| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,050,835 | A→G | 55.8% | T342A (ACC→GCC) | ydhK → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,050,835 | 0 | A | G | 55.8% | 13.8 / 45.4 | 61 | T342A (ACC→GCC) | ydhK | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (8/19); new base G (13/21); total (21/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTA > minE/1050785‑1050889 | tGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACg < 1:738842/64‑1 (MQ=255)tGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACg < 1:2899592/64‑1 (MQ=255)tGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCAACg < 1:1674938/64‑1 (MQ=255)tGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGAGGTGTAGATGATCATACCAGACTCCCACg < 1:847759/64‑1 (MQ=255) aGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTc > 1:833851/1‑69 (MQ=255) aCATCGTGTGCAAAGCGGTGTAGATGATCATACCa > 1:205948/1‑35 (MQ=255) aCATCGTCTGCAAAGCGGTGTAGATGATCATACCa > 1:877537/1‑35 (MQ=255) aCATCGTCTGCAAAGCGGTGTAGATGATCATACCa > 1:670080/1‑35 (MQ=255) aCATCGTCTGCAAAGCGGTGTAGATGATCATACCa > 1:3010975/1‑35 (MQ=255) aCATCGTCTGCAAAGCGGTGTAGATGATCATACCa > 1:3052353/1‑35 (MQ=255) cggcTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:728186/66‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2846438/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:3234478/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:3208410/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2364851/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2382322/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2617935/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2017468/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2972305/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2970133/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2260729/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:3342/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:415282/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:1921998/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:1907782/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:456562/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:172185/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:1602605/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:1058221/69‑1 (MQ=255) cGTCTGCAAAGCGGTGTAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:1015074/69‑1 (MQ=255) gTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:3158317/68‑1 (MQ=255) tCTGCAAAGCGGTGTAGATTATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGAt < 1:2912631/67‑1 (MQ=255) cAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGc > 1:1036825/1‑68 (MQ=255) cAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGc > 1:2460586/1‑68 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:2803202/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:922000/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:1085845/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:1178701/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:780121/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:1411476/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:606229/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:443642/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:4251/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:2016765/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:2277401/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:2616955/1‑69 (MQ=255) gtgtAGATGATCGTGCCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa > 1:3137765/1‑69 (MQ=255) gtgtAGATGATCATACGAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:2929339/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:58126/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:2307262/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:705869/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:153910/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:1891920/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:313543/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:1254275/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:3097276/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:872803/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:3229817/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:894899/69‑1 (MQ=255) gtgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:2254206/69‑1 (MQ=255) tgtAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTa < 1:1790853/68‑1 (MQ=255) | TGCAAAGTAGTCAGGAATTACATCGTCTGCAAAGCGGTGTAGATGATCATACCAGACTCCCACGGACATCCGGCCTGGCTCGTCATACCGATAACGCCGAAGCTA > minE/1050785‑1050889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |