| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,074,351 | T→C | 39.1% | intergenic (+68/‑40) | valW → / → ydhR | tRNA‑Val/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,074,351 | 0 | T | C | 39.1% | 41.8 / 62.2 | 92 | intergenic (+68/‑40) | valW/ydhR | tRNA‑Val/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (30/26); new base C (14/22); total (44/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCATT > minE/1074283‑1074415 | aGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGAtt < 1:885730/69‑1 (MQ=255)aGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGAtt < 1:1780195/69‑1 (MQ=255)aGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGAtt < 1:3328095/69‑1 (MQ=255)aGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGAtt < 1:3110548/69‑1 (MQ=255) ttttCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCTATACGTTAAAAGATTTac < 1:1829752/69‑1 (MQ=255) ttttCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTac < 1:2593520/69‑1 (MQ=255) cTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:2576866/69‑1 (MQ=255) cTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:1962460/69‑1 (MQ=255) cTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:3014092/69‑1 (MQ=255) cTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:3084795/69‑1 (MQ=255) cTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:3137702/69‑1 (MQ=255) cTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:3154931/69‑1 (MQ=255) cTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:3177151/69‑1 (MQ=255) cTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:1742786/69‑1 (MQ=255) tGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:2574746/68‑1 (MQ=255) cttctCCTTTTTCCCTCTGGTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTa < 1:1625415/66‑1 (MQ=255) tctcCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCg < 1:2071892/62‑1 (MQ=255) ctcCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGtt > 1:2506192/1‑69 (MQ=255) ctcCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGtt > 1:477130/1‑69 (MQ=255) ctcCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGtt > 1:577582/1‑69 (MQ=255) ctcCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGtt > 1:2018348/1‑69 (MQ=255) ctcCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGt > 1:2329736/1‑68 (MQ=255) ctcCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACCTTAAAAGATTTACACTGTc > 1:1474127/1‑57 (MQ=255) ctGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGgag > 1:3069455/1‑67 (MQ=255) ctGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGaa > 1:2885141/1‑69 (MQ=255) ctGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGaa > 1:2530917/1‑69 (MQ=255) ttcttcTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAgg > 1:1034227/1‑61 (MQ=255) ttcttcTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAgg > 1:89487/1‑61 (MQ=255) atcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAgg > 1:2112470/2‑61 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGgag < 1:2933058/62‑1 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGc < 1:971014/68‑1 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGc < 1:1019812/68‑1 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGc < 1:2283110/68‑1 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGc < 1:1506126/68‑1 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGc < 1:3026203/68‑1 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGc > 1:2990476/1‑68 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGc < 1:1715047/68‑1 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCt > 1:1988495/1‑69 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCt > 1:109248/1‑69 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCt > 1:399649/1‑69 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCt > 1:2891496/1‑69 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCt > 1:2757869/1‑69 (MQ=255) tcttctCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCt > 1:2300358/1‑69 (MQ=255) tcttctCTGTATACAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGc < 1:1173585/68‑1 (MQ=255) ctGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGc < 1:506065/68‑1 (MQ=255) ctGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGc < 1:862783/68‑1 (MQ=255) ctGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGc < 1:2788923/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:2551055/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:3171057/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:3120636/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:2903414/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:486393/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:2812587/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:2508125/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:1935516/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:2674439/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:1650129/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:1692132/68‑1 (MQ=255) cAATACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:1789583/68‑1 (MQ=255) aaTACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAg > 1:2381739/1‑45 (MQ=255) aaTACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGt > 1:1457635/1‑68 (MQ=255) aaTACGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTg < 1:1282892/67‑1 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGtt > 1:1916749/1‑35 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCt > 1:2590787/1‑53 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTa > 1:1993116/1‑68 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTAc < 1:2188913/69‑1 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTAc > 1:404065/1‑69 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTAc > 1:992689/1‑69 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTAc > 1:1309782/1‑69 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTAc < 1:2833016/69‑1 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTAc > 1:1556656/1‑69 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTAc > 1:2576172/1‑69 (MQ=255) tACGTTAAAAGATTTACACTGTCTTCGTATGCGTGATCAGAAGGAGAATCGCTATGGCAACTTTGTTAc > 1:1569665/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:385569/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:3271196/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:2505514/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:957189/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:1852590/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:2353264/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:521385/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:7699/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:1059194/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:563023/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:129017/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:686135/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:699785/1‑69 (MQ=255) aCGTTAAAAGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACa > 1:712478/1‑69 (MQ=255) aGATCTACTCTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:2718475/69‑1 (MQ=255) aGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:928638/69‑1 (MQ=255) aGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:2551597/69‑1 (MQ=255) aGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:536994/69‑1 (MQ=255) aGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:533294/69‑1 (MQ=255) aGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:116621/69‑1 (MQ=255) aGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:3376339/69‑1 (MQ=255) aGATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:1473530/69‑1 (MQ=255) gATCTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCAtt < 1:269581/68‑1 (MQ=255) | AGATTTTCTTAATCTGGTCTTCTCCTTTTTCCCTCTGTTTCTTCTCTGTATCCAATACGTTAAAAGATTTACACTGTCTTCGTATGCGTTATCAGAAGGAGAATCGCTATGGCAACTTTGTTACAACTTCATT > minE/1074283‑1074415 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |