| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,066,835 | C→G | 42.2% | V219L (GTT→CTT) | ydhB ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,066,835 | 0 | C | G | 42.2% | 36.7 / 84.4 | 83 | V219L (GTT→CTT) | ydhB | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (42/6); new base G (21/14); total (63/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.25e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
ATCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCGCGC > minE/1066787‑1066899 | aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTAt > 1:242289/1‑63 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:1161682/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:978293/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:731875/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:343399/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:3303098/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:2970478/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:2384896/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:2326170/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:23041/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:2133012/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:1933542/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:1895111/1‑69 (MQ=255)aTCCTGCCGAGATACAGGTTACCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCa > 1:433271/1‑69 (MQ=255) ccGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:3021249/69‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:1082630/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:2998814/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:1265069/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:124510/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:152107/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:607244/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:642831/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:691434/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:2069861/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:803423/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:1906275/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:1927246/67‑1 (MQ=255) gagaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAgg < 1:87336/67‑1 (MQ=255) agaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTaa < 1:1979081/69‑1 (MQ=255) agaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTaa < 1:2418623/69‑1 (MQ=255) agaTACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTaa < 1:3069031/69‑1 (MQ=255) cAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACg > 1:3346171/1‑68 (MQ=255) gatgatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAAc < 1:9658/48‑1 (MQ=255) atgatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCg < 1:2198207/69‑1 (MQ=255) atgatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCg < 1:1397697/69‑1 (MQ=255) gatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTa > 1:177743/1‑36 (MQ=255) gatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTa > 1:849466/1‑36 (MQ=255) gatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTa > 1:3408426/1‑36 (MQ=255) gatTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGtt > 1:2350869/1‑69 (MQ=255) gatTACCAGTCAGGCACGACAACTCTTTTTTGGtt > 1:2468504/1‑35 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:1601676/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:954551/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:137553/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:943445/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:419793/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:449595/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:586422/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:808310/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:804311/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2963494/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2905055/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2822710/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:1592164/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:1560874/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:1896302/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2324995/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:14817/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2338369/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcgc > 1:2348122/1‑67 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTcg > 1:2936496/1‑66 (MQ=255) ccAGTCAGGCACGACAAGTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGAGTTcgc > 1:2446048/1‑67 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:161350/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:3279803/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:943532/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:564726/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:1752870/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:3119311/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:2162053/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:738034/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTCAGGTAGCGTTCGCGAGgtg > 1:2587455/1‑69 (MQ=255) tCAGGCACGACAACTCTTTTTTGGTTAGCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGgtg > 1:1759356/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1184706/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1551653/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1553907/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:3131646/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:2222861/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:810312/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:2248189/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1675509/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:464417/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1641772/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:1452828/1‑69 (MQ=255) aCAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCgcgc > 1:2368649/1‑69 (MQ=255) | ATCCTGCCGAGATACAGGTTGCCGATGATTCCCAGTCAGGCACGACAACTCTTTTTTGGTTATCCAGCAACCAGGTAATACGTTTAGGTAGCGTTCGCGAGGTGTCTTCGCGC > minE/1066787‑1066899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |