| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,076,192 | G→A | 29.0% | G457G (GGG→GGA) | ydhS → | conserved hypothetical protein with FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,076,192 | 0 | G | A | 29.0% | 80.9 / 33.2 | 79 | G457G (GGG→GGA) | ydhS | conserved hypothetical protein with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base G (23/33); new base A (5/18); total (28/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGC > minE/1076129‑1076259 | tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:1224745/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:2598357/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:2224657/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:1641089/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGTGGGCAGCGTCCGCTTAAAGTGAAAGATATTCCTTTCCCTGGACTAc > 1:1561542/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:911511/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:858842/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:2933413/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:2779412/1‑68 (MQ=255)tGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTAc > 1:2737625/1‑68 (MQ=255) tttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:968432/69‑1 (MQ=255) tttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:2736850/69‑1 (MQ=255) tttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1640326/69‑1 (MQ=255) ttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa < 1:1061679/69‑1 (MQ=255) ttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa < 1:2154327/69‑1 (MQ=255) ttttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa < 1:472269/69‑1 (MQ=255) tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1891152/67‑1 (MQ=255) tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1203737/67‑1 (MQ=255) tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa > 1:2784898/1‑68 (MQ=255) tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa > 1:1060416/1‑68 (MQ=255) tttATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCa > 1:1235240/1‑68 (MQ=255) gATGCCGGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:3266705/1‑68 (MQ=255) gATGCCCTCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:787229/1‑68 (MQ=255) gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:2437516/1‑68 (MQ=255) gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:2170766/1‑68 (MQ=255) gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:3350062/1‑68 (MQ=255) gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:33372/1‑68 (MQ=255) gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:1078686/1‑68 (MQ=255) gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACa > 1:3039718/1‑68 (MQ=255) gATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCCAGCAATTACa > 1:1600531/1‑68 (MQ=255) cgcgGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAAc < 1:704549/68‑1 (MQ=255) cgcgGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAAc < 1:3102356/68‑1 (MQ=255) aCGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa < 1:2285654/69‑1 (MQ=255) aCGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa < 1:1813946/69‑1 (MQ=255) aCGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGa < 1:1963861/69‑1 (MQ=255) tCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAAt < 1:30388/69‑1 (MQ=255) ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2098149/69‑1 (MQ=255) ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:296943/69‑1 (MQ=255) ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:1737017/69‑1 (MQ=255) ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2797619/69‑1 (MQ=255) ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:3248732/69‑1 (MQ=255) ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2557028/69‑1 (MQ=255) ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2182327/69‑1 (MQ=255) ccGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:444412/69‑1 (MQ=255) cTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTAAAGAAAACAGGGGATGAAATc < 1:2947486/66‑1 (MQ=255) ttaaagttaaagATATCCCTTTCCCTGGGCTACGCGAGc < 1:2146518/39‑1 (MQ=255) ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:556286/39‑1 (MQ=255) ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:645017/39‑1 (MQ=255) ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:2209602/39‑1 (MQ=255) ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:3396276/39‑1 (MQ=255) ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1777116/39‑1 (MQ=255) ttAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:1256279/39‑1 (MQ=255) tAAAGTGAAAGATATCCCTTTCCCTGGGGTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:3371641/1‑69 (MQ=255) tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGc < 1:3305092/38‑1 (MQ=255) tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:904231/1‑69 (MQ=255) tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:1048341/1‑69 (MQ=255) tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:1434324/1‑69 (MQ=255) tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:2720623/1‑69 (MQ=255) tAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGa > 1:2578371/1‑69 (MQ=255) aGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATc < 1:2102108/61‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:434242/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1006432/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1677124/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1827687/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:891151/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1936989/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:850307/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:1943505/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2120397/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:603438/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2132796/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:410742/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:3435040/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2531267/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2705195/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:27734/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:3232017/69‑1 (MQ=255) tatTCCTTTCCCTGGACTACGCGAACAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGa < 1:2837528/69‑1 (MQ=255) tatCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGggagga < 1:423809/48‑1 (MQ=255) ggCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGc > 1:2349506/1‑69 (MQ=255) ggCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGc > 1:2150121/1‑69 (MQ=255) ggCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGc > 1:2914637/1‑69 (MQ=255) ggCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGc > 1:2538725/1‑69 (MQ=255) ggCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGc > 1:1212244/1‑69 (MQ=255) | TGACGTTTTTATTGATGCCCGCGGACAACGTCCGCTTAAAGTGAAAGATATCCCTTTCCCTGGGCTACGCGAGCAATTACAGAAAACAGGGGATGAAATCCCTGATGTTGGCGAAGATTATACGTTACAGC > minE/1076129‑1076259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |