| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,112,816 | C→T | 81.3% | R663H (CGT→CAT) | pps ← | phosphoenolpyruvate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,112,816 | 0 | C | T | 81.3% | 76.2 / 9.9 | 53 | R663H (CGT→CAT) | pps | phosphoenolpyruvate synthase |
| Reads supporting (aligned to +/- strand): ref base C (6/4); new base T (17/26); total (23/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
CACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGG > minE/1112768‑1112869 | cacaTCATGATGATTTTCAGCCCGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGtt < 1:970334/58‑1 (MQ=39)cacaTCATGATGATTTTCAGCCCGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGtt < 1:3049525/58‑1 (MQ=39)cacaTCATGATGATTTTCAGCCCGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGtt < 1:2469558/58‑1 (MQ=39)cacaTCATGATGATTTTCAGCCCGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGtt < 1:2292960/58‑1 (MQ=39)cacaTCATGATGATTTTCAGCCCGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGtt < 1:1742099/58‑1 (MQ=39)cacaTCATGATGATTTTCAGCCCGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGtt < 1:1366189/58‑1 (MQ=39)cacaTCATGATGATTTTCAGCCCGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGtt < 1:1362386/58‑1 (MQ=39) gatTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTg < 1:1735074/69‑1 (MQ=255) gatTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTg < 1:3089460/69‑1 (MQ=255) gatTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTg < 1:2060894/69‑1 (MQ=255) cccGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACgg < 1:3418793/68‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2916267/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2820836/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2817975/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:3328127/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:1034492/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2737193/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:3108989/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2628138/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:929303/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:1291284/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:2288944/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:1925809/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:544195/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:317995/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:1570227/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:3227292/69‑1 (MQ=255) cccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACCGt < 1:3163005/69‑1 (MQ=255) ccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:188708/68‑1 (MQ=255) ccGTTCTCACCACGTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGt < 1:493064/68‑1 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:3197036/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:3227671/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:2785149/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:2248657/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:731676/1‑68 (MQ=255) ctcGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCa > 1:1453614/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCg > 1:2743188/1‑42 (MQ=37) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:340276/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:2742419/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:340341/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:774937/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:824543/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:3049002/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:3013331/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:2728363/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:272334/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1879779/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1874741/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:179038/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1543061/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1233903/1‑68 (MQ=255) gTTTCAGCCCCTGATGCGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1147270/1‑68 (MQ=255) gTTTCAGCCCCTGATACGCCAGTTCCTCAACCACCGCTTTCGCCTGATCAACGGTACGCACGAACggg > 1:1614958/1‑68 (MQ=255) | CACATCATGATGATTTTCAGCCCGTTCTCGCCACGTTTCAGCCCCTGACGCGCCAGTTCTTCAACCACCGCTTTCGCCTGATCTACGGTACGCACGAACGGG > minE/1112768‑1112869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |