| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,069,145 | C→T | 29.4% | D14D (GAC→GAT) | cfa → | cyclopropane fatty acyl phospholipid synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,069,145 | 0 | C | T | 29.4% | 41.7 / 16.5 | 44 | D14D (GAC→GAT) | cfa | cyclopropane fatty acyl phospholipid synthase |
| Reads supporting (aligned to +/- strand): ref base C (11/20); new base T (10/3); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01 | |||||||||||
GATCACCGACCAGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCC > minE/1069078‑1069210 | gATCACCGACCAGTGATGTAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGAc < 1:2923377/68‑1 (MQ=255)gATCACCGACCAGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGAc < 1:1395530/68‑1 (MQ=255)gATCACCGACCAGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGAc < 1:2253651/68‑1 (MQ=255)gATCACCGACCAGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGAc < 1:2149819/68‑1 (MQ=255)gATCACCGACCAGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACAGGATGAc < 1:1448595/68‑1 (MQ=255) aGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGt < 1:1240453/69‑1 (MQ=255) aGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGt < 1:1270639/69‑1 (MQ=255) aGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGt < 1:613935/69‑1 (MQ=255) aGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGt < 1:3297121/69‑1 (MQ=255) aGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGt < 1:2981186/69‑1 (MQ=255) aGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGt < 1:2973380/69‑1 (MQ=255) aGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGt < 1:1838412/69‑1 (MQ=255) aaCTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGa < 1:2388809/69‑1 (MQ=255) aaCTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGa < 1:686122/69‑1 (MQ=255) aaCTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGa < 1:2223601/69‑1 (MQ=255) aaCTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGa < 1:2062164/69‑1 (MQ=255) aaCTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGa < 1:126617/69‑1 (MQ=255) aaCTATGAGTTCATCGTGTATAGAAGAAGGCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGa < 1:67455/69‑1 (MQ=255) cTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAAt < 1:727027/69‑1 (MQ=255) tATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAAtt < 1:1906507/69‑1 (MQ=255) tATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAAtt < 1:514516/69‑1 (MQ=255) cGTGTATAGAAGAAGTCAGTGTACCGGATGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTg < 1:967317/69‑1 (MQ=255) cGTGTATAGAAGAAGTCAGTGTACCGGATGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTg < 1:959260/69‑1 (MQ=255) cGTGTATAGAAGAAGTCAGTGTACCGGATGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTg < 1:2037747/69‑1 (MQ=255) gaagaaGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGtat < 1:946038/68‑1 (MQ=255) gaagaaGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGtat < 1:2772560/68‑1 (MQ=255) gaagaaGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGtat < 1:289902/68‑1 (MQ=255) gaagaaGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGtat < 1:3322335/68‑1 (MQ=255) aagaagTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTa > 1:803223/1‑50 (MQ=255) cAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTaa > 1:2393047/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcccc > 1:604867/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcccc > 1:1362848/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcccc > 1:2987812/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcccc > 1:1460742/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcccc > 1:1678814/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcccc > 1:1798671/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcccc > 1:180942/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcccc > 1:2276917/1‑69 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGccc > 1:2143791/1‑68 (MQ=255) tGATAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGcc > 1:1859172/1‑67 (MQ=255) aCAACTGGTACCGTATCGCCAACGAATTACTTAGCCGt > 1:1923999/1‑38 (MQ=255) aCAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCTATTAACgg > 1:2343976/1‑58 (MQ=255) aCAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACgg > 1:3161422/1‑58 (MQ=255) aCAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACgg > 1:436988/1‑58 (MQ=255) aCAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACgg > 1:521890/1‑58 (MQ=255) aCAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACgg > 1:2929725/1‑58 (MQ=255) aCAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACgg > 1:1127688/1‑58 (MQ=255) aCAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACgg > 1:2158893/1‑58 (MQ=255) aCAACTGGTACCGTATCGCCAACCAATTACTTAGc > 1:3221447/1‑35 (MQ=255) | GATCACCGACCAGTGATGGAGAAACTATGAGTTCATCGTGTATAGAAGAAGTCAGTGTACCGGATGACAACTGGTACCGTATCGCCAACGAATTACTTAGCCGTGCCGGTATAGCCATTAACGGTTCTGCCCC > minE/1069078‑1069210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |