| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,053,423 | G→A | 44.1% | R63R (CGC→CGT) | ydhL ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,053,423 | 0 | G | A | 44.1% | 30.5 / 89.1 | 114 | R63R (CGC→CGT) | ydhL | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (12/51); new base A (40/10); total (53/61) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.43e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
Only 100 of 129 total aligned reads displayed.
TACGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCATTT > minE/1053357‑1053490 | taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcgc > 1:154202/1‑68 (MQ=255)taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcgc > 1:2917271/1‑68 (MQ=255)taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcgc > 1:980225/1‑68 (MQ=255)taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcgc > 1:3213382/1‑68 (MQ=255)taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcgc > 1:2272633/1‑68 (MQ=255)taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcgc > 1:1093540/1‑68 (MQ=255)taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcgc > 1:1946984/1‑68 (MQ=255)taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcgc > 1:2371047/1‑68 (MQ=255)taCGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTgcg > 1:2747607/1‑67 (MQ=255) gATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTg < 1:2348615/69‑1 (MQ=255) gATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTg < 1:3054830/69‑1 (MQ=255) ttAGGTTCAAAAGAGTGATGGTTGCTCCTGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAAttt > 1:652697/1‑66 (MQ=255) ttAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTgcg > 1:2339097/1‑69 (MQ=255) ttAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTgcg > 1:1926151/1‑69 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCAtt < 1:2243230/68‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCAtt < 1:2851950/68‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:1883397/69‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:2494505/69‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:2659437/69‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:2212034/69‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:983165/69‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:2843479/69‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:3223048/69‑1 (MQ=255) ttCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:468465/69‑1 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACTCGTaa > 1:1694717/1‑54 (MQ=38) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTaa > 1:1524646/1‑54 (MQ=38) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATc > 1:1223560/1‑68 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATc > 1:1032550/1‑68 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:2632797/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:2582871/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:2415020/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:2395309/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:3128166/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:3307834/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:47590/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:631531/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:2451220/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:1629448/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:2173437/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:205894/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:1803228/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:1694820/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:1174487/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:1083129/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCg > 1:1119663/1‑69 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGACTCTGGCTTATTTGCGCGTAATTTGCGCATTAATc < 1:894442/68‑1 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATc < 1:1652598/68‑1 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATc < 1:3288903/68‑1 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATc < 1:936708/68‑1 (MQ=255) aaaGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATc < 1:3361129/68‑1 (MQ=255) aaaGAGTGAT‑GTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGc > 1:3394372/1‑69 (MQ=255) aaGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATc < 1:2434502/67‑1 (MQ=255) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:2188047/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:1442556/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:114311/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:1237320/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:318504/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:3286518/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:2670275/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:668064/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:847871/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:2259900/1‑51 (MQ=38) ggTTGCTCCGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTgcgc > 1:1023237/1‑51 (MQ=38) gTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACaaa > 1:154982/1‑69 (MQ=255) gTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACaaa > 1:2479902/1‑69 (MQ=255) gTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACaaa > 1:2982767/1‑69 (MQ=255) gTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACaaa > 1:3120140/1‑69 (MQ=255) gTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACaaa > 1:2246531/1‑69 (MQ=255) gTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACaa > 1:1308642/1‑68 (MQ=255) tGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAgg < 1:105585/69‑1 (MQ=255) tGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAgg < 1:3026458/69‑1 (MQ=255) tGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAgg < 1:724330/69‑1 (MQ=255) ccGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc < 1:415311/68‑1 (MQ=255) ccGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc < 1:3074220/68‑1 (MQ=255) ccGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc < 1:2452363/68‑1 (MQ=255) ccGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc < 1:2187714/68‑1 (MQ=255) ccGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc < 1:2318761/68‑1 (MQ=255) ccGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc < 1:1013384/68‑1 (MQ=255) ccGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc < 1:1754555/68‑1 (MQ=255) ccGGTTCGTCTGATGGCGCTGGCTTATTTACACGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc < 1:1448926/68‑1 (MQ=255) ccGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGgcgc > 1:2724535/1‑68 (MQ=255) gTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAACGGCgcagc < 1:754322/68‑1 (MQ=255) gTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCgcagc < 1:422176/68‑1 (MQ=255) gTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCgcagc < 1:2100917/68‑1 (MQ=255) gTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCgcagc < 1:1984322/68‑1 (MQ=255) gatgaCGCTGGCTTATTTGCGCGTAATTTGCGCATTa < 1:3339052/37‑1 (MQ=255) gCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCg < 1:1040522/69‑1 (MQ=255) gCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCg < 1:317329/69‑1 (MQ=255) cTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCg < 1:1377060/68‑1 (MQ=255) cTTATTTGCGCGTATTTTGCGCATTAATCGCTGACGACAAAGGCgcagc < 1:2583782/49‑1 (MQ=255) tGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCa < 1:2400779/68‑1 (MQ=255) tGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCa < 1:1206270/68‑1 (MQ=255) tGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCa < 1:1228086/68‑1 (MQ=255) tGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCa < 1:880338/68‑1 (MQ=255) tGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCa < 1:825515/68‑1 (MQ=255) tGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCa < 1:3216741/68‑1 (MQ=255) tGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCa < 1:2979983/68‑1 (MQ=255) tGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCa < 1:3371500/68‑1 (MQ=255) gcgTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCAttt < 1:1756424/68‑1 (MQ=255) gcgTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCAttt < 1:9196/68‑1 (MQ=255) | TACGCAAGATTAGGTTCAAAAGAGTGATGGTTGCTCCGGTTCGTCTGATGACGCTGGCTTATTTGCGCGTAATTTGCGCATTAATCGCTGCCGACAAAGGCGCAGCACCTCTTGTTTTTCGCCATCGCTCATTT > minE/1053357‑1053490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |