| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 93,594 | G→A | 39.9% | A192A (GCG→GCA) | secA → | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 93,594 | 0 | G | A | 39.9% | 65.2 / 89.8 | 130 | A192A (GCG→GCA) | secA | preprotein translocase subunit, ATPase that targets protein precursors to the SecYE core translocon |
| Reads supporting (aligned to +/- strand): ref base G (33/45); new base A (17/35); total (50/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
AGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTGGACTCCATCCT > minE/93531‑93662 | aGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAg < 1:1859370/69‑1 (MQ=255)aGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAg < 1:214950/69‑1 (MQ=255)aGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAg < 1:2172728/69‑1 (MQ=255)aGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAg < 1:2203809/69‑1 (MQ=255)aGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAg < 1:2775026/69‑1 (MQ=255)aGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAg < 1:2712713/69‑1 (MQ=255)aGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAg < 1:1569820/69‑1 (MQ=255)aGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAg < 1:2208213/69‑1 (MQ=255) aTCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCTCGACAACATGGCGTTCAGCCCTg < 1:3417384/67‑1 (MQ=255) aCTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTgaagaa > 1:513830/1‑69 (MQ=255) aCTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTgaagaa > 1:721967/1‑69 (MQ=255) aCTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTgaagaa > 1:2198900/1‑69 (MQ=255) aCTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTgaagaa > 1:1438957/1‑69 (MQ=255) aCTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTgaagaa > 1:591538/1‑69 (MQ=255) aCTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTgaagaa > 1:2600116/1‑69 (MQ=255) aCTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTgaagaa > 1:2958027/1‑69 (MQ=255) ttACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACg > 1:534901/1‑69 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACgtg < 1:1429139/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACgtg < 1:598004/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACgtg < 1:1404814/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:3013392/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:2175322/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:3157239/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:3171165/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:2043344/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:3350462/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:971524/67‑1 (MQ=255) ggTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:1291285/67‑1 (MQ=255) gTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACgtg < 1:1893465/66‑1 (MQ=255) gTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACgtg < 1:1162826/66‑1 (MQ=255) gTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACgtg < 1:1043112/66‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa < 1:255712/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTTCAACGTa < 1:1605897/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:1826658/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:49065/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:3225193/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:1239876/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:2935515/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:2825793/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:2640282/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:567088/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:683776/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:832255/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:1482419/69‑1 (MQ=255) acaacGAATACGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:880210/69‑1 (MQ=255) aCGGCTTTGACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTa < 1:526566/60‑1 (MQ=255) ggCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTg < 1:120100/40‑1 (MQ=255) tGACTAGCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCt > 1:439655/1‑69 (MQ=255) tGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATgcgc > 1:2148118/1‑68 (MQ=255) tGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCt > 1:3310844/1‑69 (MQ=255) tGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCt > 1:1200442/1‑69 (MQ=255) tGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCt > 1:3418405/1‑69 (MQ=255) tGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCt > 1:296164/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACATCGTAAACt > 1:3200227/1‑56 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:112571/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:947887/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:3098195/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:1476632/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:2145714/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:2865794/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:280082/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:2295761/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:1309555/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:2699484/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:2394200/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:1624388/1‑69 (MQ=255) gACTACCTGCGCGACAACATGGCATTCAGCCCTGAAGAACGTGTACAACGTAAACTGCACTATGCGCtg > 1:2437881/1‑69 (MQ=255) aCCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa > 1:2547755/1‑48 (MQ=255) aCCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa > 1:2240424/1‑48 (MQ=255) aCCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa > 1:1432293/1‑48 (MQ=255) aCCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa > 1:922983/1‑48 (MQ=255) aCCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa > 1:2394425/1‑48 (MQ=255) aCCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa > 1:2025417/1‑48 (MQ=255) aCCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa > 1:3100502/1‑48 (MQ=255) aCCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTa > 1:3093506/1‑48 (MQ=255) ccTGCGCGACAACATGGCGTTCAGCCCTGAAGAACgtg < 1:1408823/38‑1 (MQ=255) gcgcGAGAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:1809960/69‑1 (MQ=255) gcgcGACATCATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:2168117/69‑1 (MQ=255) gcgcGACAAGATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:2925674/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:740159/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:2460020/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:422515/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:1098980/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:1776150/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:1996787/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:2359720/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:2368702/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:2368703/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:3137878/69‑1 (MQ=255) gcgcGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGa < 1:433236/69‑1 (MQ=255) gACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTg < 1:1915813/69‑1 (MQ=255) gACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTg < 1:757058/69‑1 (MQ=255) gACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTg < 1:1614071/69‑1 (MQ=255) gACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTg < 1:1080815/69‑1 (MQ=255) gCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTGGACTCCATc > 1:2245357/1‑69 (MQ=255) gCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTGGACTCCATc > 1:1811316/1‑69 (MQ=255) gCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTGGACTCCATc > 1:420196/1‑69 (MQ=255) gCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTGGACTCCATc > 1:1411652/1‑69 (MQ=255) gTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTGGACTCCATCCt > 1:368903/1‑69 (MQ=255) gTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTGGACTCCATCCt > 1:962602/1‑69 (MQ=255) | AGCTGACATCACTTACGGTACGAACAACGAATACGGCTTTGACTACCTGCGCGACAACATGGCGTTCAGCCCTGAAGAACGTGTACAGCGTAAACTGCACTATGCGCTGGTGGACGAAGTGGACTCCATCCT > minE/93531‑93662 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |