| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,075,721 | T→C | 49.3% | S300S (AGT→AGC) | ydhS → | conserved hypothetical protein with FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,075,721 | 0 | T | C | 49.3% | ‑6.2 / 67.8 | 73 | S300S (AGT→AGC) | ydhS | conserved hypothetical protein with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base T (22/15); new base C (17/19); total (39/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.52e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01 | |||||||||||
CTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTGGTTTGCCGAG > minE/1075659‑1075781 | cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:2318365/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:372242/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:358034/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:3359661/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:3077338/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:2884842/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:2872448/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:2868474/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:261833/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:2444110/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:677179/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:207371/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:1654661/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:1560485/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:889786/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:1152038/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:755702/69‑1 (MQ=255)cTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:832800/69‑1 (MQ=255) ttGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCa > 1:2489231/1‑67 (MQ=255) ttGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATa > 1:2488466/1‑69 (MQ=255) ttGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATa > 1:3158021/1‑69 (MQ=255) ttGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATa > 1:1795161/1‑69 (MQ=255) gATCGGGTATTTAGATTGATAGTAGAGGAAATCAAATTTGCTGATCCAGACTGGAGCCAACGc < 1:2920050/63‑1 (MQ=255) cGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa < 1:1796322/69‑1 (MQ=255) cGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa < 1:475954/69‑1 (MQ=255) cGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa < 1:2744411/69‑1 (MQ=255) cGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa < 1:1653310/69‑1 (MQ=255) tAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTTGAGTCAACGCATAGCCTTAGAGAGcc > 1:821981/1‑68 (MQ=255) tAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGcc > 1:2008998/1‑68 (MQ=255) tAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGcc > 1:3124981/1‑68 (MQ=255) tAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGcc > 1:701086/1‑68 (MQ=255) tAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGcc > 1:2471498/1‑68 (MQ=255) gATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTg < 1:103532/68‑1 (MQ=255) aTTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTg < 1:168631/67‑1 (MQ=255) gtagAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGAt < 1:689700/69‑1 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:55385/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:1508524/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:2067633/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:3227903/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:2174309/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:311877/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:2286978/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:2919332/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:236016/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:1242093/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:1163065/1‑39 (MQ=255) aaGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTa > 1:103014/1‑39 (MQ=255) gTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGc < 1:173608/69‑1 (MQ=255) tGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTg < 1:605434/69‑1 (MQ=255) tGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTg < 1:1121763/69‑1 (MQ=255) tGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTg < 1:3121055/69‑1 (MQ=255) tGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTg < 1:645418/69‑1 (MQ=255) tGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTg < 1:2105299/69‑1 (MQ=255) tGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTg < 1:1449545/69‑1 (MQ=255) gCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTg < 1:3070106/68‑1 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGt > 1:652914/1‑41 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:580724/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:2595918/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:748159/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:3194488/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:3057404/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:2884305/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:2875074/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:2840123/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:2470109/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:2234016/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:144971/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:1258787/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:1073004/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:1071640/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:1043083/1‑42 (MQ=255) ccAGACTGGAGCCAACGCATAGCCTTAGAAAGCCTGAATGTc > 1:1033365/1‑42 (MQ=255) gACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTGGTTTGCCGAg > 1:1469632/1‑69 (MQ=255) | CTTTTGGATCGGGTATTTAGATTGATAGTAGAGGAAATCAAGTTTGCTGATCCAGACTGGAGTCAACGCATAGCCTTAGAGAGCCTGAATGTCGATTCCTTTGCTCAAGCCTGGTTTGCCGAG > minE/1075659‑1075781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |