BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,063,821	A→G	32.6%	A387A (GCT→GCC)	ydhP ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,063,821	0	A	G	32.6%	75.0 / 50.0	95	A387A (GCT→GCC)	ydhP	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (39/25); new base G (25/6); total (64/31)
Fisher's exact test for biased strand distribution p-value = 6.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 104 total aligned reads displayed.

AATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGAC  >  minE/1063754‑1063889

aaTCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTa                                                                      >  1:415826/1‑68 (MQ=255)

  tCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGc                                                                    <  1:731638/68‑1 (MQ=255)

  tCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGc                                                                    <  1:1059437/68‑1 (MQ=255)

  tCGCACGTTGCCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGc                                                                    <  1:328514/68‑1 (MQ=255)

  tCGCACGTTGCCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGc                                                                    <  1:762656/68‑1 (MQ=255)

  tCGCACGTTGCCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGc                                                                    <  1:3107070/68‑1 (MQ=255)

  tCGCACGTTGCCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGc                                                                    <  1:2785917/68‑1 (MQ=255)

  tCGCACGTTGCCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGc                                                                    <  1:503827/68‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:154820/69‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:3230744/69‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:2787503/69‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:472456/69‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:3156818/69‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:1101730/69‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:2962558/69‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:1946971/69‑1 (MQ=255)

       cGTTGTCAGCAACTGTAACGCAGAAGGTTATCATTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:349105/69‑1 (MQ=255)

        gTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCa                                                              <  1:2882164/68‑1 (MQ=255)

                 aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCa                                                    >  1:897343/1‑69 (MQ=255)

                 aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCa                                                    >  1:3119072/1‑69 (MQ=255)

                 aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCa                                                    >  1:2978872/1‑69 (MQ=255)

                 aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCa                                                    >  1:3286481/1‑69 (MQ=255)

                 aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCa                                                    >  1:2101108/1‑69 (MQ=255)

                 aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCa                                                    >  1:911178/1‑69 (MQ=255)

                 aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCa                                                    >  1:1242669/1‑69 (MQ=255)

                 aaCTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCa                                                    >  1:2452286/1‑69 (MQ=255)

                        aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttgtt                                             <  1:1317604/69‑1 (MQ=255)

                        aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttgtt                                             <  1:1814864/69‑1 (MQ=255)

                        aCGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttgtt                                             <  1:1903455/69‑1 (MQ=255)

                         cGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGgttgtt                                             <  1:1553010/68‑1 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:2958592/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:913930/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:2225272/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:2985569/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:3066804/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:3139939/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:3370604/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:3383294/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:478570/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:576795/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:684649/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:69658/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:735420/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:2094399/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:1940710/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:1870840/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:2037440/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:1861215/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:1429364/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:1624489/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:153706/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:1533775/1‑65 (MQ=255)

                          gCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTGCTTCAGgttg                                               >  1:1096136/1‑65 (MQ=255)

                          gCAGAAGGTTATCATTCTGCGTTTTTGTTTAATTAGCTGTTGGCAACGCAAACTgct                                                       >  1:1475669/1‑55 (MQ=255)

                              aaGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTGGc                                                                    <  1:2886884/40‑1 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:1748618/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:1959355/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:1432292/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:3020807/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:1425658/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:1512793/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:829446/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:2473808/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTgg                                       >  1:105280/1‑68 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTg                                        >  1:1382053/1‑67 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTg                                        >  1:641058/1‑67 (MQ=255)

                               aGGTTATCCTTCTGCGTTTTTGTTAAATAAGCTGTTAGCAACGCaa                                                             >  1:1562422/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:2280194/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:1386283/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:532959/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:580327/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:621699/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:641090/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:354995/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:1132158/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:1520606/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:2220481/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:800318/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:1032865/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:2831675/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:2843127/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:2895050/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:1891766/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTg                                                         >  1:34041/1‑46 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGa                                   <  1:455304/68‑1 (MQ=255)

                                   tATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGa                                   <  1:3285608/68‑1 (MQ=255)

                                               tttttGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAg                       <  1:749390/68‑1 (MQ=255)

                                               tttttGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAg                       <  1:220014/68‑1 (MQ=255)

                                               tttttGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAg                       <  1:2333277/68‑1 (MQ=255)

                                                   tGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCATCaat                   <  1:2029996/68‑1 (MQ=255)

                                                   tGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCaat                   <  1:1784702/68‑1 (MQ=255)

                                                     tttAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGa                                   <  1:2344910/50‑1 (MQ=255)

                                                                   aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACAcc                         <  1:264850/46‑1 (MQ=255)

                                                                   aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACAcc                         <  1:334931/46‑1 (MQ=255)

                                                                   aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACAcc                         <  1:2475622/46‑1 (MQ=255)

                                                                   aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACAcc                         <  1:2155264/46‑1 (MQ=255)

                                                                   aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACAcc                         <  1:2141428/46‑1 (MQ=255)

                                                                   aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGa   >  1:1039307/1‑68 (MQ=255)

                                                                   aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGAc  >  1:1593804/1‑69 (MQ=255)

                                                                   aGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGAAATAAACAcc                         <  1:1364927/46‑1 (MQ=255)

AATCGCACGTTGTCAGCAACTGTAACGCAGAAGGTTATCCTTCTGCGTTTTTGTTTAATTAGCTGTTAGCAACGCAAACTGTTTCAGGTTGTTTTCTGGCTGACATAAACACCAGCAATAATGCCAGTCCCGCGAC  >  minE/1063754‑1063889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`