BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,052,841	C→T	12.5%	T161T (ACG→ACA)	ydhF ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,052,841	0	C	T	12.5%	193.4 / 11.1	102	T161T (ACG→ACA)	ydhF	predicted oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (46/43); new base T (9/4); total (55/47)
Fisher's exact test for biased strand distribution p-value = 3.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTC  >  minE/1052776‑1052903

atTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCTGGCGTa                                                             <  1:439069/69‑1 (MQ=255)

atTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCg                                                               >  1:2257555/1‑67 (MQ=255)

atTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCg                                                               >  1:2820344/1‑67 (MQ=255)

atTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCg                                                               >  1:2692287/1‑67 (MQ=255)

atTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCg                                                               >  1:3125056/1‑67 (MQ=255)

atTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTa                                                             <  1:1239146/69‑1 (MQ=255)

 tttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTaa                                                            >  1:2314480/1‑69 (MQ=255)

  ttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTaaa                                                           >  1:3243115/1‑69 (MQ=255)

  ttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTaaa                                                           >  1:2683968/1‑69 (MQ=255)

  ttCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTaaa                                                           >  1:2498355/1‑69 (MQ=255)

   tCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAg                                                          <  1:704905/69‑1 (MQ=255)

   tCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAg                                                          <  1:1916671/69‑1 (MQ=255)

   tCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAg                                                          <  1:2482729/69‑1 (MQ=255)

   tCCACCTGATTAGTGGCAAGAGTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAg                                                          <  1:1381641/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:2911787/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:194686/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1302299/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:2301494/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:2301983/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:3204867/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1338886/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:3202855/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:704843/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:264026/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1959746/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1975040/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:493521/69‑1 (MQ=255)

        cTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1475339/69‑1 (MQ=255)

         tGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1533344/68‑1 (MQ=255)

              aggggCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACg                                                 >  1:727089/4‑67 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACg                                                 >  1:1509399/1‑67 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGc                                                >  1:311767/1‑68 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGc                                                >  1:3016679/1‑68 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGc                                                >  1:35885/1‑68 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGc                                                >  1:3137579/1‑68 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGc                                                >  1:3008682/1‑68 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGc                                                >  1:1548966/1‑68 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGc                                                >  1:1318748/1‑68 (MQ=255)

              aGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGc                                                >  1:1413519/1‑68 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:2905407/68‑1 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:1655004/68‑1 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:1417111/68‑1 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:798268/68‑1 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:2704772/68‑1 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:3296994/68‑1 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:3354839/68‑1 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:358961/68‑1 (MQ=255)

                    aaGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAAt                                          <  1:1973180/68‑1 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAAGAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACCAAAATGa                                        >  1:1770159/3‑68 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACGAAAATGa                                        >  1:1832556/3‑68 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACCAAAGTGa                                        >  1:195276/3‑68 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACCAAAATg                                         >  1:1164629/3‑67 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACCAAAATGa                                        >  1:2850419/3‑68 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACCAAAATGa                                        >  1:1725659/3‑68 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACCAAAATGa                                        >  1:228634/3‑68 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACCAAAATGa                                        >  1:226143/3‑68 (MQ=255)

                      gagTAAATGGCAGGCGTGATTGCAACAGCGCAAATTGCGCAGGTGTAAAGTTTGATACACCAAAATGa                                        >  1:2079972/3‑68 (MQ=255)

                       ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACg                                      >  1:1510701/1‑69 (MQ=255)

                       ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACg                                      >  1:836800/1‑69 (MQ=255)

                       ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACg                                      >  1:527407/1‑69 (MQ=255)

                       ggTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCAAAAATGAc                                       >  1:833407/1‑68 (MQ=255)

                        gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1157494/53‑1 (MQ=255)

                        gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:804291/53‑1 (MQ=255)

                        gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:653254/53‑1 (MQ=255)

                        gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:552441/53‑1 (MQ=255)

                        gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:3363459/53‑1 (MQ=255)

                        gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:2824610/53‑1 (MQ=255)

                        gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1541985/53‑1 (MQ=255)

                        gTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1563327/53‑1 (MQ=255)

                         tAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1361329/52‑1 (MQ=255)

                         tAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCa                                    <  1:1994274/69‑1 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:1079648/50‑1 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCAc                                   >  1:760744/1‑68 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:2031768/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:1603071/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:69901/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:1856311/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:1947906/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:2372476/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:483056/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:264728/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:431285/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:741755/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:1416838/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:2189387/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:3307083/1‑69 (MQ=255)

                           aaCGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACt                                  >  1:3289600/1‑69 (MQ=255)

                                cAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGa                                                     <  1:2196366/45‑1 (MQ=255)

                                           gCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGa                      >  1:3009415/1‑65 (MQ=255)

                                           gCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGa                      >  1:2302806/1‑65 (MQ=255)

                                           gCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGa                      >  1:2019340/1‑65 (MQ=255)

                                           gCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGa                      >  1:2075841/1‑65 (MQ=255)

                                                     aaaTTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGaa        >  1:2610727/1‑69 (MQ=255)

                                                     aaaTTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGaa        >  1:1485872/1‑69 (MQ=255)

                                                     aaaTTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGaa        >  1:3412912/1‑69 (MQ=255)

                                                     aaaTTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGaa        >  1:1250525/1‑69 (MQ=255)

                                                            gcAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCGGCTCTGATGCAGATGTTTGAACGCGTc  <  1:573686/68‑1 (MQ=255)

                                                            gcAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTc  <  1:2066532/68‑1 (MQ=255)

                                                            gcAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTc  <  1:3119449/68‑1 (MQ=255)

                                                            gcAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTc  <  1:2231745/68‑1 (MQ=255)

ATTTCCACCTGATTAGTGGCAAGGGTAAACGGCAGACGTGATTGCAACAGGGCAAATTGCGCAGGCGTAAAGTTCGATACGCCAAAATGACGCACTTTGCCGCTCTGATGCAGATGTTTGAACGCGTC  >  minE/1052776‑1052903

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`