Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,053,053 G→A 22.7% R91C (CGT→TGT)  ydhF ← predicted oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,053,0530GA22.7% 205.7 / 48.2 149R91C (CGT→TGT) ydhFpredicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (84/31);  new base A (5/29);  total (89/60)
Fisher's exact test for biased strand distribution p-value = 2.14e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 149 total aligned reads displayed.

GCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGT  >  minE/1052997‑1053117
                                                        |                                                                
 ctctTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTcgc                                                      >  1:452983/1‑68 (MQ=255)
    ttAATGATGTGATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATa                                                  >  1:1713200/1‑69 (MQ=255)
    ttAATGATGTGATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATa                                                  >  1:760989/1‑69 (MQ=255)
      aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATa                                                  >  1:1595667/1‑67 (MQ=255)
      aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc                                                 >  1:1873947/1‑68 (MQ=255)
      aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc                                                 >  1:717263/1‑68 (MQ=255)
      aaTGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc                                                 >  1:387119/1‑68 (MQ=255)
        tgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc                                                 <  1:2078292/66‑1 (MQ=255)
        tgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc                                                 <  1:2570627/66‑1 (MQ=255)
        tgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc                                                 <  1:2397752/66‑1 (MQ=255)
        tgatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc                                                 <  1:449774/66‑1 (MQ=255)
         gatgTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATAc                                                 <  1:1103229/65‑1 (MQ=255)
          aagtgATCGCGGTCAGTGATGTAATGCCCAATGACGTTTTCTTCACGCGCGGtcgtcg                                                       >  1:1021025/3‑58 (MQ=39)
            gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATAGCGCAtt                                           <  1:435203/68‑1 (MQ=255)
            gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCAtt                                           <  1:774895/68‑1 (MQ=255)
            gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCAtt                                           <  1:931198/68‑1 (MQ=255)
            gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCAtt                                           <  1:195949/68‑1 (MQ=255)
            gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCAtt                                           <  1:309659/68‑1 (MQ=255)
            gtgATCGCGGTCAGTGATGTAATGGCCAATGACGTTTTCTTAACACGCGGTCGTCGCGATACCGCAtt                                           <  1:2378460/68‑1 (MQ=255)
            gtgATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCAtt                                           <  1:2144479/68‑1 (MQ=255)
               aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGa                                                    >  1:2331694/1‑56 (MQ=255)
               aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt                                       >  1:476786/1‑69 (MQ=255)
               aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt                                       >  1:428947/1‑69 (MQ=255)
               aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt                                       >  1:796704/1‑69 (MQ=255)
               aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt                                       >  1:2710832/1‑69 (MQ=255)
               aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt                                       >  1:1754388/1‑69 (MQ=255)
               aTCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACt                                       >  1:3271501/1‑69 (MQ=255)
                 cgcgGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTg                                      >  1:2902793/1‑68 (MQ=255)
                 cgcgGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTg                                      >  1:1741117/1‑68 (MQ=255)
                 cgcgGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTg                                      >  1:3224290/1‑68 (MQ=255)
                 cgcgGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTGACTg                                      >  1:3348294/1‑68 (MQ=255)
                        aGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGa                                                    >  1:2440893/1‑47 (MQ=255)
                        aGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGAt                                                   >  1:3422616/1‑48 (MQ=255)
                          tGATGTAATGGCCTATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGAAGATTTcc                             <  1:636031/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGGCGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:1420764/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:308302/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:3035417/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:3224021/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:2652178/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:2574854/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:2482506/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:2460304/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:1822734/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:2022424/68‑1 (MQ=255)
                          tGATGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:2028035/68‑1 (MQ=255)
                            aTGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATc                          >  1:1716253/1‑69 (MQ=255)
                            aTGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATc                          >  1:341679/1‑69 (MQ=255)
                            aTGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATc                          >  1:511908/1‑69 (MQ=255)
                             tGTAATGGCCAATGACGTTTTCTTCACACGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:1062668/65‑1 (MQ=255)
                               tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCTCGATACCGCATTTACTGACGATTTcc                             <  1:292422/63‑1 (MQ=255)
                               tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:1640393/63‑1 (MQ=255)
                               tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:313364/63‑1 (MQ=255)
                               tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:3016962/63‑1 (MQ=255)
                               tAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:1209053/63‑1 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCa                            >  1:1392881/1‑62 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCa                            >  1:1736128/1‑62 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCa                            >  1:1465863/1‑62 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCa                            >  1:2598755/1‑62 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGtt                      >  1:2717919/1‑68 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGtt                      >  1:249215/1‑68 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGtt                      >  1:2648245/1‑68 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGtt                      >  1:2889223/1‑68 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:3004346/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:384814/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:276905/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:459052/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:3378075/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:2839443/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:2578678/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:219359/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:707604/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:2660021/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTc                     >  1:2943611/1‑69 (MQ=255)
                                 aTGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGAATTCCATCCGTTc                     >  1:538572/1‑69 (MQ=255)
                                     ccAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTcc                             <  1:944115/57‑1 (MQ=255)
                                          gACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtg            >  1:1115647/1‑69 (MQ=255)
                                          gACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtg            >  1:2116472/1‑69 (MQ=255)
                                              ttttCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggc        <  1:937821/69‑2 (MQ=255)
                                              ttttCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggc        <  1:2341983/69‑2 (MQ=255)
                                                ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt        >  1:1539704/1‑67 (MQ=255)
                                                ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt        >  1:2444563/1‑67 (MQ=255)
                                                ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt        >  1:1597757/1‑67 (MQ=255)
                                                ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt        >  1:2589091/1‑67 (MQ=255)
                                                ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt        >  1:2780411/1‑67 (MQ=255)
                                                ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt        >  1:3029492/1‑67 (MQ=255)
                                                ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt        >  1:2005561/1‑67 (MQ=255)
                                                ttcttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCaggtgaggt        >  1:3395553/1‑67 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:1854552/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:3145658/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:609320/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:1464097/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:2794468/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:1361388/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:2287385/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:2283565/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:832236/68‑1 (MQ=255)
                                                  cttcACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGcc     <  1:1020589/68‑1 (MQ=255)
                                                     cACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGt  >  1:985761/1‑68 (MQ=255)
                                                     cACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGt  >  1:1518497/1‑68 (MQ=255)
                                                     cACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGt  >  1:1653618/1‑68 (MQ=255)
                                                        |                                                                
GCTCTTAATGATGTGATCGCGGTCAGTGATGTAATGACCAATGACGTTTTCTTCACGCGCGGTCGTCGCGATACCGCATTTACTGACGATTTCCATCCGTTCACGCAGGTGAGGTGCCAGT  >  minE/1052997‑1053117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑