BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,099,404	T→C	48.3%	L215L (TTA→CTA)	ydiM →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,099,404	0	T	C	48.3%	4.6 / 160.2	172	L215L (TTA→CTA)	ydiM	predicted transporter
Reads supporting (aligned to +/- strand): ref base T (67/22); new base C (35/48); total (102/70)
Fisher's exact test for biased strand distribution p-value = 1.22e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 176 total aligned reads displayed.

CTGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGACAA  >  minE/1099336‑1099469

 tGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCt                                                                   <  1:3352068/68‑1 (MQ=255)

 tGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCt                                                                   <  1:388178/68‑1 (MQ=255)

  gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 <  1:1660836/69‑1 (MQ=255)

  gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 <  1:1762484/69‑1 (MQ=255)

  gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 <  1:1455225/69‑1 (MQ=255)

  gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 <  1:1428108/69‑1 (MQ=255)

  gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 <  1:2241643/69‑1 (MQ=255)

  gTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 <  1:2024809/69‑1 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:647840/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:1561722/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:1009630/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:141345/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:588076/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:2879768/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:744512/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:746942/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:3074500/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:1192487/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:2440440/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:2106947/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTatat                                                              >  1:2154980/1‑69 (MQ=255)

     aTAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCACTTATTCCCTatat                                                              >  1:211602/1‑69 (MQ=255)

        aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                           >  1:2741665/1‑69 (MQ=255)

        aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                           >  1:2085212/1‑69 (MQ=255)

        aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                           >  1:3054277/1‑69 (MQ=255)

        aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                           >  1:1644387/1‑69 (MQ=255)

        aaGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGc                                                           >  1:77839/1‑69 (MQ=255)

           aaaaCCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCtat                                                        <  1:1380382/69‑1 (MQ=255)

                caccaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   <  1:2197006/69‑1 (MQ=255)

                 accaGCTCTACGGAATATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:2330309/65‑1 (MQ=255)

                 accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:1622064/65‑1 (MQ=255)

                 accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:1088061/65‑1 (MQ=255)

                 accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:1141189/65‑1 (MQ=255)

                 accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:1323013/65‑1 (MQ=255)

                 accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:2242992/65‑1 (MQ=255)

                 accaGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:291788/65‑1 (MQ=255)

                 accaGCTCTACGGAACATCGCTGTGCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:1598406/65‑1 (MQ=255)

                 accaGCTCTACGGAACACCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTatat                                                      <  1:2345038/65‑1 (MQ=255)

                      ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATgg                                              >  1:1679000/1‑68 (MQ=255)

                      ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                             >  1:2642660/1‑69 (MQ=255)

                      ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                             >  1:2647492/1‑69 (MQ=255)

                      ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                             >  1:3260967/1‑69 (MQ=255)

                      ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                             >  1:2980735/1‑69 (MQ=255)

                      ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                             >  1:3131302/1‑69 (MQ=255)

                      ctctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGc                                             >  1:3150125/1‑69 (MQ=255)

                        ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   <  1:2182179/61‑1 (MQ=255)

                        ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   >  1:84743/1‑61 (MQ=255)

                        ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   >  1:1120743/1‑61 (MQ=255)

                        ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   <  1:1295131/61‑1 (MQ=255)

                        ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   <  1:1159800/61‑1 (MQ=255)

                        ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   <  1:253995/61‑1 (MQ=255)

                        ctACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   >  1:2757329/1‑61 (MQ=255)

                         tACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATAtctc                                                   <  1:2608237/60‑1 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 >  1:911588/1‑44 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 >  1:262125/1‑44 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 >  1:2545035/1‑44 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTta                                                                 >  1:3404886/1‑44 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                        >  1:3388585/1‑69 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                        >  1:3264398/1‑69 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                        >  1:2391516/1‑69 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                        >  1:3089265/1‑69 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                        >  1:1223835/1‑69 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                        >  1:1041581/1‑69 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                        >  1:2091200/1‑69 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGt                                        >  1:1861153/1‑69 (MQ=255)

                           cGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATAACTta                                                                 >  1:3310708/1‑44 (MQ=255)

                             gAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGttt                                      >  1:708393/1‑69 (MQ=255)

                             gAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGttt                                      >  1:1948862/1‑69 (MQ=255)

                                       gTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTa                            <  1:3138372/69‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCGCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:5705/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:825359/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:494834/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:1368586/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:3409721/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:1605771/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:1702274/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:1228619/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:792249/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:3244185/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:1795235/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:2312266/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:241008/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:2796819/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:2677679/57‑1 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                         >  1:1253224/1‑69 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                         >  1:637987/1‑69 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                         >  1:1461924/1‑69 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                         >  1:1708242/1‑69 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGcc                         >  1:2034692/1‑69 (MQ=255)

                                          cAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGc                          >  1:698785/1‑68 (MQ=255)

                                           aaTTATCGATTTAGCCAGTTATTCCCTATATGGCTATATCTCAATGGCAACGtttt                                     <  1:1411146/56‑1 (MQ=255)

                                                               ataCCTTATATGGCTATATCTCAATGGCTACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACgg      >  1:2572976/1‑67 (MQ=255)

                                                               ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGa     >  1:3176552/1‑68 (MQ=255)

                                                               ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc    >  1:1317883/1‑69 (MQ=255)

                                                               ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc    >  1:391699/1‑69 (MQ=255)

                                                               ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc    >  1:1712111/1‑69 (MQ=255)

                                                               ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc    >  1:822864/1‑69 (MQ=255)

                                                               ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc    >  1:884756/1‑69 (MQ=255)

                                                               ataCCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGAc    >  1:1018454/1‑69 (MQ=255)

                                                                  ccTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGACaa  <  1:2188993/68‑1 (MQ=255)

CTGTCATAAAGAAAACCACCAGCTCTACGGAACATCGCTGTTCAATTATCGATTTAGCCAGTTATACCTTATATGGCTATATCTCAATGGCAACGTTTTATCTGGTTAGCCAGTGGCTGGCACAGTACGGACAA  >  minE/1099336‑1099469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`