69‑1 (MQ=255) cACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAgt < 1:1509965/69‑1 (MQ=255) cACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAgt < 1:1532402/69‑1 (MQ=255) aCTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCATCa < 1:1375529/52‑1 (MQ=255) tATCGTTTTCGCCAGCAAAACGGGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTc < 1:2377580/69‑1 (MQ=255) tATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTc < 1:186997/69‑1 (MQ=255) tATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTc < 1:2912015/69‑1 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTTCTCCACCAGCAATTACGTATGACCAGTGTCa > 1:1449707/1‑69 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAAt > 1:1702013/1‑51 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAAt > 1:2516212/1‑51 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAAt > 1:1593796/1‑51 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAAt > 1:1415515/1‑51 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAAt > 1:3124288/1‑51 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAAt > 1:121667/1‑51 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAAt > 1:775424/1‑51 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAAt > 1:2805342/1‑51 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTc > 1:749752/1‑68 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCa > 1:1974826/1‑69 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCa > 1:3006969/1‑69 (MQ=255) aTCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCa > 1:1567699/1‑69 (MQ=255) tCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCAt > 1:700016/1‑69 (MQ=255) tCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCAt > 1:692691/1‑69 (MQ=255) tCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCAt > 1:2347734/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCCCCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:2134789/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGc > 1:60587/1‑68 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:2445742/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:2376164/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:585850/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:539829/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:464296/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:2097200/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:1599598/1‑69 (MQ=255) gCAAAACGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:1728205/1‑69 (MQ=255) gCAAAAAGCACGCCTTAATGCGCTACTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCg > 1:1616/1‑69 (MQ=255) aaaaCGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGa > 1:2162422/1‑68 (MQ=255) aaaaCGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGa > 1:1355838/1‑68 (MQ=255) aaaaCGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGa > 1:2882740/1‑68 (MQ=255) aaaaCGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGa > 1:2807970/1‑68 (MQ=255) aaaCGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACg < 1:1781308/69‑1 (MQ=255) | TACTGACCATGAATTTGCTGCGTATCCAGGCTTTCTGGAGCCACTATCGTTTTCGCCAGCAAAACGCGCGCCTTAATGCGCTGCTCCACCAGCAATTACGTATGACCAGTGTCATCTCCAGCCTGCGACG > minE/1050461‑1050590 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |