BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,080,838	A→G	28.5%	I228I (ATT→ATC)	ydhV ←	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,080,838	0	A	G	28.5%	114.8 / 49.2	112	I228I (ATT→ATC)	ydhV	predicted oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (33/47); new base G (21/11); total (54/58)
Fisher's exact test for biased strand distribution p-value = 2.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTT  >  minE/1080774‑1080897

tGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAAt                                                           <  1:504259/67‑1 (MQ=255)

   gATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGt                                                       <  1:597551/68‑1 (MQ=255)

   gATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGt                                                       <  1:3407335/68‑1 (MQ=255)

   gATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGt                                                       <  1:2825095/68‑1 (MQ=255)

      cTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCa                                                    >  1:2940737/1‑68 (MQ=255)

      cTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCa                                                    >  1:3127639/1‑68 (MQ=255)

      cTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCa                                                    >  1:712312/1‑68 (MQ=255)

      cTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCa                                                    >  1:8460/1‑68 (MQ=255)

      cTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCa                                                    >  1:851685/1‑68 (MQ=255)

      cTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCa                                                    >  1:1787948/1‑68 (MQ=255)

      cTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCa                                                    >  1:1273664/1‑68 (MQ=255)

      cTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCa                                                    >  1:1187321/1‑68 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAg                                                   >  1:2188922/1‑68 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAg                                                   >  1:969295/1‑68 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGt                                                  >  1:403045/1‑69 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGt                                                  >  1:2731010/1‑69 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGt                                                  >  1:2416642/1‑69 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGt                                                  >  1:2289246/1‑69 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGt                                                  >  1:1190897/1‑69 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGt                                                  >  1:1486320/1‑69 (MQ=255)

       tGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGt                                                  >  1:953542/1‑69 (MQ=255)

           tACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGtcat                                               <  1:201935/68‑1 (MQ=255)

           tACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGtcat                                               <  1:1597261/68‑1 (MQ=255)

           tACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGtcat                                               <  1:957019/68‑1 (MQ=255)

           tACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGtcat                                               <  1:2442818/68‑1 (MQ=255)

           tACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGtcat                                               <  1:639865/68‑1 (MQ=255)

            aCTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGtcatca                                             >  1:453735/1‑69 (MQ=255)

            aCTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGtcatca                                             >  1:2718335/1‑69 (MQ=255)

                                gTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                          <  1:901302/68‑1 (MQ=255)

                                gTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                          <  1:966141/68‑1 (MQ=255)

                                gTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                          <  1:1317698/68‑1 (MQ=255)

                                gTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                          <  1:1576506/68‑1 (MQ=255)

                                gTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                          <  1:3419601/68‑1 (MQ=255)

                                gTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                          <  1:1618911/68‑1 (MQ=255)

                                gTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                          <  1:393034/68‑1 (MQ=255)

                                gTAATTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCtt                          <  1:1268775/68‑1 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:3011750/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:2704119/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:3273428/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:115318/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:2183943/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:1040946/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:1708872/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:1656323/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:1917360/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:1327364/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:1269344/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:2074236/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCAtc                      >  1:1214114/1‑69 (MQ=255)

                                   cTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATAGAGACGCTTCAtc                      >  1:2927193/1‑69 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:3365160/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:353258/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:1859540/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:1583394/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:1579486/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:1942144/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:51774/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:3203171/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:73499/67‑1 (MQ=255)

                                        cACGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTcc                   <  1:136089/67‑1 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGa                >  1:2288030/1‑68 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:2089537/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:1318642/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:323995/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:3229251/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:169037/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:1583059/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:1497415/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:629752/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:3020704/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:2181645/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:2021767/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:2415173/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:2578745/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:187306/1‑69 (MQ=255)

                                          cGACATGGTTGTTATTCGCACCGATAAGTTCAGTCATCATGTAATCGTTGAGACGCTTCATCTCCTGAc               >  1:3095359/1‑69 (MQ=255)

                                           gACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg              <  1:2932032/69‑1 (MQ=255)

                                           gACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg              <  1:1480029/69‑1 (MQ=255)

                                           gACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg              <  1:2442788/69‑1 (MQ=255)

                                           gACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg              <  1:1532258/69‑1 (MQ=255)

                                           gACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACg              <  1:2872937/69‑1 (MQ=255)

                                                 gttgttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGc        <  1:2249461/69‑1 (MQ=255)

                                                 gttgttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGc        <  1:1962770/69‑1 (MQ=255)

                                                 gttgttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGc        <  1:888484/69‑1 (MQ=255)

                                                 gttgttATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGc        <  1:1880815/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:140468/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:1804409/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:1206007/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:2203315/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:2269663/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:1699350/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:673775/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:3375253/69‑1 (MQ=255)

                                                       aTTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:2971721/69‑1 (MQ=255)

                                                        ttCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:2441607/68‑1 (MQ=255)

                                                        ttCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:395190/68‑1 (MQ=255)

                                                          cGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:1974494/66‑1 (MQ=255)

                                                          cGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:669669/66‑1 (MQ=255)

                                                          cGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:665547/66‑1 (MQ=255)

                                                          cGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGtt  <  1:1711775/66‑1 (MQ=255)

TGGGATCTGAATACTCTGCCCACGATTGTGGCGTACTTGGCACGACATGGTTGTTATTCGCACCAATAAGTTCAGTCATCATGTAATCATTGAGACGCTTCATCTCCTGACGATCGGCAATGTT  >  minE/1080774‑1080897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`