| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,071,834 | A→G | 53.8% | K229K (AAA→AAG) | mdtK → | multidrug efflux system transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,071,834 | 0 | A | G | 53.8% | 9.7 / 54.9 | 70 | K229K (AAA→AAG) | mdtK | multidrug efflux system transporter |
| Reads supporting (aligned to +/- strand): ref base A (19/13); new base G (18/19); total (37/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTT > minE/1071770‑1071896 | tGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCAAAACCCg > 1:1312472/1‑69 (MQ=255) ttttCTTACATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCGAt < 1:2634566/68‑1 (MQ=255) gTTTCTTACATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAATCCCGAt < 1:438685/69‑1 (MQ=255) gTTTCTTACATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCGAt < 1:1103953/69‑1 (MQ=255) gTTTCTTACATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCGAt < 1:2494882/69‑1 (MQ=255) gTTTCTTACATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCGAt < 1:900123/69‑1 (MQ=255) gTTTCTTACATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCGAt < 1:169490/69‑1 (MQ=255) tttCTTACATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCGAt < 1:492933/68‑1 (MQ=255) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:1107394/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:1780806/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:2606583/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:1634031/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:1609348/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:3246860/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:371985/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:1331224/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:3372982/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:927878/59‑1 (MQ=38) cATTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:1178269/59‑1 (MQ=38) aTTAAACGCGCCCGCTCGATGCGCGATATTCGTAACGAAAAAGGCACCGCGAAGCCCg < 1:2239959/58‑1 (MQ=38) aCGCGCCCGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGa < 1:563197/68‑1 (MQ=255) cccGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGa > 1:630092/1‑68 (MQ=255) cccGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGa > 1:667816/1‑68 (MQ=255) ctcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:2380259/3‑69 (MQ=255) cgcgATATTCTTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:3115838/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAAGCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:2586653/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:2762116/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:2885572/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:2530081/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:94848/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:2341858/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:1612842/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:1528506/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:1469711/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:1116663/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:1954121/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:1252358/1‑69 (MQ=255) cgcgATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTc > 1:1896273/1‑69 (MQ=255) atatTCGTAACGAAAAAGGCACCGCGAAGCCCGAt < 1:1702107/35‑1 (MQ=37) cGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGcc < 1:2153868/68‑1 (MQ=255) cGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGcc < 1:340227/68‑1 (MQ=255) cGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGcc < 1:2708297/68‑1 (MQ=255) cGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGcc < 1:2014558/68‑1 (MQ=255) cGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGcc < 1:2324192/68‑1 (MQ=255) cGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGcc < 1:2319171/68‑1 (MQ=255) gTAACGAAAAAGGCGCCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:1657461/1‑69 (MQ=255) gTAACGAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGCAACGACTGATTCAACTCGGTTTGCCGa > 1:2916603/1‑69 (MQ=255) gTAACGAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:1269930/1‑69 (MQ=255) gTAACGAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:1300531/1‑69 (MQ=255) gTAACGAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:2065913/1‑69 (MQ=255) gTAACGAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:1589939/1‑69 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTg > 1:1620723/1‑45 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGa > 1:3079211/1‑46 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:2943161/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:147530/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:2838307/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:1410422/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:2784097/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:592909/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:1637987/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:2534560/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:1111982/1‑64 (MQ=255) gAAAAAGGCACCGCGAAGCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGa > 1:934729/1‑64 (MQ=255) cACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACt > 1:84985/1‑69 (MQ=255) cgtaaaaCCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGtt < 1:1223078/66‑1 (MQ=255) cGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGtt < 1:978888/69‑1 (MQ=255) cGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGtt < 1:2235072/69‑1 (MQ=255) cGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGtt < 1:2009384/69‑1 (MQ=255) cGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGtt < 1:887007/69‑1 (MQ=255) cGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGtt < 1:798052/69‑1 (MQ=255) cGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGtt < 1:3003828/69‑1 (MQ=255) | TGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAACGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGATTGCGCTGGCACTGTT > minE/1071770‑1071896 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |