| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,094,838 | G→A | 23.7% | F513F (TTC→TTT) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,094,838 | 0 | G | A | 23.7% | 51.2 / 10.3 | 42 | F513F (TTC→TTT) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (13/19); new base A (3/7); total (16/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.82e-01 | |||||||||||
TGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTA > minE/1094774‑1094903 | tGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGaaaa < 1:3314212/69‑1 (MQ=255)tGAGTCGGTTATGCGGATCAAACTCCGCTTTCACTTTACGCAGTTCTGCAAAAAGTTCCTCACCAaaaa < 1:1368018/69‑1 (MQ=255)tGAGTCGGTTATGCGGATCAAACGCCGCTTTCACTTTACGCAGTTCTGCAAAAAGTTCCTCACCAaaaa < 1:1085278/69‑1 (MQ=255)tGAGTCGGTTATGCGGATCAAACGCCGCTTTCACTTTACGCAGTTCTGCAAAAAGTTCCTCACCAaaaa < 1:3089865/69‑1 (MQ=255)tGAGTCGGTTATGCGGATCAAACGCCGCTTTCACTTTACGCAGTTCTGCAAAAAGTTCCTCACCAaaaa < 1:3176261/69‑1 (MQ=255)tGAGTCGGTTATGCGGATCAAACGCCGCTTTCACTTTACGCAGTTCTGCAAAAAGTTCCTCACCAaaaa < 1:1851169/69‑1 (MQ=255)tGAGTCGGTTATGCGGATCAAACGCCGCTTTCACTTTACGCAGTTCTGCAAAAAGTTCCTCACCAaaaa < 1:1269326/69‑1 (MQ=255) gAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGaaaaa > 1:671547/1‑69 (MQ=255) gAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGaaaaa < 1:2811315/69‑1 (MQ=255) tCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGc > 1:2648831/1‑69 (MQ=255) tCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGc > 1:44007/1‑69 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACg > 1:784605/1‑66 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACg > 1:1867660/1‑66 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACg > 1:498681/1‑66 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACg > 1:1196997/1‑66 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACg > 1:1550397/1‑66 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACg > 1:1444000/1‑66 (MQ=255) ggTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCAACGAAAAACg > 1:66864/1‑66 (MQ=255) gCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTg < 1:501485/68‑1 (MQ=255) gCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTg < 1:1007504/68‑1 (MQ=255) gCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTg < 1:2768414/68‑1 (MQ=255) cGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCCGTa < 1:2531402/69‑1 (MQ=255) tCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTc > 1:2097722/1‑68 (MQ=255) aaaCGCCGCTTTCACTTTACGCAGTTCTGCAAAAAGTTCCTCACCAaaaa < 1:902072/50‑1 (MQ=38) ccGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCTGGCTGTATTCAGCGCGaa < 1:2595822/69‑1 (MQ=255) ccGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGaa < 1:1576979/69‑1 (MQ=255) ccGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGaa < 1:2394857/69‑1 (MQ=255) cgcAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTg < 1:2525167/68‑1 (MQ=255) tCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGaa < 1:1656862/37‑1 (MQ=255) tCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCacaaca < 1:2349588/64‑1 (MQ=255) tCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCacaaca < 1:2316137/64‑1 (MQ=255) tCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAacca > 1:3275268/1‑69 (MQ=255) tCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAacca > 1:561210/1‑69 (MQ=255) ccTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAaccac < 1:2634677/69‑1 (MQ=255) ccTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAaccac < 1:1299212/69‑1 (MQ=255) ccTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAaccac < 1:2151843/69‑1 (MQ=255) ccTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAaccac < 1:2753196/69‑1 (MQ=255) cACCGAAAAACGCCGGTCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCg < 1:2992122/68‑1 (MQ=255) cACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCg < 1:1183250/68‑1 (MQ=255) aCCAAAAAACGCCGGGCTGTATTCGGCGCGAAAACCTTTGCCGTGTTCGCCCCACAACAAACCACCGTa > 1:3409888/1‑69 (MQ=255) aCCAAAAAACGCCGGGCTGTATTCGGCGCGAAAACCTTTGCCGTGTTCGCCCCACAACAAACCACCGTa > 1:702546/1‑69 (MQ=255) aCCAAAAAACGCCGGGCTGTATTCGGCGCGAAAACCTTTGCCGTGTTCGCCCCACAACAAACCACCGTa > 1:1217637/1‑69 (MQ=255) | TGAGTCGGTTATGCGGGTCAAATGCCGCTTTCACTTTGCGCAGTTCTGCAAAAAGTTCCTCACCGAAAAACGCCGGGCTGTATTCAGCGCGAAAACCTTTGCCGTGCTCGCCCCACAACAAACCACCGTA > minE/1094774‑1094903 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |