| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 93,174 | A→G | 38.7% | A52A (GCA→GCG) | secA → | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 93,174 | 0 | A | G | 38.7% | 10.2 / 17.3 | 31 | A52A (GCA→GCG) | secA | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Reads supporting (aligned to +/- strand): ref base A (11/8); new base G (5/7); total (16/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.31e-01 | |||||||||||
GAACCGGAGATGGAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAGGCAA > minE/93112‑93241 | gAACCGGAGATGGAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCt > 1:3297490/1‑68 (MQ=255)gAACCGGAGATGGAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCt > 1:517903/1‑68 (MQ=255)gAACCGGAGATGGAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCt > 1:2084275/1‑68 (MQ=255) ggAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGa < 1:3300890/69‑1 (MQ=255) ggAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGa < 1:1714502/69‑1 (MQ=255) ggAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGa < 1:1963669/69‑1 (MQ=255) ggAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGa < 1:2595457/69‑1 (MQ=255) ggAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGa < 1:3427086/69‑1 (MQ=255) gAAAAACTCTCCGACGAAGAACTGAAATGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGa < 1:3040216/68‑1 (MQ=255) gAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGaa > 1:822229/1‑69 (MQ=255) gAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGaa > 1:2152640/1‑69 (MQ=255) gAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGa < 1:2315153/68‑1 (MQ=255) gAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGa < 1:3305696/68‑1 (MQ=255) ctctCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGAAGTGCt > 1:1295887/1‑68 (MQ=255) gaaCTGAAAGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATc > 1:517654/1‑69 (MQ=255) gaaCTGAAAGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATc > 1:75167/1‑69 (MQ=255) gaaCTGAAAGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATc > 1:2652373/1‑69 (MQ=255) gaaCTGAAAGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATc > 1:153115/1‑69 (MQ=255) gaaCTGAAAGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCCGATc > 1:813588/1‑69 (MQ=255) cTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATccc > 1:2068564/1‑68 (MQ=255) cTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATccc > 1:2203816/1‑68 (MQ=255) aaaGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGAt < 1:515231/62‑1 (MQ=255) aaaGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGAt < 1:3319245/62‑1 (MQ=255) aaaGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGAt < 1:2830105/62‑1 (MQ=255) aaaGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGAt < 1:3229320/62‑1 (MQ=255) aaaGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGAt < 1:3019485/62‑1 (MQ=255) aaaGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGAt < 1:2023724/62‑1 (MQ=255) aaaGGGAAAACCGCAGAGTTTCGTGCGCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGAt < 1:1694466/62‑1 (MQ=255) aCCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGcc > 1:3311630/1‑69 (MQ=255) cACGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAGGCa > 1:3368281/1‑68 (MQ=255) cACGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAGGCa > 1:2047897/1‑68 (MQ=255) aCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAg > 1:604150/1‑44 (MQ=255) aCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAgg > 1:2288744/1‑65 (MQ=255) aCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAGGCaa > 1:2375181/1‑68 (MQ=255) aCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAGGCaa > 1:388374/1‑68 (MQ=255) aCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAGGCaa > 1:1990508/1‑68 (MQ=255) aCGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAGGCaa > 1:1427289/1‑68 (MQ=255) | GAACCGGAGATGGAAAAACTCTCCGACGAAGAACTGAAAGGGAAAACCGCAGAGTTTCGTGCACGTCTGGAAAAAGGCGAAGTGCTGGAAAATCTGATCCCGGAAGCTTTCGCCGTGGTACGTGAGGCAA > minE/93112‑93241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |