BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,099,401	A→T	51.9%	T214S (ACC→TCC)	ydiM →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,099,401	0	A	T	51.9%	16.9 / 195.3	160	T214S (ACC→TCC)	ydiM	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (55/22); new base T (35/48); total (90/70)
Fisher's exact test for biased strand distribution p-value = 2.36e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

TACCTGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAC  >  minE/1099333‑1099467

    tGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCt                                                                 <  1:2343438/68‑1 (MQ=255)

    tGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCt                                                                 <  1:3352068/68‑1 (MQ=255)

    tGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCt                                                                 <  1:388178/68‑1 (MQ=255)

     gTCATAAAGAAAACGACCAGCTCTACGGAACATCGCTGTTGAATTATCGATTTAGCCAGTTATACCTta                                                               <  1:2094915/69‑1 (MQ=255)

     gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               <  1:3405647/69‑1 (MQ=255)

     gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               <  1:1762484/69‑1 (MQ=255)

     gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               <  1:2024809/69‑1 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCtata                                                             >  1:2424602/1‑68 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:588076/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:1009630/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:744512/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:2106947/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:2154980/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:3074500/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:2879768/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:218561/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                            >  1:1192487/1‑69 (MQ=255)

        aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCACTTATTCCCTatat                                                            >  1:211602/1‑69 (MQ=255)

           aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               <  1:3295222/63‑1 (MQ=255)

           aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                         >  1:121663/1‑69 (MQ=255)

           aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                         >  1:1644387/1‑69 (MQ=255)

           aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                         >  1:3054277/1‑69 (MQ=255)

           aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                         >  1:77839/1‑69 (MQ=255)

           aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                         >  1:2085212/1‑69 (MQ=255)

           aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                         >  1:2741665/1‑69 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:2189342/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:2365960/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:2242992/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:1618126/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:2491819/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:1622064/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:688957/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:1088061/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:1141189/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:2947739/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:291788/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:2754389/65‑1 (MQ=255)

                    accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                    <  1:1326218/65‑1 (MQ=255)

                         ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATgg                                            >  1:1679000/1‑68 (MQ=255)

                         ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATgg                                            >  1:2010452/1‑68 (MQ=255)

                         ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                           >  1:3131302/1‑69 (MQ=255)

                         ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                           >  1:3260967/1‑69 (MQ=255)

                         ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                           >  1:2647492/1‑69 (MQ=255)

                         ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                           >  1:2642660/1‑69 (MQ=255)

                         ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                           >  1:1456798/1‑69 (MQ=255)

                           ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                 >  1:2619344/1‑61 (MQ=255)

                           ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                 >  1:3232751/1‑61 (MQ=255)

                           ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                 >  1:84743/1‑61 (MQ=255)

                           ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                 <  1:1159800/61‑1 (MQ=255)

                           ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                 >  1:1419967/1‑61 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               >  1:911588/1‑44 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               >  1:2327641/1‑44 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               >  1:2545035/1‑44 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               >  1:1664417/1‑44 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                               >  1:569059/1‑44 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                      >  1:3089265/1‑69 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                      >  1:1041581/1‑69 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                      >  1:1449828/1‑69 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                      >  1:1861153/1‑69 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                      >  1:1392544/1‑69 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                      >  1:3388585/1‑69 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                      >  1:3161236/1‑69 (MQ=255)

                              cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATAACTta                                                               >  1:3310708/1‑44 (MQ=255)

                                gAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGttt                                    >  1:13760/1‑69 (MQ=255)

                                gAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGttt                                    >  1:1569230/1‑69 (MQ=255)

                                gAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGttt                                    >  1:708393/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCGCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:5705/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCATCGtttt                                   <  1:1304218/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:3244185/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:241008/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:759675/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:840103/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:792249/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:3409721/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:2796819/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:2677679/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:1228619/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:2312266/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:1605402/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:1702274/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:1795235/57‑1 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                       >  1:637987/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                       >  1:1117338/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                       >  1:1253224/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                       >  1:1461924/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                       >  1:3146379/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                       >  1:1708242/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                       >  1:2034692/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                       >  1:2629453/1‑69 (MQ=255)

                                             cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGc                        >  1:698785/1‑68 (MQ=255)

                                              aaTTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                   <  1:1411146/56‑1 (MQ=255)

                                                                 tataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACgg    >  1:2596236/1‑68 (MQ=255)

                                                                  ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc  >  1:1748658/1‑69 (MQ=255)

                                                                  ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc  >  1:391699/1‑69 (MQ=255)

                                                                  ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc  >  1:2226853/1‑69 (MQ=255)

                                                                  ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc  >  1:1317883/1‑69 (MQ=255)

                                                                  ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc  >  1:2540763/1‑69 (MQ=255)

                                                                  ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc  >  1:822864/1‑69 (MQ=255)

                                                                  ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc  >  1:884756/1‑69 (MQ=255)

                                                                  ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc  >  1:1018454/1‑69 (MQ=255)

TACCTGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAC  >  minE/1099333‑1099467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`