| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,053,813 | T→C | 32.7% | L6L (TTA→CTA) ‡ | ydhM → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,053,813 | 0 | T | C | 32.7% | 41.8 / 29.2 | 58 | L6L (TTA→CTA) ‡ | ydhM | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (20/19); new base C (15/4); total (35/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGC > minE/1053747‑1053881 | cATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAAtt > 1:444637/1‑68 (MQ=255)cATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAAtt > 1:2062890/1‑68 (MQ=255)cATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAAtt > 1:1375225/1‑68 (MQ=255) ctcCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACt > 1:1212965/1‑68 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:1008473/68‑1 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:2462003/68‑1 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:3424511/68‑1 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:2370844/68‑1 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:1845240/68‑1 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:1838884/68‑1 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:163864/68‑1 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:1582858/68‑1 (MQ=255) gACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCg < 1:89007/68‑1 (MQ=255) gggCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGa > 1:3402628/1‑69 (MQ=255) ttGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAgg > 1:627333/1‑69 (MQ=255) tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:518159/1‑69 (MQ=255) tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:1103375/1‑69 (MQ=255) tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:219108/1‑69 (MQ=255) tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:888620/1‑69 (MQ=255) tGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAggg > 1:469612/1‑69 (MQ=255) ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:1874319/68‑1 (MQ=255) ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:1199054/68‑1 (MQ=255) ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:1311416/68‑1 (MQ=255) ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:2454001/68‑1 (MQ=255) ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:256294/68‑1 (MQ=255) ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:2717614/68‑1 (MQ=255) ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:2833049/68‑1 (MQ=255) ccTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGt < 1:3320436/68‑1 (MQ=255) cTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAATGGt < 1:3407114/67‑1 (MQ=255) cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:610911/1‑69 (MQ=255) cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:48351/1‑69 (MQ=255) cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:906531/1‑69 (MQ=255) cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:3419975/1‑69 (MQ=255) cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:580387/1‑69 (MQ=255) cAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTc > 1:1771595/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:2900470/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:1011245/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:901675/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:1267001/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:12696/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:1296514/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:534519/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:1955296/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:2345846/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:3431263/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:2619517/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:2835458/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:3238095/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:3042078/1‑69 (MQ=255) cGTGGATTCACCGGGATGGGGCTAAGCGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTAt > 1:3077225/1‑69 (MQ=255) tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:2631105/1‑69 (MQ=255) tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:2946531/1‑69 (MQ=255) tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:3008817/1‑69 (MQ=255) tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:672780/1‑69 (MQ=255) tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:3165146/1‑69 (MQ=255) tCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCactact > 1:3384086/1‑69 (MQ=255) cGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGTTCTAAAGAAGCg < 1:1391079/67‑1 (MQ=255) cGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGTTCTAAAGAAGCg < 1:3170225/67‑1 (MQ=255) cGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGTTCTAAAGAAGCg < 1:319838/67‑1 (MQ=255) cGAACTCCTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGTTCTAAAGAAGCg < 1:2867968/67‑1 (MQ=255) aTTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGGTCTAAAGAAGCGTTTgg < 1:1623495/69‑1 (MQ=255) ttACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGc < 1:2421808/69‑1 (MQ=255) ttACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGc < 1:1194615/69‑1 (MQ=255) ttACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGc < 1:2715279/69‑1 (MQ=255) | CATCTCCTGGCGACGGGCGAGCAACTTTGCCTGCAACGTGGATTCACCGGGATGGGGCTAAGCGAATTACTAAAAACCGCTGAAGTGCCGAAAGGGTCCTTCTATCACTACTTTCGCTCTAAAGAAGCGTTTGGC > minE/1053747‑1053881 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |