| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,094,979 | C→T | 60.9% | A466A (GCG→GCA) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,094,979 | 0 | C | T | 60.9% | 44.7 / 66.3 | 97 | A466A (GCG→GCA) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (35/3); new base T (50/9); total (85/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 111 total aligned reads displayed.
AGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCGTGG > minE/1094914‑1095042 | aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:2086978/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:2366275/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:3364402/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:2595001/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:2275880/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:2702655/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:749646/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:3369428/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:2834165/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:2107484/1‑68 (MQ=255)aGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcg > 1:2875958/1‑67 (MQ=255)aGCGCCACCACGTCATCAGAGATATGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAgcgc > 1:2091528/1‑68 (MQ=255) cgcCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTg < 1:3306362/68‑1 (MQ=255) cgcCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTg < 1:2060395/68‑1 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:6247/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:2124696/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:1998109/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:2219122/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:3365032/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:2194030/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:234724/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:3370537/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:1562682/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:1558230/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:2618167/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:3266042/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:1296773/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:1292315/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:2922688/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:3160954/1‑69 (MQ=255) gcCACTACATCATCAGAGATTTGCTTCATCAAAATCTCCTGTTGCGGATCGCACATATCCAGTGCTgga > 1:3116372/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTggacggac > 1:1406394/1‑68 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:2524419/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:2546073/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:2587265/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:2127545/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:3219144/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:1375302/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:2031808/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:1968649/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:1487860/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:1903294/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:3428785/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:1640585/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:485114/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:395678/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGgacggacg > 1:1819127/1‑69 (MQ=255) acGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCAAGCGCTGgacggacg > 1:966934/1‑69 (MQ=255) agagATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGac > 1:1052416/1‑69 (MQ=255) agagATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGac > 1:91842/1‑69 (MQ=255) agagATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGac > 1:3355452/1‑69 (MQ=255) agagATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGac > 1:2436274/1‑69 (MQ=255) agagATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGac > 1:1941796/1‑69 (MQ=255) ctTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACa > 1:2195336/1‑69 (MQ=255) ctTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACa > 1:1478529/1‑69 (MQ=255) ctTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACa > 1:3262202/1‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCGAGACACCTGCGTCGACGTGACCGAACAt > 1:1174692/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAg > 1:3090199/2‑43 (MQ=38) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:3169858/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:320204/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:738006/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:927905/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:3232450/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:879951/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:3273712/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:3331633/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:3364079/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:3377432/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:1073769/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:3084708/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:1270840/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:1294182/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:1690290/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:1903098/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2096911/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2272062/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2310053/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2334363/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2365655/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:3152553/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2706978/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2895035/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2958189/2‑69 (MQ=255) ctgttgCGGATCGCACATATCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACAt > 1:2930382/2‑69 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:385467/69‑1 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:1109978/69‑1 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:721716/69‑1 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:1902632/69‑1 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:2625397/69‑1 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:2902625/69‑1 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:2215405/69‑1 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:3249751/69‑1 (MQ=255) atatCCAGTGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCg < 1:2469718/69‑1 (MQ=255) tCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTTACCGAACATACCGTAGCTTAAGCCGTgg > 1:1512121/1‑69 (MQ=255) tCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGGTTAAGCCGTgg > 1:1328116/1‑69 (MQ=255) tCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCGTgg > 1:1785013/1‑69 (MQ=255) tCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCGTgg > 1:2817624/1‑69 (MQ=255) tCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCGTgg > 1:3046390/1‑69 (MQ=255) tCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCGTgg > 1:1181691/1‑69 (MQ=255) tCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCGTgg > 1:2572937/1‑69 (MQ=255) | AGCGCCACCACGTCATCAGAGATTTGCTTCATCAAAATCTCTTGTTGAGGATCGCACATATCCAGCGCTGGACGGACGTGCAAGACACCTGCGTCGACGTGACCGAACATACCGTAGCTTAAGCCGTGG > minE/1094914‑1095042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |