| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,090,837 | A→G | 45.0% | F288F (TTT→TTC) | sufB ← | component of SufBCD complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,090,837 | 0 | A | G | 45.0% | 7.4 / 48.2 | 60 | F288F (TTT→TTC) | sufB | component of SufBCD complex |
| Reads supporting (aligned to +/- strand): ref base A (6/27); new base G (10/17); total (16/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01 | |||||||||||
TTCGCCTTCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGC > minE/1090771‑1090902 | ttCGCCTTCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGaa > 1:2554292/1‑68 (MQ=255)ttCGCCTTCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGa > 1:2558226/1‑67 (MQ=255) ttCGGACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:1503515/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:1180195/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:2398891/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:1956366/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:1782099/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:2496266/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:256180/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:2733074/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:2750429/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:2997663/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:528922/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:661977/69‑1 (MQ=255) ttCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:80149/69‑1 (MQ=255) tCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:812721/68‑1 (MQ=255) tCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:2182396/68‑1 (MQ=255) acaAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCTGTGTTGTTATCGCCAGGGAACCAAtt < 1:1479191/64‑1 (MQ=255) cACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGGAACCAAtt < 1:1888437/58‑1 (MQ=255) cGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGaa < 1:1792398/69‑1 (MQ=255) cGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGaa < 1:1811377/69‑1 (MQ=255) gCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGaa < 1:2687044/68‑1 (MQ=255) aCGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGa > 1:1777241/1‑59 (MQ=255) aCGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCAcc > 1:1638752/1‑69 (MQ=255) aCGAAGGTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCAcc > 1:435175/1‑69 (MQ=255) aaTACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGtttt < 1:995354/68‑1 (MQ=255) aaTACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGtttt < 1:407400/68‑1 (MQ=255) tACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTGTTTa > 1:2737171/1‑68 (MQ=255) aCCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATg < 1:1228184/69‑1 (MQ=255) ccgccgGTGTTGTTATCGCCAGGACACCAGTTTTGTACCGTGGAat < 1:3368327/46‑1 (MQ=255) ccgccgGTGTTGTTATCGCCAGGAAACCAGTTTTg > 1:780659/1‑35 (MQ=255) ccgccgGTGTTGTTATCGCCAGGAAACCAGTTTTg > 1:2858103/1‑35 (MQ=255) ccgccgGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAat < 1:199535/46‑1 (MQ=255) ggTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:159179/69‑1 (MQ=255) ggTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:652572/69‑1 (MQ=255) ggTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:3185818/69‑1 (MQ=255) ggTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:857410/69‑1 (MQ=255) ggTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:2829190/69‑1 (MQ=255) ggTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:1431013/69‑1 (MQ=255) ggTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:106760/69‑1 (MQ=255) gtgtTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:880721/68‑1 (MQ=255) tgttgtTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGAtgatga < 1:1292803/69‑1 (MQ=255) ttgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATg < 1:1317569/59‑1 (MQ=255) ttgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:874287/65‑1 (MQ=255) ttgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:2952533/65‑1 (MQ=255) ttgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:748279/65‑1 (MQ=255) ttgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGTATGATGACt < 1:1184301/69‑1 (MQ=255) ttgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACt < 1:2679018/69‑1 (MQ=255) ttgttATCGCCAGGAAACAAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:2564934/65‑1 (MQ=255) tgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:2907149/64‑1 (MQ=255) tgttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGgatgat < 1:2582539/64‑1 (MQ=255) gttATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTc < 1:1278360/69‑1 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTccacc > 1:662360/1‑64 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCcaccaccg > 1:2529995/1‑66 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCcaccaccg > 1:3240794/1‑66 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACCGc > 1:1722107/1‑69 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACCGc > 1:735165/1‑69 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACCGc > 1:3251393/1‑69 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACCGc > 1:3143555/1‑69 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACCGc > 1:3012062/1‑69 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACCGc > 1:2838428/1‑69 (MQ=255) aGGGAACCAATTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACCGc > 1:1406453/1‑69 (MQ=255) | TTCGCCTTCGCACAAAGCACGCTTGGTGACGAAGTTGAGAATACCGCCGGTGTTGTTATCGCCAGGAAACCAGTTTTGTACCGTGGAATATTTCACCTCGGCGTTTTTATGGATGATGACTTCCACCACTGC > minE/1090771‑1090902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |